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BCAT1 promotes tumor cell migration and invasion in hepatocellular carcinoma

  • Authors:
    • Meng Xu
    • Qingquan Liu
    • Yuli Jia
    • Kangsheng Tu
    • Yingmin Yao
    • Qingguang Liu
    • Cheng Guo
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2648-2656
    |
    Published online on: August 8, 2016
       https://doi.org/10.3892/ol.2016.4969
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Abstract

Branched‑chain amino acid transaminase 1 (BCAT1) has been associated with numerous types of tumors; however, few previous studies have evaluated the expression and role of BCAT1 in hepatocellular carcinoma (HCC). In the present study, the expression of BCAT1 was detected by reverse transcription‑quantitative polymerase chain reaction and immunoblotting in six HCC cell lines and 74 pairs of HCC and adjacent non‑cancerous liver tissues. In addition, the correlation between the expression levels of c‑Myc and BCAT1 was analyzed using immunohistochemistry. Furthermore, RNA silencing was performed using c‑Myc‑specific or BCAT1‑specific small interfering RNA, after which wound healing and Transwell cell invasion assays were performed. Finally, the clinicopathological characteristics of BCAT1 in patients with HCC were analyzed. It was shown that the expression of BCAT1 was significantly higher in HCC tissues compared with adjacent non‑tumor tissues (P<0.001), and in HCC cell lines compared within the L‑02 hepatic cell line (P<0.001). In addition, immunohistochemical analyses indicated that the expression of BCAT1 was positively correlated with c‑Myc (r=0.706, P<0.001). BCAT1 expression was shown to be downregulated in c‑Myc‑knockdown cells, and silencing of BCAT1 expression reduced the invasion and migration of HCC cells. Furthermore, a clinical analysis indicated that BCAT1 expression in HCC tissues was significantly associated with the tumor‑node‑metastasis stage, tumor number and tumor differentiation (all P<0.05), and that BCAT1 was able to predict the 5‑year survival and disease‑free survival rates of patients with HCC (both P<0.001). The results of the present study suggested that BCAT1 expression is upregulated in patients with HCC, and that BCAT1 may serve as a potential molecular target for the diagnosis and treatment of HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Xu M, Liu Q, Jia Y, Tu K, Yao Y, Liu Q and Guo C: BCAT1 promotes tumor cell migration and invasion in hepatocellular carcinoma. Oncol Lett 12: 2648-2656, 2016.
APA
Xu, M., Liu, Q., Jia, Y., Tu, K., Yao, Y., Liu, Q., & Guo, C. (2016). BCAT1 promotes tumor cell migration and invasion in hepatocellular carcinoma. Oncology Letters, 12, 2648-2656. https://doi.org/10.3892/ol.2016.4969
MLA
Xu, M., Liu, Q., Jia, Y., Tu, K., Yao, Y., Liu, Q., Guo, C."BCAT1 promotes tumor cell migration and invasion in hepatocellular carcinoma". Oncology Letters 12.4 (2016): 2648-2656.
Chicago
Xu, M., Liu, Q., Jia, Y., Tu, K., Yao, Y., Liu, Q., Guo, C."BCAT1 promotes tumor cell migration and invasion in hepatocellular carcinoma". Oncology Letters 12, no. 4 (2016): 2648-2656. https://doi.org/10.3892/ol.2016.4969
Copy and paste a formatted citation
x
Spandidos Publications style
Xu M, Liu Q, Jia Y, Tu K, Yao Y, Liu Q and Guo C: BCAT1 promotes tumor cell migration and invasion in hepatocellular carcinoma. Oncol Lett 12: 2648-2656, 2016.
APA
Xu, M., Liu, Q., Jia, Y., Tu, K., Yao, Y., Liu, Q., & Guo, C. (2016). BCAT1 promotes tumor cell migration and invasion in hepatocellular carcinoma. Oncology Letters, 12, 2648-2656. https://doi.org/10.3892/ol.2016.4969
MLA
Xu, M., Liu, Q., Jia, Y., Tu, K., Yao, Y., Liu, Q., Guo, C."BCAT1 promotes tumor cell migration and invasion in hepatocellular carcinoma". Oncology Letters 12.4 (2016): 2648-2656.
Chicago
Xu, M., Liu, Q., Jia, Y., Tu, K., Yao, Y., Liu, Q., Guo, C."BCAT1 promotes tumor cell migration and invasion in hepatocellular carcinoma". Oncology Letters 12, no. 4 (2016): 2648-2656. https://doi.org/10.3892/ol.2016.4969
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