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Article

Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring

  • Authors:
    • Jie Zhang
    • Cai Yue Liu
    • Yun Wan
    • Li Peng
    • Wen Fang Li
    • Jia Xuan Qiu
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Plastic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China, Department of Plastic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Plastic Surgery, Wenzhou Heping Plastic Surgery Hospital, Wenzhou, Zhejiang 325000, P.R. China
  • Pages: 2835-2839
    |
    Published online on: August 2, 2016
       https://doi.org/10.3892/ol.2016.4931
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Abstract

The expression of the long non-coding RNA (lncRNA) H19 is associated with proliferation in tumors. In order to investigate whether H19 may additionally mediate the proliferation of fibroblasts in human keloid disease, the present study collected samples from 24 subjects, including 8 with keloids, 8 with normal scars and 8 normal skin controls. Reverse transcription‑polymerase chain reaction revealed that H19 levels were markedly increased in human keloids compared with normal scars and normal skin controls (P=0.017). In order to identify a potential role for H19 in the proliferative activity of human keloid fibroblasts, small interfering (si)RNA‑mediated silencing experiments were performed. H19 siRNA treatment markedly inhibited the proliferation of keloid fibroblasts, as assessed by 3-(4,5-​dimethylthiazol-2-yl)-​2,5-​diphenyltetra­zolium bromide assay (P=0.008). In order to identify the signaling mediators that are regulated by H19 in keloid fibroblasts, the expression levels of mammalian target of rapamycin (mTOR) and vascular endothelial growth factor (VEGF) were examined using western blotting. The results confirmed that knockdown of H19 inhibited mTOR and VEGF expression. In summary, the results indicate that H19 may be associated with increased proliferative activity of keloid fibroblasts, which may be mediated by mTOR and VEGF.
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1 

Alster TS and Tanzi EL: Hypertrophic scars and keloids: Etiology and management. Am J Clin Dermatol. 4:235–243. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Wolfram D, Tzankov A, Pülzl P and Piza-Katzer H: Hypertrophic scars and keloids - a review of their pathophysiology, risk factors, and therapeutic management. Dermatol Surg. 35:171–181. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Mofikoya BO, Adeyemo WL and Abdus-salam AA: Keloid and hypertrophic scars: A review of recent developments in pathogenesis and management. Nig Q J Hosp Med. 17:134–139. 2007.PubMed/NCBI

4 

Gibb EA, Brown CJ and Lam WL: The functional role of long non-coding RNA in human carcinomas. Mol Cancer. 10:382011. View Article : Google Scholar : PubMed/NCBI

5 

Prensner JR and Chinnaiyan AM: The emergence of lncRNAs in cancer biology. Cancer Discov. 1:391–407. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Matouk IJ, DeGroot N, Mezan S, Ayesh S, Abulail R, Hochberg A and Galun E: The H19 non-coding RNA is essential for human tumor growth. PLoS One. 2:e8452007. View Article : Google Scholar : PubMed/NCBI

7 

Matouk I, Raveh E, Ohana P, Lail RA, Gershtain E, Gilon M, De Groot N, Czerniak A and Hochberg A: The increasing complexity of the oncofetal h19 gene locus: Functional dissection and therapeutic intervention. Int J Mol Sci. 14:4298–4316. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Lee JY, Yang CC, Chao SC and Wong TW: Histopathological differential diagnosis of keloid and hypertrophic scar. Am J Dermatopathol. 26:379–384. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Monstrey S, Middelkoop E, Vranckx JJ, Bassetto F, Ziegler UE, Meaume S and Téot L: Updated scar management practical guidelines: Non-invasive and invasive measures. J Plast Reconstr Aesthet Surg. 67:1017–1025. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Yang F, Bi J, Xue X, Zheng L, Zhi K, Hua J and Fang G: Upregulated long non-coding RNA H19 contributes to proliferation of gastric cancer cells. FEBS J. 279:3159–3165. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Kruger NJ: The Bradford method for protein quantitation. Methods Mol Biol. 32:9–15. 1994.PubMed/NCBI

12 

Calderon M, Lawrence WT and Banes AJ: Increased proliferation in keloid fibroblasts wounded in vitro. J Surg Res. 61:343–347. 1996. View Article : Google Scholar : PubMed/NCBI

13 

Ong CT, Khoo YT, Mukhopadhyay A, Do DV, Lim IJ, Aalami O and Phan TT: mTOR as a potential therapeutic target for treatment of keloids and excessive scars. Exp Dermatol. 16:394–404. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Laplante M and Sabatini DM: mTOR signaling in growth control and disease. Cell. 149:274–293. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Clark JA, Leung KS, Cheng JC and Leung PC: The hypertrophic scar and microcirculation properties. Burns. 22:447–450. 1996. View Article : Google Scholar : PubMed/NCBI

16 

Wu WS, Wang FS, Yang KD, Huang CC and Kuo YR: Dexamethasone induction of keloid regression through effective suppression of VEGF expression and keloid fibroblast proliferation. J Invest Dermatol. 126:1264–1271. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Ferrara N, Gerber HP and LeCouter J: The biology of VEGF and its receptors. Nat Med. 9:669–676. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Crafts TD, Jensen AR, BlocherSmith EC and Markel TA: Vascular endothelial growth factor: Therapeutic possibilities and challenges for the treatment of ischemia. Cytokine. 71:385–393. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Otrock ZK, Makarem JA and Shamseddine AI: Vascular endothelial growth factor family of ligands and receptors: Review. Blood Cells Mol Dis. 38:258–268. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Otrock ZK, Mahfouz RA, Makarem JA and Shamseddine AI: Understanding the biology of angiogenesis: Review of the most important molecular mechanisms. Blood Cells Mol Dis. 39:212–220. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Farhat FS, Tfayli A, Fakhruddin N, Mahfouz R, Otrock ZK, Alameddine RS, Awada AH and Shamseddine A: Expression, prognostic and predictive impact of VEGF and bFGF in non-small cell lung cancer. Crit Rev Oncol Hematol. 84:149–160. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Trompezinski S, Denis A, Vinche A, Schmitt D and Viac J: IL-4 and interferon-gamma differentially modulate vascular endothelial growth factor release from normal human keratinocytes and fibroblasts. Exp Dermatol. 11:224–231. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Trompezinski S, BerthierVergnes O, Denis A, Schmitt D and Viac J: Comparative expression of vascular endothelial growth factor family members, VEGF-B, -C and -D, by normal human keratinocytes and fibroblasts. Exp Dermatol. 13:98–105. 2004. View Article : Google Scholar : PubMed/NCBI

24 

DeFelice B, Garbi C, Wilson RR, Santoriello M and Nacca M: Effect of selenocystine on gene expression profiles in human keloid fibroblasts. Genomics. 97:265–276. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Hu Z, Lou L and Luo S: Experimental study of the expression of c-myc, c-fos and proto-oncogenes on hypertrophic and scars. Zhonghua Zheng Xing Wai Ke Za Zhi. 18:165–167. 2002.(In Chinese). PubMed/NCBI

26 

Bretones G, Delgado MD and León J: Myc and cell cycle control. Biochim Biophys Acta. 1849:506–516. 2014. View Article : Google Scholar : PubMed/NCBI

27 

BarsyteLovejoy D, Lau SK, Boutros PC, Khosravi F, Jurisica I, Andrulis IL, Tsao MS and Penn LZ: The c-Myc oncogene directly induces the H19 noncoding RNA by allele-specific binding to potentiate tumorigenesis. Cancer Res. 66:5330–5337. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Luo X, Pan Q, Liu L and Chegini N: Genomic and proteomic profiling II: Comparative assessment of gene expression profiles in leiomyomas, keloids, and surgically-induced scars. Reprod Biol Endocrinol. 5:352007. View Article : Google Scholar : PubMed/NCBI

29 

Berteaux N, Lottin S, Monté D, Pinte S, Quatannens B, Coll J, Hondermarck H, Curgy JJ, Dugimont T and Adriaenssens E: H19 mRNA-like noncoding RNA promotes breast cancer cell proliferation through positive control by E2F1. J Biol Chem. 280:29625–29636. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Liu CY, Wan Y, Peng L, Li WF and Qiu JX: Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring. Oncol Lett 12: 2835-2839, 2016.
APA
Zhang, J., Liu, C.Y., Wan, Y., Peng, L., Li, W.F., & Qiu, J.X. (2016). Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring. Oncology Letters, 12, 2835-2839. https://doi.org/10.3892/ol.2016.4931
MLA
Zhang, J., Liu, C. Y., Wan, Y., Peng, L., Li, W. F., Qiu, J. X."Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring". Oncology Letters 12.4 (2016): 2835-2839.
Chicago
Zhang, J., Liu, C. Y., Wan, Y., Peng, L., Li, W. F., Qiu, J. X."Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring". Oncology Letters 12, no. 4 (2016): 2835-2839. https://doi.org/10.3892/ol.2016.4931
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Liu CY, Wan Y, Peng L, Li WF and Qiu JX: Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring. Oncol Lett 12: 2835-2839, 2016.
APA
Zhang, J., Liu, C.Y., Wan, Y., Peng, L., Li, W.F., & Qiu, J.X. (2016). Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring. Oncology Letters, 12, 2835-2839. https://doi.org/10.3892/ol.2016.4931
MLA
Zhang, J., Liu, C. Y., Wan, Y., Peng, L., Li, W. F., Qiu, J. X."Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring". Oncology Letters 12.4 (2016): 2835-2839.
Chicago
Zhang, J., Liu, C. Y., Wan, Y., Peng, L., Li, W. F., Qiu, J. X."Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring". Oncology Letters 12, no. 4 (2016): 2835-2839. https://doi.org/10.3892/ol.2016.4931
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