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Article

MicroRNA-486-5p enhances hepatocellular carcinoma tumor suppression through repression of IGF-1R and its downstream mTOR, STAT3 and c-Myc

  • Authors:
    • Rana Ahmed Youness
    • Hend Mohamed El-Tayebi
    • Reem Amr Assal
    • Karim Hosny
    • Gamal Esmat
    • Ahmed Ihab Abdelaziz
  • View Affiliations / Copyright

    Affiliations: Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo, New Cairo City, Cairo 11835, Egypt, Department of Pharmacology and Toxicology, Faculty of Pharmacy and Biotechnology, German University in Cairo, New Cairo City, Cairo 11835, Egypt, Department of General Surgery, Faculty of Medicine, Cairo University, Giza 12613, Egypt, Department of Endemic Medicine and Hepatology, Cairo University, Giza 12613, Egypt
  • Pages: 2567-2573
    |
    Published online on: July 27, 2016
       https://doi.org/10.3892/ol.2016.4914
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Abstract

The insulin-like growth factor (IGF)-axis has been paradigmatically involved in hepatocellular carcinoma (HCC) tumor initiation, progression and drug resistance. Consequently, members of the IGF‑axis and most importantly, IGF-1 receptor (IGF-1R) have been considered as intriguing targets for HCC therapy. Few miRNAs have been recently reported to be associated with IGF‑1R regulation. The present study aimed to investigate the role of microRNA (miRNA/miR)‑486‑5p in the regulation of IGF‑1R and its downstream signaling cascades. miR‑486‑5p was markedly downregulated in hepatitis C virus‑induced HCC tissues and Huh‑7 cells. Forcing the expression of miR‑486‑5p in Huh‑7 cells resulted in the repression of IGF‑1R, mammalian target of rapamycin (mTOR), signal transducer and activator of transcription 3 (STAT3) and c‑Myc mRNA levels. Ectopic expression of miR‑486‑5p in Huh‑7 cells markedly repressed cellular viability, proliferation, migration and clonogenicity in a similar pattern to IGF‑1R small interfering RNAs, and were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, BrdU incorporation, wound healing and colony forming assays, respectively. Overall, the study findings demonstrated that miR‑486‑5p acts as a tumor suppressor in HCC through the repression of essential members of the IGF‑axis, including IGF‑1R and its downstream mediators mTOR, STAT3 and c‑Myc.
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Copy and paste a formatted citation
Spandidos Publications style
Youness RA, El-Tayebi HM, Assal RA, Hosny K, Esmat G and Abdelaziz AI: MicroRNA-486-5p enhances hepatocellular carcinoma tumor suppression through repression of IGF-1R and its downstream mTOR, STAT3 and c-Myc. Oncol Lett 12: 2567-2573, 2016.
APA
Youness, R.A., El-Tayebi, H.M., Assal, R.A., Hosny, K., Esmat, G., & Abdelaziz, A.I. (2016). MicroRNA-486-5p enhances hepatocellular carcinoma tumor suppression through repression of IGF-1R and its downstream mTOR, STAT3 and c-Myc. Oncology Letters, 12, 2567-2573. https://doi.org/10.3892/ol.2016.4914
MLA
Youness, R. A., El-Tayebi, H. M., Assal, R. A., Hosny, K., Esmat, G., Abdelaziz, A. I."MicroRNA-486-5p enhances hepatocellular carcinoma tumor suppression through repression of IGF-1R and its downstream mTOR, STAT3 and c-Myc". Oncology Letters 12.4 (2016): 2567-2573.
Chicago
Youness, R. A., El-Tayebi, H. M., Assal, R. A., Hosny, K., Esmat, G., Abdelaziz, A. I."MicroRNA-486-5p enhances hepatocellular carcinoma tumor suppression through repression of IGF-1R and its downstream mTOR, STAT3 and c-Myc". Oncology Letters 12, no. 4 (2016): 2567-2573. https://doi.org/10.3892/ol.2016.4914
Copy and paste a formatted citation
x
Spandidos Publications style
Youness RA, El-Tayebi HM, Assal RA, Hosny K, Esmat G and Abdelaziz AI: MicroRNA-486-5p enhances hepatocellular carcinoma tumor suppression through repression of IGF-1R and its downstream mTOR, STAT3 and c-Myc. Oncol Lett 12: 2567-2573, 2016.
APA
Youness, R.A., El-Tayebi, H.M., Assal, R.A., Hosny, K., Esmat, G., & Abdelaziz, A.I. (2016). MicroRNA-486-5p enhances hepatocellular carcinoma tumor suppression through repression of IGF-1R and its downstream mTOR, STAT3 and c-Myc. Oncology Letters, 12, 2567-2573. https://doi.org/10.3892/ol.2016.4914
MLA
Youness, R. A., El-Tayebi, H. M., Assal, R. A., Hosny, K., Esmat, G., Abdelaziz, A. I."MicroRNA-486-5p enhances hepatocellular carcinoma tumor suppression through repression of IGF-1R and its downstream mTOR, STAT3 and c-Myc". Oncology Letters 12.4 (2016): 2567-2573.
Chicago
Youness, R. A., El-Tayebi, H. M., Assal, R. A., Hosny, K., Esmat, G., Abdelaziz, A. I."MicroRNA-486-5p enhances hepatocellular carcinoma tumor suppression through repression of IGF-1R and its downstream mTOR, STAT3 and c-Myc". Oncology Letters 12, no. 4 (2016): 2567-2573. https://doi.org/10.3892/ol.2016.4914
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