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Article

Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

  • Authors:
    • Chunyan Xue
    • Yueqin Chen
    • Dan‑Ning Hu
    • Codrin Iacob
    • Chengwei Lu
    • Zhenping Huang
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu 210002, P.R. China, Tissue Culture Center, The New York Eye and Ear Infirmary of Mount Sinai, New York, NY 10003, USA, Department of Pathology, The New York Eye and Ear Infirmary of Mount Sinai, New York, NY 10003, USA, Department of Ophthalmology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 4813-4820
    |
    Published online on: October 13, 2016
       https://doi.org/10.3892/ol.2016.5251
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Abstract

Uveal melanoma is the most common intraocular malignant tumor in adults. Chrysin is a flavonoid present in honey, propolis, various plants and herbs. In the present study, the cytotoxic effects of chrysin were investigated on human uveal melanoma cell lines (M17 and SP6.5) and associated signaling pathways, and a comparison to the effects on normal ocular cells [scleral fibroblasts and retinal pigment epithelial (RPE) cells] was performed. The effects of chrysin on cell viability were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was determined by using terminal deoxynucleotidyl transferase mediated dUTP nick end‑labeling assay. Mitochondrial permeability was determined by JC‑1 fluorescein analysis. Cytosol cytochrome c levels, and the activities of caspase-3, ‑8 and ‑9 were measured by enzyme‑linked immunosorbent assay or colorimetric assay. Chrysin reduced the viability of cultured human melanoma cells in a dose‑dependent manner (0, 10, 30 and 100 µM) with IC50 at 28.3 and 35.8 µM in SP6.5 and M17 cell lines, respectively. Chrysin at 30‑100 µM levels selectively reduced the viability of melanoma cells without affecting the viability of scleral fibroblasts and RPE cells. Chrysin increased mitochondrial permeability, the levels of cytosol cytochrome c, and caspase‑9 and ‑3 activities, but not capase‑8 activity in uveal melanoma cells. The results of the present study indicate that chrysin induces apoptosis of human uveal melanoma cells via the mitochondrial signaling pathway and suggest that chrysin may be a promising agent in the treatment of uveal melanoma.
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1 

Hu DN, Yu GP, McCormick SA, Schneider S and Finger PT: Population-based incidence of uveal melanoma in various races and ethnic groups. Am J Ophthalmol. 140:612–617. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Kujala E, Mäkitie T and Kivelä T: Very long-term prognosis of patients with malignant uveal melanoma. Invest Ophthalmol Vis Sci. 44:4651–4659. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Augsburger JJ, Corrêa ZM and Shaikh AH: Effectiveness of treatments for metastatic uveal melanoma. Am J Ophthalmol. 148:119–127. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Monasterio A, Urdaci MC, Pinchuk IV, López-Moratalla N and Martínez-Irujo JJ: Flavonoids induce apoptosis in human leukemia U937 cells through caspase- and caspase-calpain-dependent pathways. Nutr Cancer. 50:90–100. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Samarghandian S, Afshari JT and Davoodi S: Chrysin reduces proliferation and induces apoptosis in the human prostate cancer cell line pc-3. Clinics (Sao Paulo). 66:1073–1079. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Cho H, Yun CW, Park WK, Kong JY, Kim KS, Park Y, Lee S and Kim BK: Modulation of the activity of pro-inflammatory enzymes, COX-2 and iNOS, by chrysin derivatives. Pharmacol Res. 49:37–43. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Woodman OL and Chan ECh: Vascular and anti-oxidant actions of flavonols and flavones. Clin Exp Pharmacol Physiol. 31:786–790. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Khoo BY, Chua SL and Balaram P: Apoptotic effects of chrysin in human cancer cell lines. Int J Mol Sci. 11:2188–2199. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Pichichero E, Cicconi R, Mattei M and Canini A: Chrysin-induced apoptosis is mediated through p38 and Bax activation in B16-F1 and A375 melanoma cells. Int J Oncol. 38:473–483. 2011.PubMed/NCBI

10 

Pichichero E, Cicconi R, Mattei M, Muzi MG and Canini A: Acacia honey and chrysin reduce proliferation of melanoma cells through alterations in cell cycle progression. Int J Oncol. 37:973–981. 2010.PubMed/NCBI

11 

Yang B, Huang J, Xiang T, Yin X, Luo X, Huang J, Luo F, Li H, Li H and Ren G: Chrysin inhibits metastatic potential of human triple-negative breast cancer cells by modulating matrix metalloproteinase-10, epithelial to mesenchymal transition, and PI3K/Akt signaling pathway. J Appl Toxicol. 34:105–112. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Samarghandian S, Nezhad MA and Mohammadi G: Role of caspases, Bax and Bcl-2 in chrysin-induced apoptosis in the A549 human lung adenocarcinoma epithelial cells. Anticancer Agents Med Chem. 14:901–909. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Lee SJ, Yoon JH and Song KS: Chrysin inhibited stem cell factor (SCF)/c-Kit complex-induced cell proliferation in human myeloid leukemia cells. Biochem Pharmacol. 74:215–225. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Sak K: Cytotoxicity of dietary flavonoids on different human cancer types. Pharmacogn Rev. 8:122–146. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Yu XM, Phan T, Patel PN, Jaskula-Sztul R and Chen H: Chrysin activates Notch1 signaling and suppresses tumor growth of anaplastic thyroid carcinoma in vitro and in vivo. Cancer. 119:774–781. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Shao JJ, Zhang AP, Qin W, Zheng L, Zhu YF and Chen X: AMP-activated protein kinase (AMPK) activation is involved in chrysin-induced growth inhibition and apoptosis in cultured A549 lung cancer cells. Biochem Biophys Res Commun. 423:448–453. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Phan T, Yu XM, Kunnimalaiyaan M and Chen H: Antiproliferative effect of chrysin on anaplastic thyroid cancer. J Surg Res. 170:84–88. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Pilátová M, Stupáková V, Varinská L, Sarisský M, Mirossay L, Mirossay A, Gál P, Kraus V, Dianisková K and Mojzis J: Effect of selected flavones on cancer and endothelial cells. Gen Physiol Biophys. 29:134–143. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Hong TB, Rahumatullah A, Yogarajah T, Ahmad M and Yin KB: Potential effects of chrysin on MDA-MB-231 cells. Int J Mol Sci. 11:1057–1069. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Weng MS, Ho YS and Lin JK: Chrysin induces G1 phase cell cycle arrest in C6 glioma cells through inducing p21Waf1/Cip1 expression: Involvement of p38 mitogen-activated protein kinase. Biochem Pharmacol. 69:1815–1827. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Woo KJ, Jeong YJ, Park JW and Kwon TK: Chrysin-induced apoptosis is mediated through caspase activation and Akt inactivation in U937 leukemia cells. Biochem Biophys Res Commun. 325:1215–1222. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Zhang T, Chen X, Qu L, Wu J, Cui R and Zhao Y: Chrysin and its phosphate ester inhibit cell proliferation and induce apoptosis in Hela cells. Bioorg Med Chem. 12:6097–6105. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Hu DN, McCormick SA, Ritch R and Pelton-Henrion K: Studies of human uveal melanocytes in vitro: Isolation, purification and cultivation of human uveal melanocytes. Invest Ophthalmol Vis Sci. 34:2210–2219. 1993.PubMed/NCBI

24 

Soulieres D, Rousseau A, Deschenes J, Tremblay M, Tardif M and Pelletier G: Characterization of gangliosides in human uveal melanoma cells. Int J Cancer. 49:498–503. 1991. View Article : Google Scholar : PubMed/NCBI

25 

Lu C, Song E, Hu DN, Chen M, Xue C, Rosen R and McCormick SA: Curcumin induces cell death in human uveal melanoma cells through mitochondrial pathway. Curr Eye Res. 35:352–360. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Parthasarathy G and Philipp MT: The MEK/ERK pathway is the primary conduit for Borrelia burgdorferi-induced inflammation and P53-mediated apoptosis in oligodendrocytes. Apoptosis. 19:76–89. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Turgeman T, Kakongi N, Schneider A, Vinokur Y, Teper-Bamnolker P, Carmeli S, Levy M, Skory CD, Lichter A and Eshel D: Induction of Rhizopus oryzae germination under starvation using host metabolites increases spore susceptibility to heat stress. Phytopathology. 104:240–247. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Cossarizza A, Baccarani-Contri M, Kalashnikova G and Franceschi C: A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolcarbocyanine iodide (JC-1). Biochem Biophys Res Commun. 197:40–45. 1993. View Article : Google Scholar : PubMed/NCBI

29 

Gilchrest BA, Eller MS, Geller AC and Yaar M: The pathogenesis of melanoma induced by ultraviolet radiation. N Engl J Med. 340:1341–1348. 1999. View Article : Google Scholar : PubMed/NCBI

30 

Bergman L, Seregard S, Nilsson B, Ringborg U, Lundell G and Ragnarsson-Olding B: Incidence of uveal melanoma in Sweden from 1960 to 1998. Invest Ophthalmol Vis Sci. 43:2579–2583. 2002.PubMed/NCBI

31 

Yu GP, Hu DN and McCormick SA: Latitude and incidence of ocular melanoma. Photochem Photobiol. 82:1621–1626. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Spendlove HE, Damato BE, Humphreys J, Barker KT, Hiscott PS and Houlston RS: BRAF mutations are detectable in conjunctival but not uveal melanomas. Melanoma Res. 14:449–452. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Vajdic CM, Hutchins AM, Kricker A, Aitken JF, Armstrong BK, Hayward NK and Armes JE: Chromosomal gains and losses in ocular melanoma detected by comparative genomic hybridization in an Australian population-based study. Cancer Genet Cytogenet. 144:12–17. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Hu DN, Yu G, McCormick SA and Finger PT: Population-based incidence of conjunctival melanoma in various races and ethnic groups and comparison with other melanomas. Am J Ophthalmol. 145:418–423. 2008. View Article : Google Scholar : PubMed/NCBI

35 

McConkey DJ: Biochemical determinants of apoptosis and necrosis. Toxicol Lett. 99:157–168. 1998. View Article : Google Scholar : PubMed/NCBI

36 

Kroemer G, Dallaporta B and Resche-Rigon M: The mitochondrial death/life regulator in apoptosis and necrosis. Annu Rev Physiol. 60:619–642. 1998. View Article : Google Scholar : PubMed/NCBI

37 

Chen X, Wang J, Shen H, Lu J, Li C, Hu DN, Dong XD, Yan D and Tu L: Epigenetics, microRNAs, and carcinogenesis: Functional role of microRNA-137 in uveal melanoma. Invest Ophthalmol Vis Sci. 52:1193–1199. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xue C, Chen Y, Hu DN, Iacob C, Lu C and Huang Z: Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis. Oncol Lett 12: 4813-4820, 2016.
APA
Xue, C., Chen, Y., Hu, D., Iacob, C., Lu, C., & Huang, Z. (2016). Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis. Oncology Letters, 12, 4813-4820. https://doi.org/10.3892/ol.2016.5251
MLA
Xue, C., Chen, Y., Hu, D., Iacob, C., Lu, C., Huang, Z."Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis". Oncology Letters 12.6 (2016): 4813-4820.
Chicago
Xue, C., Chen, Y., Hu, D., Iacob, C., Lu, C., Huang, Z."Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis". Oncology Letters 12, no. 6 (2016): 4813-4820. https://doi.org/10.3892/ol.2016.5251
Copy and paste a formatted citation
x
Spandidos Publications style
Xue C, Chen Y, Hu DN, Iacob C, Lu C and Huang Z: Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis. Oncol Lett 12: 4813-4820, 2016.
APA
Xue, C., Chen, Y., Hu, D., Iacob, C., Lu, C., & Huang, Z. (2016). Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis. Oncology Letters, 12, 4813-4820. https://doi.org/10.3892/ol.2016.5251
MLA
Xue, C., Chen, Y., Hu, D., Iacob, C., Lu, C., Huang, Z."Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis". Oncology Letters 12.6 (2016): 4813-4820.
Chicago
Xue, C., Chen, Y., Hu, D., Iacob, C., Lu, C., Huang, Z."Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis". Oncology Letters 12, no. 6 (2016): 4813-4820. https://doi.org/10.3892/ol.2016.5251
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