Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
December-2016 Volume 12 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2016 Volume 12 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Programmed cell death 1 correlates with the occurrence and development of MG63 osteosarcoma

  • Authors:
    • Fuyou Zhao
    • Qiong Wu
    • Xiusong Dai
    • Yumei Li
    • Huaiyong Gan
    • Ri Wang
    • Jie Lv
    • Yuqing Chen
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233000, P.R. China, Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233000, P.R. China, Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233000, P.R. China, Department of Microbiology, Bengbu Medical College, Bengbu, Anhui 233000, P.R. China, Department of Respiratory Diseases, The Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233000, P.R. China
  • Pages: 5199-5204
    |
    Published online on: October 24, 2016
       https://doi.org/10.3892/ol.2016.5311
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to investigate the effect of programmed cell death 1 (PD‑1) on osteosarcoma (OD) stem cells and T cells, and to determine their correlation. OS stem cells were sorted and identified from OS MG63 cells. Flow cytometry was used to detect the PD‑1 expression of the OS tumor stem cell membrane surface. The expression of PD‑1 mRNA was detected by reverse transcription‑polymerase chain reaction (RT‑PCR). MTT was used to detect the effect of PD‑1 signals on T‑cell proliferation. The results indicated that the cancer cells (cultured in DMEM medium containing 10% fetal bovine serum) exhibited clear proliferation within 1 week of cell culture, which showed their strong proliferation and aggressive ability. The formation of tumor cell spheres was dependent on the support of serum nutrition. The proliferation of MG63 cells in the serum culture medium was significantly higher than the number of OS cell spheres in serum‑free suspension culture (P<0.05). Pluripotent stem cells in cancer cell spheres exhibited significantly higher cluster of differentiation 133 expression compared with the MG63 cells. The PD‑1 expression levels of the cancer cell spheres was significantly increased compared with the MG63 cells, which is consistent with the results of the RT‑PCR. In conclusion, the MG63 cell line possesses the features of OS stem cells. The MG63 cell line can express the certain cancer‑associated cell markers. The expression of PD‑1 in spheres was also increased significantly compared to the MG63 cells, which can reduce the immune function of patients and may be closely associated with the occurrence and development of tumors.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Bai X, Meng H, Ma L and Guo A: Inhibitory effects of evodiamine human osteosarcoma cell proliferation and apoptosis. Oncol Lett. 9:801–805. 2015.PubMed/NCBI

2 

Fleuren ED, Versleijen-Jonkers YM, Boerman OC and van der Graaf WT: Targeting receptor tyrosine kinases in osteosarcoma and Ewing sarcoma: Current hurdles and future perspectives. Biochim Biophys Acta. 1845:266–276. 2014.PubMed/NCBI

3 

Botter SM, Neri D and Fuchs B: Recent advances in osteosarcoma. Curr Opin Pharmacol. 16:15–23. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Siewe B, Wallace J, Rygielski S, Stapleton JT, Martin J, Deeks SG and Landay A: Regulatory B cells inhibit cytotoxic T lymphocyte (CTL) activity and elimination of infected CD4 T cells after in vitro reactivation of HIV latent reservoirs. PLoS One. 9:e929342014. View Article : Google Scholar : PubMed/NCBI

5 

Xu P, Chen YJ, Chen H, Zhu XY, Song HF, Cao LJ and Wang XF: The expression of programmed death-1 in circulating CD4+ and CD8+ T cells during hepatitis B virus infection progression and its correlation with clinical baseline characteristics. Gut Liver. 8:186–195. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Philip NH, Dillon CP, Snyder AG, Fitzgerald P, Wynosky-Dolfi MA, Zwack EE, Hu B, Fitzgerald L, Mauldin EA, Copenhaver AM, et al: Caspase-8 mediates caspase-1 processing and innate immune defense in response to bacterial blockade of NF-kB and MAPK signaling. Proc Natl Acad Sci USA. 11:7385–7590. 2014. View Article : Google Scholar

7 

Pedoeem A, Azoulay-Alfaguter I, Strazza M, Silverman GJ and Mor A: Programmed death-1 pathway in cancer and autoimmunity. Clin Immunol. 153:145–152. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Notaro A, Sabella S, Pellerito O, Di Fiore R, De Blasio A, Calvaruso G and Giuliano M: Involvement of PAR-4 in cannabinoid-dependent sensitization of osteosarcoma cells to TRAIL-induced apoptosis. Int J Biol Sci. 10:466–478. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Oba J, Nakahara T, Abe T, Hagihara A, Moroi Y and Furue M: Expression of programmed death receptor ligand 1 in melanoma may indicate tumor progression and poor patient survival. J Am Acad Dermatol. 70:954–956. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Mittendorf EA, Philips AV, Meric-Bernstam F, Qiao N, Wu Y, Harrington S, Su X, Wang Y, Gonzalez-Angulo AM, Akcakanat A, et al: PD-L1 expression in triple-negative breast cancer. Cancer Immunol Res. 2:361–370. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Joseph RW, Parasramka M, Eckel-Passow JE, Seri D, Wu K, Jiang L, Kalari K, Thompson RJ, Huu Ho T, Castle EP, et al: Inverse association between programmed death ligand 1 and genes in the VEGF pathway in primary clear cell renal cell carcinoma. Cancer Immunol Res. 1:378–385. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Macfarlane AW IV, Jillab M, Plimack ER, Hudes GR, Uzzo RG, Litwin S, Dulaimi E, Al-Saleem T and Campbell KS: PD-1 expression on peripheral blood cells increases with stage in renal cell carcinoma patients and is rapidly reduced after surgical tumor resection. Cancer Immunol Res. 2:320–331. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Flies DB, Han X, Higuchi T, Zheng L, Sun J, Ye JJ and Chen L: Coinhibitory receptor PD-1H preferentially suppresses CD4+ T cell-mediated immunity. J Clin Invest. 124:1966–1975. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Barker CA and Postow MA: Combinations of radiation therapy and immunotherapy for melanoma: A review of clinical outcomes. Int J Radiat Oncol Biol Phys. 88:986–997. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Tosti G, Cocorocchio E and Pennacchioli E: Anti-cytotoxic T lymphocyte antigen-4 antibodies in melanoma. Clin Cosmet Investig Dermatol. 6:245–256. 2013.PubMed/NCBI

16 

Feng D, Yang X, Li S, Liu T, Wu Z, Song Y, Wang J, Gao W, Huang Q, Huang W, et al: Cytotoxic T-lymphocyte antigen-4 genetic variants and risk of Ewing's sarcoma. Genet Test Mol Biomarkers. 17:458–463. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Liu Y, He Z, Feng D, Shi G, Gao R, Wu X, Song W and Yuan W: Cytotoxic T-lymphocyte antigen-4 polymorphisms and susceptibility to osteosarcoma. DNA Cell Biol. 30:1051–1055. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Wang W, Wang J, Song H, Liu J, Song B and Cao X: Cytotoxic T-lymphocyte antigen-4+ 49G/A polymorphism is associated with increased risk of osteosarcoma. Genet Test Mol Biomarkers. 15:503–506. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Yang S, Wang C, Zhou Y, Sun G, Zhu D and Gao S: Cytotoxic T-lymphocyte antigen-4 polymorphisms and susceptibility to Ewing's sarcoma. Genet Test Mol Biomarkers. 16:1236–1240. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Shang Y, Li Z, Li H, Xia H and Lin Z: TIM-3 expression in human osteosarcoma: Correlation with the expression of epithelial-mesenchymal transition-specific biomarkers. Oncol Lett. 6:490–494. 2013.PubMed/NCBI

21 

Ramsay AG: Immune checkpoint blockade immunotherapy to activate anti-tumour T-cell immunity. Br J Haematol. 162:313–325. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Ott PA, Hodi FS and Robert C: CTLA-4 and PD-1/PDL1 blockade: New immunotherapeutic modalities with durable clinical benefit in melanoma patients. Clin Cancer Res. 19:5300–5309. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Okazaki T, Chikuma S, Iwai Y, Fagarasan S and Honjo T: A rheostat for immune responses: The unique properties of PD-1 and their advantages for clinical application. Nat Immunol. 14:1212–1218. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Pan XC, Li L, Mao JJ, Yao W, Zheng JN, Liu M and Fu JJ: Synergistic effects of soluble PD-1 and IL-21 on antitumor immunity against H22 murine hepatocellular carcinoma. Oncol Lett. 5:90–96. 2013.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhao F, Wu Q, Dai X, Li Y, Gan H, Wang R, Lv J and Chen Y: Programmed cell death 1 correlates with the occurrence and development of MG63 osteosarcoma. Oncol Lett 12: 5199-5204, 2016.
APA
Zhao, F., Wu, Q., Dai, X., Li, Y., Gan, H., Wang, R. ... Chen, Y. (2016). Programmed cell death 1 correlates with the occurrence and development of MG63 osteosarcoma. Oncology Letters, 12, 5199-5204. https://doi.org/10.3892/ol.2016.5311
MLA
Zhao, F., Wu, Q., Dai, X., Li, Y., Gan, H., Wang, R., Lv, J., Chen, Y."Programmed cell death 1 correlates with the occurrence and development of MG63 osteosarcoma". Oncology Letters 12.6 (2016): 5199-5204.
Chicago
Zhao, F., Wu, Q., Dai, X., Li, Y., Gan, H., Wang, R., Lv, J., Chen, Y."Programmed cell death 1 correlates with the occurrence and development of MG63 osteosarcoma". Oncology Letters 12, no. 6 (2016): 5199-5204. https://doi.org/10.3892/ol.2016.5311
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao F, Wu Q, Dai X, Li Y, Gan H, Wang R, Lv J and Chen Y: Programmed cell death 1 correlates with the occurrence and development of MG63 osteosarcoma. Oncol Lett 12: 5199-5204, 2016.
APA
Zhao, F., Wu, Q., Dai, X., Li, Y., Gan, H., Wang, R. ... Chen, Y. (2016). Programmed cell death 1 correlates with the occurrence and development of MG63 osteosarcoma. Oncology Letters, 12, 5199-5204. https://doi.org/10.3892/ol.2016.5311
MLA
Zhao, F., Wu, Q., Dai, X., Li, Y., Gan, H., Wang, R., Lv, J., Chen, Y."Programmed cell death 1 correlates with the occurrence and development of MG63 osteosarcoma". Oncology Letters 12.6 (2016): 5199-5204.
Chicago
Zhao, F., Wu, Q., Dai, X., Li, Y., Gan, H., Wang, R., Lv, J., Chen, Y."Programmed cell death 1 correlates with the occurrence and development of MG63 osteosarcoma". Oncology Letters 12, no. 6 (2016): 5199-5204. https://doi.org/10.3892/ol.2016.5311
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team