MMP-14 and TGFβ-1 methylation in pituitary adenomas

  • Authors:
    • Kornelija Ruskyte
    • Rasa Liutkevicienė
    • Alvita Vilkeviciute
    • Paulina Vaitkiene
    • Indre Valiulytė
    • Brigita Glebauskiene
    • Loresa Kriauciuniene
    • Dalia Zaliuniene
  • View Affiliations

  • Published online on: July 29, 2016     https://doi.org/10.3892/ol.2016.4919
  • Pages: 3013-3017
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Abstract

Pituitary adenoma (PA) is one of the most common abnormalities in the sellar region. Despite the fact that PA is a benign monoclonal neoplasm, it can cause serious complications, including ophthalmological, neurological and endocrinological abnormalities. Currently, the causes that increase the progression of tumors are unknown. Epigenetic silencing of the matrix metalloproteinase‑14 (MMP‑14) and transforming growth factor beta‑1 (TGFβ‑1) genes may be associated with the development of PA, since these genes are important in the processes of tumor metastasis and angiogenesis. The purpose of the present study was to determine if the methylation status of the MMP‑14 and TGFβ‑1 promoters is associated with PA development. In the present study, 120 tissue samples of PA were used. The methylation status of the MMP‑14 and TGFβ‑1 promoters was investigated by methylation specific‑polymerase chain reaction. Statistical analysis was conducted to investigate the associations between the methylation status, age and gender of PA patients, PA tumoral activity, recurrence and invasiveness. The MMP‑14 gene was methylated in 30.00% (17/56 functioning and 19/64 non‑functioning) of patients with PA, while the TGFβ‑1 gene was methylated in 13.33% (9/56 functioning and 7/64 non‑functioning) of patients with PA. It was also observed that promoter methylation of MMP‑14 correlated with the male gender (58.8 vs. 35.7%, P=0.022), while unmethylated (non‑silenced) MMP‑14 correlated with the female gender (64.3 vs. 41.7%, P=0.027). Associations between the promoter methylation status of the MMP‑14 and TGFβ‑1 genes and PA functioning or recurrence were not identified. The present study reveals that silencing of the MMP‑14 gene correlates with patients' gender. However, MMP‑14 and TGFβ‑1 promoter methylation cannot be considered as a prognostic marker in PAs.
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October-2016
Volume 12 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ruskyte K, Liutkevicienė R, Vilkeviciute A, Vaitkiene P, Valiulytė I, Glebauskiene B, Kriauciuniene L and Zaliuniene D: MMP-14 and TGFβ-1 methylation in pituitary adenomas. Oncol Lett 12: 3013-3017, 2016
APA
Ruskyte, K., Liutkevicienė, R., Vilkeviciute, A., Vaitkiene, P., Valiulytė, I., Glebauskiene, B. ... Zaliuniene, D. (2016). MMP-14 and TGFβ-1 methylation in pituitary adenomas. Oncology Letters, 12, 3013-3017. https://doi.org/10.3892/ol.2016.4919
MLA
Ruskyte, K., Liutkevicienė, R., Vilkeviciute, A., Vaitkiene, P., Valiulytė, I., Glebauskiene, B., Kriauciuniene, L., Zaliuniene, D."MMP-14 and TGFβ-1 methylation in pituitary adenomas". Oncology Letters 12.4 (2016): 3013-3017.
Chicago
Ruskyte, K., Liutkevicienė, R., Vilkeviciute, A., Vaitkiene, P., Valiulytė, I., Glebauskiene, B., Kriauciuniene, L., Zaliuniene, D."MMP-14 and TGFβ-1 methylation in pituitary adenomas". Oncology Letters 12, no. 4 (2016): 3013-3017. https://doi.org/10.3892/ol.2016.4919