Effects of ZNF139 on gastric cancer cells and mice with gastric tumors

Nie,Hong‑Feng; Li,Yong; Li,Zhen‑Xing; Mu,Ji‑Xing; Wang,Jin‑Sheng

doi:10.3892/ol.2016.4982

Effects of ZNF139 on gastric cancer cells and mice with gastric tumors

Authors:
- Hong‑Feng Nie
- Yong Li
- Zhen‑Xing Li
- Ji‑Xing Mu
- Jin‑Sheng Wang
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Affiliations: Department of General Surgery, The First Affiliated Hospital, Xingtai Medical College, Xingtai, Hebei 054001, P.R. China, Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of General Surgery, Hebei United University Affiliated Hospital, Tangshan, Hebei 063000, P.R. China
Published online on: August 10, 2016 https://doi.org/10.3892/ol.2016.4982
Pages: 2550-2554
Copyright: © Nie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer (GC) is the fourth most common type of cancer, worldwide. The major molecular factors associated with the pathogenesis of GC remain unclear. Previous studies found that zinc finger proteins are highly abundant in human eukaryotes and tissues, and play an important role in maintaining normal cellular functions and have an association with tumor initiation. In the current study, interference technology was used to silence the ZNF139 protein, a zinc finger protein that was previously found to be closely associated with GC. The results showed that cell viability and proliferation were inhibited in the Znf139‑knockdown of GC cells. Additional study found that the expression levels of B cell lymphoma‑2 (Bcl‑2) and survivin messenger RNAs and proteins were downregulated in Znf139‑silenced cells, indicating that cells expression Znf139 are able to induce the growth of tumor cells by mediating the apoptosis pathway. Further in vivo experiments showed that Znf139 knockdown downregulated the expression levels of Bcl‑2 and survivin in mice. Overall, the in vitro and in vivo findings of the present study indicate that ZNF139 may be actively involved in the progression of GC.

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