Dysregulation of MALAT1 and miR-619‑5p as a prognostic indicator in advanced colorectal carcinoma

Qiu,Gu; Zhang,Xiu‑Bing; Zhang,Shu‑Qing; Liu,Pei‑Long; Wu,Wei; Zhang,Jin‑Ye; Dai,Shi‑Rong

doi:10.3892/ol.2016.5312

Dysregulation of MALAT1 and miR-619‑5p as a prognostic indicator in advanced colorectal carcinoma

Authors:
- Gu Qiu
- Xiu‑Bing Zhang
- Shu‑Qing Zhang
- Pei‑Long Liu
- Wei Wu
- Jin‑Ye Zhang
- Shi‑Rong Dai
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Affiliations: Department of Clinical Laboratories, Nantong Second People's Hospital, Nantong, Jiangsu 226002, P.R. China, Department of Internal Medicine‑Oncology, Nantong Second People's Hospital, Nantong, Jiangsu 226002, P.R. China, Department of Experimental Center, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Clinical Laboratories, Nantong Tumor Hospital, Nantong, Jiangsu 226018, P.R. China
Published online on: October 24, 2016 https://doi.org/10.3892/ol.2016.5312
Pages: 5036-5042

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Abstract

The present study aimed to detect the expression of metastasis associated lung adenocarcinoma transcript 1 (MALAT1) and microRNA (miR)-619-5p in colorectal carcinoma (CRC), and to evaluate the significance of MALAT1 and miR‑619‑5p expression in the clinical diagnosis and prognosis of CRC. Quantitative polymerase chain reaction was used to detect MALAT1 and miR‑619‑5p expression in 120 colorectal carcinoma and 120 adjacent normal tissue samples. The expression levels of MALAT1 and miR‑619‑5p were significantly different between colorectal carcinoma and adjacent normal tissues (P<0.05). MALAT1 exhibited an average 2.52‑fold increase in colorectal adenoma when compared with adjacent normal tissues, while miR‑619‑5p exhibited an average 5.79‑fold decrease in colorectal adenoma when compared with adjacent normal tissues. There was a significant difference between the MALAT1 expression in CRC tissues obtained from men and women (P=0.027), and in tumor-node-metastasis (TNM) stage II and stage III lesions (P=0.019). MALAT1 expression was associated with lymphovascular invasion (P=0.047) and perineural invasion (P=0.012). In addition, miR‑619‑5p expression was also significantly different between men and women (P=0.032), and between TNM stage II and stage III lesions (P=0.012). miR-619-5p expression was also associated with lymphovascular invasion (P=0.023) and perineural invasion (P=0.009). Patients with high expression of MALAT1 and low expression of miR‑619‑5p demonstrated significantly shorter disease-free survival (DFS) (P=0.002) and overall survival (OS) times (P=0.004) compared with patients with low MALAT1 expression and high miR‑619‑5p expression. Patients with perineural invasion demonstrated significantly shorter DFS (P=0.001) and OS times (P=0.003) compared with patients without perineural invasion. In addition, there was a negative correlation between MALAT1 expression and miR‑619‑5p expression (r=‑0.415, P=0.004) in CRC tissues. In conclusion, MALAT1 and miR‑619‑5p have potential for the molecular diagnosis of CRC patients, and combined assaying of MALAT1 and miR‑619‑5p may improve the accuracy of the diagnosis of CRC and act as a good prognostic indicator in CRC patients.

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