Evaluation by quantitative image analysis of anticancer drug activity on multicellular spheroids grown in 3D matrices

Gomes,Aurélie; Russo,Adrien; Vidal,Guillaume; Demange,Elise; Pannetier,Pauline; Souguir,Zied; Lagarde,Jean‑Michel; Ducommun,Bernard; Lobjois,Valérie

doi:10.3892/ol.2016.5221

December-2016 Volume 12 Issue 6

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December-2016 Volume 12 Issue 6

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Article Open Access

Evaluation by quantitative image analysis of anticancer drug activity on multicellular spheroids grown in 3D matrices

Authors:
- Aurélie Gomes
- Adrien Russo
- Guillaume Vidal
- Elise Demange
- Pauline Pannetier
- Zied Souguir
- Jean‑Michel Lagarde
- Bernard Ducommun
- Valérie Lobjois
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Affiliations: Institut des Technologies Avancées en sciences du Vivant, Centre National de la Recherche Scientifique, Université Paul Sabatier, Université de Toulouse, 31106 Toulouse, France, CELENYS, 76000 Rouen, France, IMACTIV‑3D, Centre Pierre Potier, 31106 Toulouse, France

Copyright: © Gomes et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 4371-4376
|
Published online on: October 4, 2016

https://doi.org/10.3892/ol.2016.5221
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Abstract

Pharmacological evaluation of anticancer drugs using 3D in vitro models provides invaluable information for predicting in vivo activity. Artificial matrices are currently available that scale up and increase the power of such 3D models. The aim of the present study was to propose an efficient and robust imaging and analysis pipeline to assess with quantitative parameters the efficacy of a particular cytotoxic drug. HCT116 colorectal adenocarcinoma tumor cell multispheres were grown in a 3D physiological hyaluronic acid matrix. 3D microscopy was performed with structured illumination, whereas image processing and feature extraction were performed with custom analysis tools. This procedure makes it possible to automatically detect spheres in a large volume of matrix in 96‑well plates. It was used to evaluate drug efficacy in HCT116 spheres treated with different concentrations of topotecan, a DNA topoisomerase inhibitor. Following automatic detection and quantification, changes in cluster size distribution with a topotecan concentration‑dependent increase of small clusters according to drug cytotoxicity were observed. Quantitative image analysis is thus an effective means to evaluate and quantify the cytotoxic and cytostatic activities of anticancer drugs on 3D multicellular models grown in a physiological matrix.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Evaluation by quantitative image analysis of anticancer drug activity on multicellular spheroids grown in 3D matrices

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