Analysis of transcription profile to reveal altered signaling pathways following the overexpression of human desumoylating isopeptidase 2 in pancreatic cancer cells

Fu,Yu‑Yin; Kang,Yu‑Huan; Shen,Cong‑Cong; Wang,Rui‑Xue; Yu,Lin; Li,Xin‑Yue; Cui,Dan‑Dan; Yang,Jin‑Liang; Yao,Yu‑Qin; Gou,Lan‑Tu

doi:10.3892/ol.2016.5298

Analysis of transcription profile to reveal altered signaling pathways following the overexpression of human desumoylating isopeptidase 2 in pancreatic cancer cells

Authors:
- Yu‑Yin Fu
- Yu‑Huan Kang
- Cong‑Cong Shen
- Rui‑Xue Wang
- Lin Yu
- Xin‑Yue Li
- Dan‑Dan Cui
- Jin‑Liang Yang
- Yu‑Qin Yao
- Lan‑Tu Gou
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Affiliations: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan 610041, P.R. China, Department of Medical Oncology, The Fifth People's Hospital of Chengdu, Chengdu, Sichuan 610041, P.R. China, Research Center for Public Health and Preventive Medicine, West China School of Public Health/No. 4 West China Teaching Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
Published online on: October 19, 2016 https://doi.org/10.3892/ol.2016.5298
Pages: 4677-4684

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Abstract

Human desumoylating isopeptidase 2 (DESI‑2) is a member of the DESI family and contains a conserved PPPDE1 domain. Previous studies have demonstrated that DESI‑2 overexpression may induce cell apoptosis. In the present study, differentially expressed genes were analyzed using a transcription microarray in DESI‑2 overexpressing PANC‑1 pancreatic cancer cells. A total of 45,033 genes were examined by microarray, which identified 1,766 upregulated and 1,643 downregulated genes. A series of altered signaling pathways were analyzed, in which certain essential signaling factors, including retinoid X receptor (RXR), BH3 interacting‑domain death agonist, Ras homolog gene family member A (RhoA) and Rho‑associated protein kinase, were further investigated at the protein level. The release of cytochrome c and the activation of caspase‑3 were also detected by western blot analysis. Immunohistochemistry further revealed the expression features of RXR and RhoA in pancreatic ductal adenocarcinoma tissues with various DESI‑2 expression levels. The results serve as a valuable reference for the further elucidation of the functions of DESI‑2 in pancreatic cancer.

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