Tumor‑suppressing effects of microRNA‑429 in human renal cell carcinoma via the downregulation of Sp1

  • Authors:
    • Deyao Wu
    • Xiaobing Niu
    • Huixing Pan
    • Yunfeng Zhou
    • Zichun Zhang
    • Ping Qu
    • Jian Zhou
  • View Affiliations

  • Published online on: August 5, 2016     https://doi.org/10.3892/ol.2016.4953
  • Pages: 2906-2911
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Abstract

MicroRNA (miR)-429 has been frequently reported to be downregulated in various tumors, including renal cell carcinoma (RCC), nasopharyngeal carcinoma, Ehrlich ascites tumor cells, gastric cancer, non‑small cell lung cancer and endometrial endometrioid carcinoma. The present study investigated the effects of miR‑429 on human RCC A498 and 786‑O cells. Following transfection of cells with miR‑429 mimics and scrambled control, MTT, cell migration, cell invasion and luciferase assays were performed. In addition, western blotting was performed in order to assess the expression of specificity protein 1 (Sp1), which was predicted to be a target of miR‑429 by TargetScan. The present results revealed that miR‑429 inhibited cell proliferation, migration and invasion of 786‑O and A498 cells. In addition, the present results demonstrated that miR‑429 overexpression downregulated Sp1 protein expression, which provides evidence that miR‑429 may directly target Sp1 in RCC. These results suggest that miR‑429 may be investigated for use as a predictive marker for early detection of tumor metastasis and blocking RCC cells from becoming invasive.
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October-2016
Volume 12 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wu D, Niu X, Pan H, Zhou Y, Zhang Z, Qu P and Zhou J: Tumor‑suppressing effects of microRNA‑429 in human renal cell carcinoma via the downregulation of Sp1. Oncol Lett 12: 2906-2911, 2016
APA
Wu, D., Niu, X., Pan, H., Zhou, Y., Zhang, Z., Qu, P., & Zhou, J. (2016). Tumor‑suppressing effects of microRNA‑429 in human renal cell carcinoma via the downregulation of Sp1. Oncology Letters, 12, 2906-2911. https://doi.org/10.3892/ol.2016.4953
MLA
Wu, D., Niu, X., Pan, H., Zhou, Y., Zhang, Z., Qu, P., Zhou, J."Tumor‑suppressing effects of microRNA‑429 in human renal cell carcinoma via the downregulation of Sp1". Oncology Letters 12.4 (2016): 2906-2911.
Chicago
Wu, D., Niu, X., Pan, H., Zhou, Y., Zhang, Z., Qu, P., Zhou, J."Tumor‑suppressing effects of microRNA‑429 in human renal cell carcinoma via the downregulation of Sp1". Oncology Letters 12, no. 4 (2016): 2906-2911. https://doi.org/10.3892/ol.2016.4953