Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring

  • Authors:
    • Jie Zhang
    • Cai Yue Liu
    • Yun Wan
    • Li Peng
    • Wen Fang Li
    • Jia Xuan Qiu
  • View Affiliations

  • Published online on: August 2, 2016     https://doi.org/10.3892/ol.2016.4931
  • Pages: 2835-2839
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The expression of the long non-coding RNA (lncRNA) H19 is associated with proliferation in tumors. In order to investigate whether H19 may additionally mediate the proliferation of fibroblasts in human keloid disease, the present study collected samples from 24 subjects, including 8 with keloids, 8 with normal scars and 8 normal skin controls. Reverse transcription‑polymerase chain reaction revealed that H19 levels were markedly increased in human keloids compared with normal scars and normal skin controls (P=0.017). In order to identify a potential role for H19 in the proliferative activity of human keloid fibroblasts, small interfering (si)RNA‑mediated silencing experiments were performed. H19 siRNA treatment markedly inhibited the proliferation of keloid fibroblasts, as assessed by 3-(4,5-​dimethylthiazol-2-yl)-​2,5-​diphenyltetra­zolium bromide assay (P=0.008). In order to identify the signaling mediators that are regulated by H19 in keloid fibroblasts, the expression levels of mammalian target of rapamycin (mTOR) and vascular endothelial growth factor (VEGF) were examined using western blotting. The results confirmed that knockdown of H19 inhibited mTOR and VEGF expression. In summary, the results indicate that H19 may be associated with increased proliferative activity of keloid fibroblasts, which may be mediated by mTOR and VEGF.
View Figures
View References

Related Articles

Journal Cover

October-2016
Volume 12 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Liu CY, Wan Y, Peng L, Li WF and Qiu JX: Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring. Oncol Lett 12: 2835-2839, 2016
APA
Zhang, J., Liu, C.Y., Wan, Y., Peng, L., Li, W.F., & Qiu, J.X. (2016). Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring. Oncology Letters, 12, 2835-2839. https://doi.org/10.3892/ol.2016.4931
MLA
Zhang, J., Liu, C. Y., Wan, Y., Peng, L., Li, W. F., Qiu, J. X."Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring". Oncology Letters 12.4 (2016): 2835-2839.
Chicago
Zhang, J., Liu, C. Y., Wan, Y., Peng, L., Li, W. F., Qiu, J. X."Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring". Oncology Letters 12, no. 4 (2016): 2835-2839. https://doi.org/10.3892/ol.2016.4931