Open Access

Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma

  • Authors:
    • Junguo Wang
    • Fang Mei
    • Xia Gao
    • Shoulin Wang
  • View Affiliations

  • Published online on: August 3, 2016     https://doi.org/10.3892/ol.2016.4940
  • Pages: 2375-2380
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Nasopharyngeal carcinoma (NPC) is the most common cancer originating from the nasopharynx, and can be induced by infection with Epstein‑Barr virus (EBV). To study the mechanisms of EBV‑associated NPC, a microarray of the GSE12452 dataset was analyzed. GSE12452 was downloaded from Gene Expression Omnibus and consisted of 31 NPC samples and 10 normal healthy nasopharyngeal tissue samples. The differentially‑expressed genes (DEGs) were screened using the linear models for microarray data package in R. Using Database for Annotation, Visualization and Integrated Discovery software, potential functions of the DEGs were predicted by Gene Ontology and pathway enrichment analyses. With the information from the Search Tool for the Retrieval of Interacting Genes/Proteins database, the protein‑protein interaction (PPI) network was visualized by Cytoscape. Furthermore, modules of the PPI network were searched using ClusterONE in Cytoscape. A total of 951 DEGs were screened in the NPC samples compared with the normal healthy nasopharyngeal tissue samples. Function enrichment indicated that the upregulated genes were associated with the cell cycle, cytoskeleton organization and DNA metabolism. Meanwhile, the downregulated genes were mainly associated with cell differentiation, hormone metabolism, inflammatory response and immune response. PPI networks for the DEGs suggested that upregulated mitotic arrest deficient 2‑like 1 (MAD2L1; degree=133), proliferating cell nuclear antigen (PCNA; degree=125) and cyclin B1 (CCNB1; degree=115), and downregulated member A1 of aldehyde dehydrogenase 1 (ALDH1A1; degree=15) may be of great importance as they exhibited higher degrees on interaction. Mucin 1 (MUC1) was a key node of module 4. Overall, the study indicated that MAD2L1, CCNB1, PCNA, ALDH1A1 and MUC1 may have a correlation with EBV‑associated NPC.
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October-2016
Volume 12 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wang J, Mei F, Gao X and Wang S: Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma. Oncol Lett 12: 2375-2380, 2016
APA
Wang, J., Mei, F., Gao, X., & Wang, S. (2016). Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma. Oncology Letters, 12, 2375-2380. https://doi.org/10.3892/ol.2016.4940
MLA
Wang, J., Mei, F., Gao, X., Wang, S."Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma". Oncology Letters 12.4 (2016): 2375-2380.
Chicago
Wang, J., Mei, F., Gao, X., Wang, S."Identification of genes involved in Epstein‑Barr virus‑associated nasopharyngeal carcinoma". Oncology Letters 12, no. 4 (2016): 2375-2380. https://doi.org/10.3892/ol.2016.4940