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Article

Quantitative detection of TUSC3 promoter methylation - a potential biomarker for prognosis in lung cancer

  • Authors:
    • Uta Duppel
    • Matthias Woenckhaus
    • Christian Schulz
    • Johannes Merk
    • Wolfgang Dietmaier
  • View Affiliations / Copyright

    Affiliations: Institute of Pathology, University of Regensburg, D‑93053 Regensburg, Bavaria, Germany, Department of Pathology, Caritas‑Hospital, D‑97980 Bad Mergentheim, Germany, Department of Internal Medicine II, University Hospital Regensburg, D‑93053 Regensburg, Bavaria, Germany, Department of Thoracic Surgery, University Hospital Regensburg, D‑93053 Regensburg, Bavaria, Germany
  • Pages: 3004-3012
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    Published online on: August 1, 2016
       https://doi.org/10.3892/ol.2016.4927
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Abstract

Aberrant promoter methylation of tumor relevant genes frequently occurs in early steps of carcinogenesis and during tumor progression. Epigenetic alterations could be used as potential biomarkers for early detection and for prediction of prognosis and therapy response in lung cancer. The present study quantitatively analyzed the methylation status of known and potential gatekeeper and tumor suppressor genes [O-6-methylguanine-DNA methyltransferase (MGMT), Ras association domain family member 1A (RASSF1A), Ras protein activator like 1 (RASAL1), programmed cell death 4 (PDCD4), metastasis suppressor 1 (MTSS1) and tumor suppressor candidate 3 (TUSC3)] in 42 lung cancers and in corresponding non‑malignant bronchus and lung tissue using bisulfite-conversion independent methylation-quantification of endonuclease-resistant DNA (MethyQESD). Methylation status was associated with clinical and pathological parameters. No methylation was found in the promoter regions of PDCD4 and MTSS1 of either compartment. MGMT, RASSF1A and RASAL1 showed sporadic (up to 26.2%) promoter methylation. The promoter of TUSC3, however, was frequently methylated in the tumor (59.5%), benign bronchus (67.9%) and alveolar lung (31.0%) tissues from each tumor patient. The methylation status of TUSC3 was significantly associated with smaller tumor size (P=0.008) and a longer overall survival (P=0.013). Pooled blood DNA of healthy individuals did not show any methylation of either gene. Therefore, methylation of TUSC3 shows prognostic and pathobiological relevance in lung cancer. Furthermore, quantitative detection of TUSC3 promoter methylation appears to be a promising tool for early detection and prediction of prognosis in lung cancer. However, additional studies are required to confirm this finding.
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1 

Adamietz IA and Niederle N: Lung cancer. Onkologe. 16:615–628. 2010.(In German). View Article : Google Scholar

2 

Herman JG and Baylin SB: Gene silencing in cancer in association with promoter hypermethylation. N Engl J Med. 349:2042–2054. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Licchesi JD, Westra WH, Hooker CM and Herman JG: Promoter hypermethylation of hallmark cancer genes in atypical adenomatous hyperplasia of the lung. Clin Cancer Res. 14:2570–2578. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Yanagawa N, Tamura G, Oizumi H, Kanauchi N, Endoh M, Sadahiro M and Motoyama T: Promoter hypermethylation of RASSF1A and RUNX3 genes as an independent prognostic prediction marker in surgically resected non-small cell lung cancers. Lung Cancer. 58:131–138. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Feng Q, Hawes SE, Stern JE, Wiens L, Lu H, Dong ZM, Jordan CD, Kiviat NB and Vesselle H: DNA methylation in tumor and matched normal tissues from non-small cell lung cancer patients. Cancer Epidemiol Biomarkers Prev. 17:645–654. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Ali AH, Kondo K, Namura T, Senba Y, Takizawa H, Nakagawa Y, Toba H, Kenzaki K, Sakiyama S and Tangoku A: Aberrant DNA methylation of some tumor suppressor genes in lung cancers from workers with chromate exposure. Mol Carcinog. 50:89–99. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Kim H, Kwon YM, Kim JS, Lee H, Park JH, Shim YM, Han J, Park J and Kim DH: Tumor-specific methylation in bronchial lavage for the early detection of non-small-cell lung cancer. J Clin Oncol. 22:2363–2370. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Begum S, Brait M, Dasgupta S, Ostrow KL, Zahurak M, Carvalho AL, Califano JA, Goodman SN, Westra WH, Hoque MO and Sidransky D: An epigenetic marker panel for detection of lung cancer using cell-free serum DNA. Clin Cancer Res. 17:4494–4503. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Leng S, Do K, Yingling CM, Picchi MA, Wolf HJ, Kennedy TC, Feser WJ, Baron AE, Franklin WA, Brock MV, et al: Defining a gene promoter methylation signature in sputum for lung cancer risk assessment. Clin Cancer Res. 18:3387–3395. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Yang J, Shen Y, Liu B and Tong Y: Promoter methylation of BRMS1 correlates with smoking history and poor survival in non-small cell lung cancer patients. Lung Cancer. 74:305–309. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Liu Y, Lan Q, Shen M, Jin J, Mumford J, Ren D and Keohavong P: Aberrant gene promoter methylation in sputum from individuals exposed to smoky coal emissions. Anticancer Res. 28:2061–2066. 2008.PubMed/NCBI

12 

Brabender J, Usadel H, Metzger R, Schneider PM, Park J, Salonga D, TsaoWei DD, Groshen S, Lord RV, Takebe N, et al: Quantitative O(6)-methylguanine DNA methyltransferase methylation analysis in curatively resected non-small cell lung cancer: Associations with clinical outcome. Clin Cancer Res. 9:223–227. 2003.PubMed/NCBI

13 

DeJong WK, Verpooten GF, Kramer H, Louwagie J and Groen HJ: Promoter methylation primarily occurs in tumor cells of patients with non-small cell lung cancer. Anticancer Res. 29:363–369. 2009.PubMed/NCBI

14 

Lin Q, Geng J, Ma K, Yu J, Sun J, Shen Z, Bao G, Chen Y, Zhang H, He Y, et al: RASSF1A, APC, ESR1, ABCB1 and HOXC9, but not p16INK4A, DAPK1, PTEN and MT1G genes were frequently methylated in the stage I non-small cell lung cancer in China. J Cancer Res Clin Oncol. 135:1675–1684. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Maruyama R, Sugio K, Yoshino I, Maehara Y and Gazdar AF: Hypermethylation of FHIT as a prognostic marker in nonsmall cell lung carcinoma. Cancer. 100:1472–1477. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Safar AM, Spencer H III, Su X, Coffey M, Cooney CA, Ratnasinghe LD, Hutchins LF and Fan CY: Methylation profiling of archived non-small cell lung cancer: A promising prognostic system. Clin Cancer Res. 11:4400–4405. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Toyooka S, Maruyama R, Toyooka KO, McLerran D, Feng Z, Fukuyama Y, Virmani AK, ZochbauerMuller S, Tsukuda K, Sugio K, et al: Smoke exposure, histologic type and geography-related differences in the methylation profiles of non-small cell lung cancer. Int J Cancer. 103:153–160. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Jin H, Wang X, Ying J, Wong AH, Cui Y, Srivastava G, Shen ZY, Li EM, Zhang Q, Jin J, et al: Epigenetic silencing of a Ca(2+)-regulated Ras GTPase-activating protein RASAL defines a new mechanism of Ras activation in human cancers. Proc Natl Acad Sci USA. 104:12353–12358. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Ohta M, Seto M, Ijichi H, Miyabayashi K, Kudo Y, Mohri D, Asaoka Y, Tada M, Tanaka Y, Ikenoue T, et al: Decreased expression of the RAS-GTPase activating protein RASAL1 is associated with colorectal tumor progression. Gastroenterology. 136:206–216. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Calvisi DF, Ladu S, Conner EA, Seo D, Hsieh JT, Factor VM and Thorgeirsson SS: Inactivation of Ras GTPase-activating proteins promotes unrestrained activity of wild-type Ras in human liver cancer. J Hepatol. 54:311–319. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Seto M, Ohta M, Ikenoue T, Sugimoto T, Asaoka Y, Tada M, Mohri D, Kudo Y, Ijichi H, Tateishi K, et al: Reduced expression of RAS protein activator like-1 in gastric cancer. Int J Cancer. 128:1293–1302. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Zhang H, Ozaki I, Mizuta T, Hamajima H, Yasutake T, Eguchi Y, Ideguchi H, Yamamoto K and Matsuhashi S: Involvement of programmed cell death 4 in transforming growth factor-betal-induced apoptosis in human hepatocellular carcinoma. Oncogene. 25:6101–6112. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Chen Y, Knösel T, Kristiansen G, Pietas A, Garber ME, Matsuhashi S, Ozaki I and Petersen I: Loss of PDCD4 expression in human lung cancer correlates with tumour progression and prognosis. J Pathol. 200:640–646. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Woenckhaus M, KleinHitpass L, Grepmeier U, Merk J, Pfeifer M, Wild P, Bettstetter M, Wuensch P, Blaszyk H, Hartmann A, et al: Smoking and cancer-related gene expression in bronchial epithelium an non-small-cell lung cancers. J Pathol. 210:192–204. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Dawson JC, Timpson P, Kalna G and Machesky LM: Mtss1 regulates epidermal growth factor signaling in head and neck squamous carcinoma cells. Oncogene. 31:1781–1793. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Liu K, Wang G, Ding H, Chen Y, Yu G and Wang J: Downregulation of metastasis suppressor 1 (MTSS1) is associated with nodal metastasis and poor outcome in Chinese patients with gastric cancer. BMC Cancer. 10:4282010. View Article : Google Scholar : PubMed/NCBI

27 

Xie F, Ye L, Chen J, Wu N, Zhang Z, Yang Y, Zhang L and Jiang WG: The impact of metastasis suppressor-1, MTSS1, on oesophageal squamous cell carcinoma and its clinical significance. J Transl Med. 9:952011. View Article : Google Scholar : PubMed/NCBI

28 

Jahid S, Sun J, Edwards RA, Dizon D, Panarelli NC, Milsom JW, Sikandar SS, Gümüs ZH and Lipkin SM: miR-23a promotes the transition from indolent to invasive colorectal cancer. Cancer Discov. 2:540–553. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Lam DC, Girard L, Suen WS, Chung L, Tin VP, Lam WK, Minna JD and Wong MP: Establishment and expression profiling of new lung cancer cell lines from Chinese smokers and lifetime never-smokers. J Thorac Oncol. 1:932–942. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Bova GS, MacGrogan D, Levy A, Pin SS, Bookstein R and Isaacs WB: Physical mapping of chromosome 8p22 markers and their homozygous deletion in a metastatic prostate cancer. Genomics. 35:46–54. 1996. View Article : Google Scholar : PubMed/NCBI

31 

Pils D, Horak P, Vanhara P, Anees M, Petz M, Alfanz A, Gugerell A, Wittinger M, Gleiss A, Auner V, et al: Methylation status of TUSC3 is a prognostic factor in ovarian cancer. Cancer. 119:946–954. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Ahuja N, Li Q, Mohan AL, Baylin SB and Issa JP: Aging and DNA methylation in colorectal mucosa and cancer. Cancer Res. 58:5489–5494. 1998.PubMed/NCBI

33 

Guervòs MA, Marcos CA, Hermsen M, Nuño AS, Suárez C and Llorente JL: Deletions of N33, STK11 and TP53 are involved in the development of lymph node metastasis in larynx and pharynx carcinomas. Cell Oncol. 29:327–334. 2007.PubMed/NCBI

34 

Scholz C, Nimmrich I, Burger M, Becker E, Dörken B, Ludwig WD and Maier S: Distinction of acute lymphoblastic leukemia from acute myeloid leukemia through microarray-based DNA methylation analysis. Ann Hematol. 84:236–244. 2005. View Article : Google Scholar : PubMed/NCBI

35 

Zemlyakova VV, Zhevlova AI, Zborovskaya IB, Strelnikov VV, Laktionov KK, Zaletaev DV and Nemtsova MV: Methylation profile of several tumor suppressor genes in non-small-cell lung cancer. Mol Biol. 37:836–840. 2003. View Article : Google Scholar

36 

Bettstetter M, Dechant S, Ruemmele P, Vogel C, Kurz K, Morak M, Keller G, HolinskiFeder E, Hofstaedter F and Dietmaier W: MethyQESD, a robust and fast method for quantitative methylation analyses in HNPCC diagnostics using formalin-fixed and paraffin-embedded tissue samples. Lab Invest. 88:1367–1375. 2008. View Article : Google Scholar : PubMed/NCBI

37 

Kubo T, Yamamoto H, Ichimura K, Jida M, Hayashi T, Otani H, Tsukuda K, Sano Y, Kiura K and Toyooka S: DNA methylation in small lung adenocarcinoma with bronchioloalveolar carcinoma components. Lung Cancer. 65:328–332. 2009. View Article : Google Scholar : PubMed/NCBI

38 

Lee SM, Lee WK, Kim DS and Park JY: Quantitative promoter hypermethylation analysis of RASSF1A in lung cancer: Comparison with methylation-specific PCR technique and clinical significance. Mol Med Rep. 5:239–244. 2012.PubMed/NCBI

39 

Kristensen LS, Mikeska T, Krypuy M and Dobrovic A: Sensitive melting analysis after real time-methylation specific PCR (SMART-MSP): High-throughput and probe-free quantitative DNA methylation detection. Nucleic Acids Res. 36:e422008. View Article : Google Scholar : PubMed/NCBI

40 

Grote HJ, Schmiemann V, Kazimirek M and Böcking A: Quantitative methylation-specific PCR for the diagnosis of lung cancer. Pathologe. 28:377–383. 2007.(In German). View Article : Google Scholar : PubMed/NCBI

41 

Cao Z, Yoon JH, Nam SW, Lee JY and Park WS: PDCD4 expression inversely correlated with miR-21 levels in gastric cancers. J Cancer Res Clin Oncol. 138:611–619. 2012. View Article : Google Scholar : PubMed/NCBI

42 

Utikal J, Gratchev A, MullerMolinet I, Oerther S, Kzhyshkowska J, Arens N, Grobholz R, Kannookadan S and Goerdt S: The expression of metastasis suppressor MIM/MTSS1 is regulated by DNA methylation. Int J Cancer. 119:2287–2293. 2006. View Article : Google Scholar : PubMed/NCBI

43 

Fan H, Chen L, Zhang F, Quan Y, Su X, Qiu X, Zhao Z, Kong KL, Dong S, Song Y, et al: MTSS1, a novel target of DNA methyltransferase 3B, functions as a tumor suppressor in hepatocellular carcinoma. Oncogene. 31:2298–2308. 2012. View Article : Google Scholar : PubMed/NCBI

44 

Leupold JH, Asangani IA, Mudduluru G and Allgayer H: Promoter cloning and characterization of the human programmed cell death protein 4 (pdcd4) gene: Evidence for ZBP-89 and Sp-binding motifs as essential Pdcd4 regulators. Biosci Rep. 32:281–297. 2012. View Article : Google Scholar : PubMed/NCBI

45 

Garshasbi M, Hadavi V, Habibi H, Kahrizi K, Kariminejad R, Behjati F, Tzschach A, Najmabadi H, Ropers HH and Kuss AW: A defekt in the TUSC3 gene is associated with autosomal recessive mental retardation. Am J Hum Genet. 82:1158–1164. 2008. View Article : Google Scholar : PubMed/NCBI

46 

Levy A, Dang UC and Bookstein R: High-density screen of human tumor cell lines for homozygous deletions of loci on chromosome arm 8p. Genes Chromosomes Cancer. 24:42–47. 1999. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Duppel U, Woenckhaus M, Schulz C, Merk J and Dietmaier W: Quantitative detection of TUSC3 promoter methylation - a potential biomarker for prognosis in lung cancer. Oncol Lett 12: 3004-3012, 2016.
APA
Duppel, U., Woenckhaus, M., Schulz, C., Merk, J., & Dietmaier, W. (2016). Quantitative detection of TUSC3 promoter methylation - a potential biomarker for prognosis in lung cancer. Oncology Letters, 12, 3004-3012. https://doi.org/10.3892/ol.2016.4927
MLA
Duppel, U., Woenckhaus, M., Schulz, C., Merk, J., Dietmaier, W."Quantitative detection of TUSC3 promoter methylation - a potential biomarker for prognosis in lung cancer". Oncology Letters 12.4 (2016): 3004-3012.
Chicago
Duppel, U., Woenckhaus, M., Schulz, C., Merk, J., Dietmaier, W."Quantitative detection of TUSC3 promoter methylation - a potential biomarker for prognosis in lung cancer". Oncology Letters 12, no. 4 (2016): 3004-3012. https://doi.org/10.3892/ol.2016.4927
Copy and paste a formatted citation
x
Spandidos Publications style
Duppel U, Woenckhaus M, Schulz C, Merk J and Dietmaier W: Quantitative detection of TUSC3 promoter methylation - a potential biomarker for prognosis in lung cancer. Oncol Lett 12: 3004-3012, 2016.
APA
Duppel, U., Woenckhaus, M., Schulz, C., Merk, J., & Dietmaier, W. (2016). Quantitative detection of TUSC3 promoter methylation - a potential biomarker for prognosis in lung cancer. Oncology Letters, 12, 3004-3012. https://doi.org/10.3892/ol.2016.4927
MLA
Duppel, U., Woenckhaus, M., Schulz, C., Merk, J., Dietmaier, W."Quantitative detection of TUSC3 promoter methylation - a potential biomarker for prognosis in lung cancer". Oncology Letters 12.4 (2016): 3004-3012.
Chicago
Duppel, U., Woenckhaus, M., Schulz, C., Merk, J., Dietmaier, W."Quantitative detection of TUSC3 promoter methylation - a potential biomarker for prognosis in lung cancer". Oncology Letters 12, no. 4 (2016): 3004-3012. https://doi.org/10.3892/ol.2016.4927
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