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Article Open Access

4-HPR impairs bladder cancer cell migration and invasion by interfering with the Wnt5a/JNK and Wnt5a/MMP-2 signaling pathways

  • Authors:
    • Yuanfei Cao
    • Xiaolong Wang
    • Chang Xu
    • Zhengyan Gao
    • Haihong Zhou
    • Yongzhi Wang
    • Rui Cao
    • Tao Liu
    • Tongzu Liu
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Taizhou People's Hospital of Jiangsu Province, Taizhou, Jiangsu 225300, P.R. China, Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China
    Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1833-1839
    |
    Published online on: July 22, 2016
       https://doi.org/10.3892/ol.2016.4908
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Abstract

In order to identify the anti-invasive and anti-metastatic effect of the synthetic retinoid N-(4-hydroxyphenyl) retinamide (4-HPR) on the human bladder cancer EJ cell line, and to study its impact on the expression of wingless-type mouse mammary tumor virus integration site family, member 5a (Wnt5a), the phosphorylation of c‑Jun N‑terminal kinase (JNK), the expression levels of matrix metalloproteinase-2 (MMP-2), and the migration and invasion of EJ cells, migration and Matrigel invasion assays, as well as western blot analyses, were used in the present study. The results of the migration and Matrigel invasion assays indicated that the inhibitor of JNK SP600125 could inhibit the effect of 4‑HPR on EJ cells. The expression of Wnt5a and MMP‑2, and the phosphorylation of JNK, were analyzed by western blotting. The data revealed that 4-HPR inhibited the migration and invasion of bladder cancer cells through stimulating Wnt5a activation, causing the downregulation of MMP‑2 expression and enhancing the phosphorylation of JNK in these cells. However, JNK signaling did not appear to have a direct effect on the expression of MMP‑2. The present study demonstrated that 4‑HPR may be a potent anti‑invasive and anti‑metastatic agent that functions via the Wnt5a/JNK and Wnt5a/MMP-2 signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Cao Y, Wang X, Xu C, Gao Z, Zhou H, Wang Y, Cao R, Liu T and Liu T: 4-HPR impairs bladder cancer cell migration and invasion by interfering with the Wnt5a/JNK and Wnt5a/MMP-2 signaling pathways. Oncol Lett 12: 1833-1839, 2016.
APA
Cao, Y., Wang, X., Xu, C., Gao, Z., Zhou, H., Wang, Y. ... Liu, T. (2016). 4-HPR impairs bladder cancer cell migration and invasion by interfering with the Wnt5a/JNK and Wnt5a/MMP-2 signaling pathways. Oncology Letters, 12, 1833-1839. https://doi.org/10.3892/ol.2016.4908
MLA
Cao, Y., Wang, X., Xu, C., Gao, Z., Zhou, H., Wang, Y., Cao, R., Liu, T., Liu, T."4-HPR impairs bladder cancer cell migration and invasion by interfering with the Wnt5a/JNK and Wnt5a/MMP-2 signaling pathways". Oncology Letters 12.3 (2016): 1833-1839.
Chicago
Cao, Y., Wang, X., Xu, C., Gao, Z., Zhou, H., Wang, Y., Cao, R., Liu, T., Liu, T."4-HPR impairs bladder cancer cell migration and invasion by interfering with the Wnt5a/JNK and Wnt5a/MMP-2 signaling pathways". Oncology Letters 12, no. 3 (2016): 1833-1839. https://doi.org/10.3892/ol.2016.4908
Copy and paste a formatted citation
x
Spandidos Publications style
Cao Y, Wang X, Xu C, Gao Z, Zhou H, Wang Y, Cao R, Liu T and Liu T: 4-HPR impairs bladder cancer cell migration and invasion by interfering with the Wnt5a/JNK and Wnt5a/MMP-2 signaling pathways. Oncol Lett 12: 1833-1839, 2016.
APA
Cao, Y., Wang, X., Xu, C., Gao, Z., Zhou, H., Wang, Y. ... Liu, T. (2016). 4-HPR impairs bladder cancer cell migration and invasion by interfering with the Wnt5a/JNK and Wnt5a/MMP-2 signaling pathways. Oncology Letters, 12, 1833-1839. https://doi.org/10.3892/ol.2016.4908
MLA
Cao, Y., Wang, X., Xu, C., Gao, Z., Zhou, H., Wang, Y., Cao, R., Liu, T., Liu, T."4-HPR impairs bladder cancer cell migration and invasion by interfering with the Wnt5a/JNK and Wnt5a/MMP-2 signaling pathways". Oncology Letters 12.3 (2016): 1833-1839.
Chicago
Cao, Y., Wang, X., Xu, C., Gao, Z., Zhou, H., Wang, Y., Cao, R., Liu, T., Liu, T."4-HPR impairs bladder cancer cell migration and invasion by interfering with the Wnt5a/JNK and Wnt5a/MMP-2 signaling pathways". Oncology Letters 12, no. 3 (2016): 1833-1839. https://doi.org/10.3892/ol.2016.4908
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