Activator protein 1 promotes gemcitabine‑induced apoptosis in pancreatic cancer by upregulating its downstream target Bim

  • Authors:
    • Xiaoxia Ren
    • Wenjing Zhao
    • Yongxing Du
    • Taiping Zhang
    • Lei You
    • Yupei Zhao
  • View Affiliations

  • Published online on: October 19, 2016     https://doi.org/10.3892/ol.2016.5294
  • Pages: 4732-4738
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Abstract

Gemcitabine is a commonly used chemotherapy drug in pancreatic cancer. The function of activator protein 1 (AP‑1) is cell‑specific, and its function depends on the expression of other complex members. In the present study, we added gemcitabine to the media of Panc‑1 and SW1990 cells at clinically achieved concentrations (10 µM). Compared with constitutive c‑Fos expression, c‑Jun expression increased in a dose‑dependent manner upon gemcitabine treatment. c‑Jun overexpression increased gemcitabine‑induced apoptosis through Bim activation, while cell apoptosis and Bim expression decreased following c‑Jun knockdown. Furthermore, gemcitabine‑induced apoptosis and Bim levels decreased when c‑Jun phosphorylation was blocked by SP600125. Our findings suggest that c‑Jun, which is a member of the AP‑1 complex, functions in gemcitabine‑induced apoptosis by regulating its downstream target Bim in pancreatic cancer cells.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ren X, Zhao W, Du Y, Zhang T, You L and Zhao Y: Activator protein 1 promotes gemcitabine‑induced apoptosis in pancreatic cancer by upregulating its downstream target Bim. Oncol Lett 12: 4732-4738, 2016
APA
Ren, X., Zhao, W., Du, Y., Zhang, T., You, L., & Zhao, Y. (2016). Activator protein 1 promotes gemcitabine‑induced apoptosis in pancreatic cancer by upregulating its downstream target Bim. Oncology Letters, 12, 4732-4738. https://doi.org/10.3892/ol.2016.5294
MLA
Ren, X., Zhao, W., Du, Y., Zhang, T., You, L., Zhao, Y."Activator protein 1 promotes gemcitabine‑induced apoptosis in pancreatic cancer by upregulating its downstream target Bim". Oncology Letters 12.6 (2016): 4732-4738.
Chicago
Ren, X., Zhao, W., Du, Y., Zhang, T., You, L., Zhao, Y."Activator protein 1 promotes gemcitabine‑induced apoptosis in pancreatic cancer by upregulating its downstream target Bim". Oncology Letters 12, no. 6 (2016): 4732-4738. https://doi.org/10.3892/ol.2016.5294