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Article Open Access

Upregulation of microRNA-181b inhibits CCL18-induced breast cancer cell metastasis and invasion via the NF-κB signaling pathway

Retraction in: /10.3892/ol.2024.14401
  • Authors:
    • Lei Wang
    • Yu‑Xia Wang
    • Li‑Ping Chen
    • Ming‑Li Ji
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China, Department of Pathophysiology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Physiology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4411-4418
    |
    Published online on: October 5, 2016
       https://doi.org/10.3892/ol.2016.5230
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Abstract

The purpose of the present study was to investigate the effects of upregulating microRNA (miR)-181b expression in tumor-associated macrophages regarding breast cancer cell metastasis and to identify the target gene. Ectopic miR‑181b was transfected into MDA‑MB‑231 and MCF‑7 breast cancer cell lines with or without chemokine ligand 18 (CCL18) stimulation. Cell proliferation, migration/invasion and apoptosis rate were investigated. The binding effects of miR‑181b to the 3'‑untranslated region (UTR) of the nuclear factor (NF)‑κB gene were detected with the dual luciferase reporter system. Immunofluorescent staining of the NF‑κB key component P65 was performed. The messenger (m) RNA and protein expression of NF‑κB induced by CCL18 with or without miR‑181b stimulation was evaluated with reverse transcription‑quantitative polymerase chain reaction and western blot analysis. When compared with the CCL18‑stimulated group, miR‑181b mimic-transfected cells exhibited significantly inhibited proliferation and migration, with an increased cell apoptosis percentage in a dose‑dependent manner. Furthermore, the luciferase activity was reduced for cells with NF‑κB 3'‑UTR wild‑type that were co‑transfected with miR‑181b mimics. Immunofluorescent staining of NF‑κB demonstrably weakened the P65 signal in stimulated miR‑181b mimic cells when compared with parental and CCL18‑treated cells. The increased expression level of NF‑κB induced by CCL18 in MDA‑MB‑231 and MCF‑7 cells was suppressed by miR‑181b mimics. Overexpression of miR‑181b suppressed cell survival rate and migration. This overexpression may achieve this goal by regulating the NF‑κB pathway in breast cancer cells. Our study demonstrated a potential therapeutic application of miR‑181b in the treatment of breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Wang L, Wang YX, Chen LP and Ji ML: Upregulation of microRNA-181b inhibits CCL18-induced breast cancer cell metastasis and invasion via the NF-κB signaling pathway Retraction in /10.3892/ol.2024.14401. Oncol Lett 12: 4411-4418, 2016.
APA
Wang, L., Wang, Y., Chen, L., & Ji, M. (2016). Upregulation of microRNA-181b inhibits CCL18-induced breast cancer cell metastasis and invasion via the NF-κB signaling pathway Retraction in /10.3892/ol.2024.14401. Oncology Letters, 12, 4411-4418. https://doi.org/10.3892/ol.2016.5230
MLA
Wang, L., Wang, Y., Chen, L., Ji, M."Upregulation of microRNA-181b inhibits CCL18-induced breast cancer cell metastasis and invasion via the NF-κB signaling pathway Retraction in /10.3892/ol.2024.14401". Oncology Letters 12.6 (2016): 4411-4418.
Chicago
Wang, L., Wang, Y., Chen, L., Ji, M."Upregulation of microRNA-181b inhibits CCL18-induced breast cancer cell metastasis and invasion via the NF-κB signaling pathway Retraction in /10.3892/ol.2024.14401". Oncology Letters 12, no. 6 (2016): 4411-4418. https://doi.org/10.3892/ol.2016.5230
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Wang YX, Chen LP and Ji ML: Upregulation of microRNA-181b inhibits CCL18-induced breast cancer cell metastasis and invasion via the NF-κB signaling pathway Retraction in /10.3892/ol.2024.14401. Oncol Lett 12: 4411-4418, 2016.
APA
Wang, L., Wang, Y., Chen, L., & Ji, M. (2016). Upregulation of microRNA-181b inhibits CCL18-induced breast cancer cell metastasis and invasion via the NF-κB signaling pathway Retraction in /10.3892/ol.2024.14401. Oncology Letters, 12, 4411-4418. https://doi.org/10.3892/ol.2016.5230
MLA
Wang, L., Wang, Y., Chen, L., Ji, M."Upregulation of microRNA-181b inhibits CCL18-induced breast cancer cell metastasis and invasion via the NF-κB signaling pathway Retraction in /10.3892/ol.2024.14401". Oncology Letters 12.6 (2016): 4411-4418.
Chicago
Wang, L., Wang, Y., Chen, L., Ji, M."Upregulation of microRNA-181b inhibits CCL18-induced breast cancer cell metastasis and invasion via the NF-κB signaling pathway Retraction in /10.3892/ol.2024.14401". Oncology Letters 12, no. 6 (2016): 4411-4418. https://doi.org/10.3892/ol.2016.5230
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