Detection value of tumor cells in cerebrospinal fluid in the diagnosis of meningeal metastasis from lung cancer by immuno-FISH technology

Lv,Yuan; Mu,Ning; Ma,Chunhua; Jiang,Rong; Wu,Qiaoli; Li,Jinduo; Wang,Bin; Sun,Liwei

doi:10.3892/ol.2016.5314

December-2016 Volume 12 Issue 6

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

December-2016 Volume 12 Issue 6

Full Size Image

Article Open Access

Detection value of tumor cells in cerebrospinal fluid in the diagnosis of meningeal metastasis from lung cancer by immuno-FISH technology

Authors:
- Yuan Lv
- Ning Mu
- Chunhua Ma
- Rong Jiang
- Qiaoli Wu
- Jinduo Li
- Bin Wang
- Liwei Sun
View Affiliations / Copyright

Affiliations: Department of Intervention, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Huanhu Hospital, Tianjin 300350, P.R. China

Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 5080-5084
|
Published online on: October 25, 2016

https://doi.org/10.3892/ol.2016.5314
Expand metrics +

Abstract

To investigate the detection value of tumor cells in cerebrospinal fluid (CSF) in the adjuvant diagnosis of meningeal metastasis from lung cancer by immunofluorescence in situ hybridization (immuno‑FISH) technology. The circulating tumor cells (CTCs) in the CSF of 16 patients with meningeal metastasis from lung cancer and 8 with non‑tumor diseases in the brain were detected using immuno‑FISH technology. The diagnosis of meningeal metastasis from lung cancer was based on neurological symptoms, enhanced magnetic resonance imaging (MRI) scans and CSF cytological examination. The number of CTCs in the patients with meningeal metastasis from lung cancer was significantly higher than those with non‑tumor diseases in the brain (P<0.01). The critical point of the maximum correct diagnostic index (Youden index) was regarded as the judging criterion for positive tumor cells in CSF according to the receiver operating characteristic curve. When there was one tumor cell in 7.5 ml CSF, the area under curve was 0.875 (95% confidence interval, 0.705~1.000). The diagnostic sensitivity, specificity, effectiveness, positive and negative predictive values were 75.0, 100.0, 83.3, 100.0 and 66.7%, respectively. There may be great clinical value in the detection of CTCs in CSF for the diagnosis of meningeal metastasis from lung cancer by immuno-FISH technology.

View Figures

View References

1	Leal T, Chang JE, Mehta M and Robins HI: Leptomeningeal metastasis: Challenges in diagnosis and treatment. Curr Cancer Ther Rev. 7:319–327. 2011. View Article : Google Scholar : PubMed/NCBI
2	Nagpal S, Riess J and Wakelee H: Treatment of leptomeningeal spread of NSCLC: A continuing challenge. Curr Treat Options Oncol. 13:491–504. 2012. View Article : Google Scholar : PubMed/NCBI
3	Yu Y, Chen Z, Dong J, Wei P, Hu R, Zhou C, Sun N, Luo M, Yang W, Yao R, et al: Folate receptor-positive circulating tumor cells as a novel diagnostic biomarker in non-small cell lung cancer. Transl Oncol. 6:697–702. 2013. View Article : Google Scholar : PubMed/NCBI
4	Igawa S, Gohda K, Fukui T, Ryuge S, Otani S, Masago A, Sato J, Murakami K, Maki S, Katono K, et al: Circulating tumor cells as a prognostic factor in patients with small cell lung cancer. Oncol Lett. 7:1469–1473. 2014.PubMed/NCBI
5	Romiti A, Raffa S, Di Rocco R, Roberto M, Milano A, Zullo A, Leone L, Ranieri D, Mazzetta F, Medda E, et al: Circulating tumor cells count predicts survival in colorectal cancer patients. J Gastrointestin Liver Dis. 23:279–284. 2014.PubMed/NCBI
6	Ma C, Jiang R, Li J, Wang B, Sun L and Lv Y: Research progress of lung cancer with leptomeningeal metastasis. Zhongguo Fei Ai Za Zhi. 17:695–700. 2014.(In Chinese). PubMed/NCBI
7	Wang Y, Gao Y, Zhu YF and Tao RJ: The diagnosis and treatments progress of meningeal carcinomatosis. Zhongguo Lin Chuang Shen Jing Wai Ke Za Zhi. 18:760–762. 2013.
8	Le Rhun E, Massin F, Tu Q, Bonneterre J, Mde C Bittencourt and Faure GC: Development of a new method for identification and quantification in cerebrospinal fluid of malignant cells from breast carcinoma leptomeningeal metastasis. BMC Clin Pathol. 12:212012. View Article : Google Scholar : PubMed/NCBI
9	Clarke JL, Perez HR, Jacks LM, Panageas KS and Deangelis LM: Leptomeningeal metastases in the MRI era. Neurology. 74:1449–1454. 2010. View Article : Google Scholar : PubMed/NCBI
10	Jiang R, Ma CH, Zhu ZL, Li JD, Wang B, Sun LW and Lv Y: Application of circulating tumor cell detection in cerebrospinal fluid in the diagnosis of meningeal metastasis from non-small cell lung cancer. Chin J Contemp Neurol Neurosurg. 14:698–701. 2014.
11	Ma CH, Jiang R, Li JD, Wang B, Sun LW and Lv Y: A new method for enrichment and calculation of malignant cells in cerebrospinal fluid of patients with meningeal metastasis from lung cancer. Tianjin Medical Journal. 43:419–421. 2015.
12	Wang P, She CH, Li P, Pu YZ, Wang XG and Li WL: Application of tumor markers in the adjuvant diagnosis of meningeal metastasis from lung cancer. Chin J Clin Oncol. 35:61–64. 2008.
13	Wang JH: Application of ROC curve in clinical diagnostic experiments. Chin J Hypertens. 16:175–177. 2008.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Detection value of tumor cells in cerebrospinal fluid in the diagnosis of meningeal metastasis from lung cancer by immuno-FISH technology

This article is mentioned in:

Abstract

Figure 1

Figure 2

Related Articles