Effects of ZNF139 on gastric cancer cells and mice with gastric tumors

Nie,Hong‑Feng; Li,Yong; Li,Zhen‑Xing; Mu,Ji‑Xing; Wang,Jin‑Sheng

doi:10.3892/ol.2016.4982

October-2016 Volume 12 Issue 4

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

October-2016 Volume 12 Issue 4

Full Size Image

Article Open Access

Effects of ZNF139 on gastric cancer cells and mice with gastric tumors

Authors:
- Hong‑Feng Nie
- Yong Li
- Zhen‑Xing Li
- Ji‑Xing Mu
- Jin‑Sheng Wang
View Affiliations / Copyright

Affiliations: Department of General Surgery, The First Affiliated Hospital, Xingtai Medical College, Xingtai, Hebei 054001, P.R. China, Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of General Surgery, Hebei United University Affiliated Hospital, Tangshan, Hebei 063000, P.R. China

Copyright: © Nie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 2550-2554
|
Published online on: August 10, 2016

https://doi.org/10.3892/ol.2016.4982
Expand metrics +

Abstract

Gastric cancer (GC) is the fourth most common type of cancer, worldwide. The major molecular factors associated with the pathogenesis of GC remain unclear. Previous studies found that zinc finger proteins are highly abundant in human eukaryotes and tissues, and play an important role in maintaining normal cellular functions and have an association with tumor initiation. In the current study, interference technology was used to silence the ZNF139 protein, a zinc finger protein that was previously found to be closely associated with GC. The results showed that cell viability and proliferation were inhibited in the Znf139‑knockdown of GC cells. Additional study found that the expression levels of B cell lymphoma‑2 (Bcl‑2) and survivin messenger RNAs and proteins were downregulated in Znf139‑silenced cells, indicating that cells expression Znf139 are able to induce the growth of tumor cells by mediating the apoptosis pathway. Further in vivo experiments showed that Znf139 knockdown downregulated the expression levels of Bcl‑2 and survivin in mice. Overall, the in vitro and in vivo findings of the present study indicate that ZNF139 may be actively involved in the progression of GC.

View Figures

View References

1	Ma Z, Wang W, Jin G, Chu P and Li H: Effect of statins on gastric cancer incidence: A meta-Analysis of case control studies. J Cancer Res Ther. 10:859–865. 2014. View Article : Google Scholar : PubMed/NCBI
2	Imamura T, Komatsu S, Ichikawa D, Kubota T, Okamoto K, Konishi H, Shiozaki A, Fujiwara H, Morimura R, Murayama Y, et al: Poor prognostic subgroup in T3N0 stage IIA gastric cancer, suggesting an indication for adjuvant chemotherapy. J Surg Oncol. 111:221–225. 2015. View Article : Google Scholar : PubMed/NCBI
3	Wei S, Zhang L, Zhou X, Du M, Jiang Z, Hausman GJ, Bergen WG, Zan L and Dodson MV: Emerging roles of zinc finger proteins in regulating adipogenesis. Cell Mol Life Sci. 70:4569–4584. 2013. View Article : Google Scholar : PubMed/NCBI
4	Singh PK, Srivastava AK, Dalela D, Rath SK, Goel MM and Bhatt ML: Frequent expression of zinc-finger protein ZNF165 in human urinary bladder transitional cell carcinoma. Immunobiology. 220:68–73. 2015. View Article : Google Scholar : PubMed/NCBI
5	Li P, Maines-Bandiera S, Kuo WL, Guan Y, Sun Y, Hills M, Huang G, Collins CC, Leung PC, Gray JW and Auersperg N: Multiple roles of the candidate oncogene ZNF217 in ovarian epithelial neoplastic progression. Int J Cancer. 120:1863–1873. 2007. View Article : Google Scholar : PubMed/NCBI
6	Li Y, Tan BB, Zhao Q, Fan LQ, Wang D and Liu Y: ZNF139 promotes tumor metastasis by increasing migration and invasion in human gastric cancer cells. Neoplasma. 61:291–298. 2014. View Article : Google Scholar : PubMed/NCBI
7	Zhang S, Yang JH, Guo CK and Cai PC: Gene silencing of TKTL1 by RNAi inhibits cell proliferation in human hepatoma cells. Cancer Lett. 253:108–114. 2007. View Article : Google Scholar : PubMed/NCBI
8	Livak and Schmittgen, . Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
9	Fock KM: Review article: The epidemiology and prevention of gastric cancer. Aliment Pharmacol Ther. 40:250–260. 2014. View Article : Google Scholar : PubMed/NCBI
10	Zhang S, Zuo L, Gui S, Zhou Q, Wei W and Wang Y: Induction of cell differentiation and promotion of endocan gene expression in stomach cancer by melatonin. Mol Biol Rep. 39:2843–2892. 2012. View Article : Google Scholar : PubMed/NCBI
11	vanDekken H, Tilanus HW, Hop WC, Dinjens WN, Wink JC, Vissers KJ and van Marion R: Array comparative genomic hybridization, expression array, and protein analysis of critical regions on chromosome arms 1q, 7q, and 8p in adenocarcinomas of the gastroesophageal junction. Cancer Genet Cytogenet. 189:37–42. 2009. View Article : Google Scholar : PubMed/NCBI
12	Li Y, Tan BB, Fan LQ, Zhao Q, Song ZC and Wang D: Proteomic identification and comparison of differentiation-related proteins in gastric carcinoma cell lines. Zhonghua Zhong Liu Za Zhi. 32:179–184. 2010.(In Chinese). PubMed/NCBI
13	Li Y, Tan BB, Zhao Q, Fan LQ, Liu Y and Wang D: Regulatory mechanism of ZNF139 in multi-drug resistance of gastric cancer cells. Mol Biol Rep. 41:3603–3610. 2014. View Article : Google Scholar : PubMed/NCBI
14	Roy MJ, Vom A, Czabotar PE and Lessene G: Cell death and the mitochondria: Therapeutic targeting of the BCL-2 family-driven pathway. Br J Pharmacol. 171:1973–1987. 2014. View Article : Google Scholar : PubMed/NCBI
15	Rubio N, Garcia-Segura LM and Arevalo MA: Survivin prevents apoptosis by binding to caspase-3 in astrocytes infected with the BeAn strain of Theiler's murine encephalomyelitis virus. J Neurovirol. 18:354–363. 2012. View Article : Google Scholar : PubMed/NCBI