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Article Open Access

Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma

  • Authors:
    • Junguo Wang
    • Fang Mei
    • Xia Gao
    • Shoulin Wang
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology‑Head and Neck Surgery, Drum Tower Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China, Department of Ophthalmology, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China, Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2375-2380
    |
    Published online on: August 3, 2016
       https://doi.org/10.3892/ol.2016.4940
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Abstract

Nasopharyngeal carcinoma (NPC) is the most common cancer originating from the nasopharynx, and can be induced by infection with Epstein‑Barr virus (EBV). To study the mechanisms of EBV‑associated NPC, a microarray of the GSE12452 dataset was analyzed. GSE12452 was downloaded from Gene Expression Omnibus and consisted of 31 NPC samples and 10 normal healthy nasopharyngeal tissue samples. The differentially‑expressed genes (DEGs) were screened using the linear models for microarray data package in R. Using Database for Annotation, Visualization and Integrated Discovery software, potential functions of the DEGs were predicted by Gene Ontology and pathway enrichment analyses. With the information from the Search Tool for the Retrieval of Interacting Genes/Proteins database, the protein‑protein interaction (PPI) network was visualized by Cytoscape. Furthermore, modules of the PPI network were searched using ClusterONE in Cytoscape. A total of 951 DEGs were screened in the NPC samples compared with the normal healthy nasopharyngeal tissue samples. Function enrichment indicated that the upregulated genes were associated with the cell cycle, cytoskeleton organization and DNA metabolism. Meanwhile, the downregulated genes were mainly associated with cell differentiation, hormone metabolism, inflammatory response and immune response. PPI networks for the DEGs suggested that upregulated mitotic arrest deficient 2‑like 1 (MAD2L1; degree=133), proliferating cell nuclear antigen (PCNA; degree=125) and cyclin B1 (CCNB1; degree=115), and downregulated member A1 of aldehyde dehydrogenase 1 (ALDH1A1; degree=15) may be of great importance as they exhibited higher degrees on interaction. Mucin 1 (MUC1) was a key node of module 4. Overall, the study indicated that MAD2L1, CCNB1, PCNA, ALDH1A1 and MUC1 may have a correlation with EBV‑associated NPC.
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View References

1 

Xia H, Ng SS, Jiang S, Cheung WK, Sze J, Bian XW, Kung HF and Lin MC: miR-200a-mediated downregulation of ZEB2 and CTNNB1 differentially inhibits nasopharyngeal carcinoma cell growth, migration and invasion. Biochem Biophys Res Commun. 391:535–541. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Zhang F and Zhang J: Clinical hereditary characteristics in nasopharyngeal carcinoma through Ye-Liang's family cluster. Chin Med J (Engl). 112:185–187. 1999.PubMed/NCBI

3 

Lo KW, Chung GT and To KF: Deciphering the molecular genetic basis of NPC through molecular, cytogenetic, and epigenetic approaches. Semin Cancer Biol. 22:79–86. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Yu MC, Ho JH, Lai SH and Henderson BE: Cantonese-style salted fish as a cause of nasopharyngeal carcinoma: Report of a case-control study in Hong Kong. Cancer Res. 46:956–961. 1986.PubMed/NCBI

5 

Lozano R, Naghavi M, Foreman K, Lim S, Shibuya K, Aboyans V, Abraham J, Adair T, Aggarwal R, Ahn SY, et al: Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: A systematic analysis for the global burden of disease study 2010. Lancet. 380:2095–2128. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Wei MX, Moulin JC, Decaussin G, Berger F and Ooka T: Expression and tumorigenicity of the Epstein-Barr virus BARF1 gene in human Louckes B-lymphocyte cell line. Cancer Res. 54:1843–1848. 1994.PubMed/NCBI

7 

Decaussin G, SbihLammali F, de Turenne-Tessier M, Bouguermouh A and Ooka T: Expression of BARF1 gene encoded by Epstein-Barr virus in nasopharyngeal carcinoma biopsies. Cancer Res. 60:5584–5588. 2000.PubMed/NCBI

8 

Seto E, Yang L, Middeldorp J, Sheen TS, Chen JY, Fukayama M, Eizuru Y, Ooka T and Takada K: Epstein-Barr virus (EBV)-encoded BARF1 gene is expressed in nasopharyngeal carcinoma and EBV-associated gastric carcinoma tissues in the absence of lytic gene expression. J Med Virol. 76:82–88. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Zhou L, Jiang W, Ren C, Yin Z, Feng X, Liu W, Tao Q and Yao K: Frequent hypermethylation of RASSF1A and TSLC1, and high viral load of Epstein-Barr Virus DNA in nasopharyngeal carcinoma and matched tumor-adjacent tissues. Neoplasia. 7:809–815. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Lo KW, Kwong J, Hui AB, Chan SY, To KF, Chan AS, Chow LS, Teo PM, Johnson PJ and Huang DP: High frequency of promoter hypermethylation of RASSF1A in nasopharyngeal carcinoma. Cancer Res. 61:3877–3881. 2001.PubMed/NCBI

11 

Feng P, Ren EC, Liu D, Chan SH and Hu H: Expression of Epstein-Barr virus lytic gene BRLF1 in nasopharyngeal carcinoma: Potential use in diagnosis. J Gen Virol. 81:2417–2423. 2000. View Article : Google Scholar : PubMed/NCBI

12 

Horikawa T, Sheen TS, Takeshita H, Sato H, Furukawa M and Yoshizaki T: Induction of c-Met proto-oncogene by Epstein-Barr virus latent membrane protein-1 and the correlation with cervical lymph node metastasis of nasopharyngeal carcinoma. Am J Pathol. 159:27–33. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Heussinger N, Büttner M, Ott G, Brachtel E, Pilch BZ, Kremmer E and Niedobitek G: Expression of the Epstein-Barr virus (EBV)-encoded latent membrane protein 2A (LMP2A) in EBV-associated nasopharyngeal carcinoma. J Pathol. 203:696–699. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Sengupta S, den Boon JA, Chen IH, Newton MA, Dahl DB, Chen M, Cheng YJ, Westra WH, Chen CJ, Hildesheim A, et al: Genome-wide expression profiling reveals EBV-associated inhibition of MHC class I expression in nasopharyngeal carcinoma. Cancer Res. 66:7999–8006. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Gautier L, Cope L, Bolstad BM and Irizarry RA: Affy-analysis of Affymetrix GeneChip data at the probe level. Bioinformatics. 20:307–315. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Bolstad BM, Irizarry RA, Åstrand M and Speed TP: A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics. 19:185–193. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Smyth GK: Limma: Linear models for microarray dataBioinformatics and Computational Biology Solutions using R and Bioconductor. Gentleman R, Carey V, Dudoit S, Irizarry R and Huber W: Springer; New York: pp. 397–420. 2005, View Article : Google Scholar

18 

Tweedie S, Ashburner M, Falls K, Leyland P, McQuilton P, Marygold S, Millburn G, OsumiSutherland D, Schroeder A, Seal R, et al: FlyBase: Enhancing Drosophila gene ontology annotations. Nucleic Acids Res. 37:D555–D559. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Kanehisa M and Goto S: KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 28:27–30. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Dennis G Jr, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC and Lempicki RA: DAVID: Database for annotation, visualization, and integrated discovery. Genome Biol. 4:P32003. View Article : Google Scholar : PubMed/NCBI

21 

Franceschini A, Szklarczyk D, Frankild S, Kuhn M, Simonovic M, Roth A, Lin J, Minguez P, Bork P, von Mering C and Jensen LJ: STRING v9. 1: Protein-protein interaction networks, with increased coverage and integration. Nucleic Acids Res. 41:D808–D815. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Smoot ME, Ono K, Ruscheinski J, Wang PL and Ideker T: Cytoscape 2.8: New features for data integration and network visualization. Bioinformatics. 27:431–432. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Nepusz T, Yu H and Paccanaro A: Detecting overlapping protein complexes in protein-protein interaction networks. Nat Methods. 9:471–472. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Cheung HW, Jin DY, Ling MT, Wong YC, Wang Q, Tsao SW and Wang X: Mitotic arrest deficient 2 expression induces chemosensitization to a DNA-damaging agent, cisplatin, in nasopharyngeal carcinoma cells. Cancer Res. 65:1450–1458. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Wang X, Jin DY, Wong YC, Cheung AL, Chun AC, Lo AK, Liu Y and Tsao SW: Correlation of defective mitotic checkpoint with aberrantly reduced expression of MAD2 protein in nasopharyngeal carcinoma cells. Carcinogenesis. 21:2293–2297. 2000. View Article : Google Scholar : PubMed/NCBI

26 

Wang X, Jin DY, Wong HL, Feng H, Wong YC and Tsao SW: MAD2-induced sensitization to vincristine is associated with mitotic arrest and Raf/Bcl-2 phosphorylation in nasopharyngeal carcinoma cells. Oncogene. 22:109–116. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Huang TS, Shu CH, Chao Y, Chen SN and Chen LL: Activation of MAD 2 checkprotein and persistence of cyclin B1/CDC 2 activity associate with paclitaxel-induced apoptosis in human nasopharyngeal carcinoma cells. Apoptosis. 5:235–241. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Tsai ST, Jin YT, Leung HW, Wang ST, Tsao CJ and Su IJ: Bcl-2 and proliferating cell nuclear antigen (PCNA) expression correlates with subsequent local recurrence in nasopharyngeal carcinomas. Anticancer Res. 18:2849–2854. 1998.PubMed/NCBI

29 

Lian B, Wang JQ and Jin L: Effects of siRNA targeting PCNA gene on nasopharyngeal carcinoma CNE2 cell cycle. Chin J Pathophysiol. 25:1533–1537. 2009.(In Chinese).

30 

Luo WR and Yao KT: Cancer stem cell characteristics, ALDH1 expression in the invasive front of nasopharyngeal carcinoma. Virchows Arch. 464:35–43. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Wu A, Luo W, Zhang Q, Yang Z, Zhang G, Li S and Yao K: Aldehyde dehydrogenase 1, a functional marker for identifying cancer stem cells in human nasopharyngeal carcinoma. Cancer Lett. 330:181–189. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Luo WR, Gao F, Li SY and Yao KT: Tumour budding and the expression of cancer stem cell marker aldehyde dehydrogenase 1 in nasopharyngeal carcinoma. Histopathology. 61:1072–1081. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Hou W, He W, Li Y, Ma R, Wang Z, Zhu X, Fu Q, Wen Y, Li H and Wen W: Increased expression of aldehyde dehydrogenase 1 A1 in nasopharyngeal carcinoma is associated with enhanced invasiveness. Eur Arch Otorhinolaryngol. 271:171–179. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Kondo S, Yoshizaki T, Wakisaka N, Horikawa T, Murono S, Jang KL, Joab I, Furukawa M and Pagano JS: MUC1 induced by Epstein-Barr virus latent membrane protein 1 causes dissociation of the cell-matrix interaction and cellular invasiveness via STAT signaling. J Virol. 81:1554–1562. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Mei F, Gao X and Wang S: Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma. Oncol Lett 12: 2375-2380, 2016.
APA
Wang, J., Mei, F., Gao, X., & Wang, S. (2016). Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma. Oncology Letters, 12, 2375-2380. https://doi.org/10.3892/ol.2016.4940
MLA
Wang, J., Mei, F., Gao, X., Wang, S."Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma". Oncology Letters 12.4 (2016): 2375-2380.
Chicago
Wang, J., Mei, F., Gao, X., Wang, S."Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma". Oncology Letters 12, no. 4 (2016): 2375-2380. https://doi.org/10.3892/ol.2016.4940
Copy and paste a formatted citation
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Spandidos Publications style
Wang J, Mei F, Gao X and Wang S: Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma. Oncol Lett 12: 2375-2380, 2016.
APA
Wang, J., Mei, F., Gao, X., & Wang, S. (2016). Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma. Oncology Letters, 12, 2375-2380. https://doi.org/10.3892/ol.2016.4940
MLA
Wang, J., Mei, F., Gao, X., Wang, S."Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma". Oncology Letters 12.4 (2016): 2375-2380.
Chicago
Wang, J., Mei, F., Gao, X., Wang, S."Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma". Oncology Letters 12, no. 4 (2016): 2375-2380. https://doi.org/10.3892/ol.2016.4940
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