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Article

miR-199a-5p regulates HIF-1α and OSGIN2 and its expression is correlated to soft-tissue sarcoma patients' outcome

  • Authors:
    • Jacqueline Keßler
    • Swetlana Rot
    • Matthias Bache
    • Matthias Kappler
    • Peter Würl
    • Dirk Vordermark
    • Helge Taubert
    • Thomas Greither
  • View Affiliations / Copyright

    Affiliations: Department of Radiotherapy, Martin Luther University Halle‑Wittenberg, D‑06120 Halle (Saale), Germany, Department of Oral and Maxillofacial Plastic Surgery, Martin Luther University Halle‑Wittenberg, D‑06120 Halle (Saale), Germany, Department of General and Visceral Surgery, Diakonie Hospital, D‑06114 Halle (Saale), Germany, Clinic of Urology, FA University Hospital Erlangen‑Nürnberg, D‑91052 Erlangen, Germany, Center for Reproductive Medicine and Andrology, Martin Luther University Halle‑Wittenberg, D‑06120 Halle (Saale), Germany
  • Pages: 5281-5288
    |
    Published online on: October 26, 2016
       https://doi.org/10.3892/ol.2016.5320
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Abstract

Soft tissue sarcomas are a heterogeneous group of malignant neoplasms of mesenchymal origin. Partly due to hypoxia, an aggressive and radioresistant phenotype frequently develops, resulting in poorer patient outcome. microRNAs (miRNAs) are tiny, non-coding regulators of gene expression and in situations of cellular stress situations may predict clinical progression and patient outcome. In the present study, hypoxia‑associated miR‑199a‑5p expression in 96 soft tissue sarcoma samples was analysed by reverse transcription‑quantitative polymerase chain reaction and associations between miR-199a-5p expression and patient clinicopathological characteristics and survival were measured. Additionally, luciferase reporter assays analyzed the post‑transcriptional regulation of hypoxia‑associated genes hypoxia‑inducible factor 1α (HIF‑1α), oxidative stress induced growth inhibitor 2 (OSGIN2) and vascular endothelial growth factor (VEGF) by miR‑199a‑5p. Survival analyses indicated that low expression of miR‑199a‑5p was significantly correlated with poorer tumor‑specific survival (univariate Cox's‑Regression analyses; relative risk=1.92, P=0.029). Furthermore, it was demonstrated that the 3'UTR of HIF‑1α and OSGIN2 genes were regulated by miR‑199a‑5p in‑vitro, although the 3'UTR of VEGF was not. To the best of our knowledge, this is the first report demonstrating the regulation of the 3'untranslated region of the OSGIN2 gene by miR-199a-5p and a significant correlation between low miR‑199a‑5p expression and a poor outcome of patients with soft tissue sarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Keßler J, Rot S, Bache M, Kappler M, Würl P, Vordermark D, Taubert H and Greither T: miR-199a-5p regulates HIF-1α and OSGIN2 and its expression is correlated to soft-tissue sarcoma patients' outcome. Oncol Lett 12: 5281-5288, 2016.
APA
Keßler, J., Rot, S., Bache, M., Kappler, M., Würl, P., Vordermark, D. ... Greither, T. (2016). miR-199a-5p regulates HIF-1α and OSGIN2 and its expression is correlated to soft-tissue sarcoma patients' outcome. Oncology Letters, 12, 5281-5288. https://doi.org/10.3892/ol.2016.5320
MLA
Keßler, J., Rot, S., Bache, M., Kappler, M., Würl, P., Vordermark, D., Taubert, H., Greither, T."miR-199a-5p regulates HIF-1α and OSGIN2 and its expression is correlated to soft-tissue sarcoma patients' outcome". Oncology Letters 12.6 (2016): 5281-5288.
Chicago
Keßler, J., Rot, S., Bache, M., Kappler, M., Würl, P., Vordermark, D., Taubert, H., Greither, T."miR-199a-5p regulates HIF-1α and OSGIN2 and its expression is correlated to soft-tissue sarcoma patients' outcome". Oncology Letters 12, no. 6 (2016): 5281-5288. https://doi.org/10.3892/ol.2016.5320
Copy and paste a formatted citation
x
Spandidos Publications style
Keßler J, Rot S, Bache M, Kappler M, Würl P, Vordermark D, Taubert H and Greither T: miR-199a-5p regulates HIF-1α and OSGIN2 and its expression is correlated to soft-tissue sarcoma patients' outcome. Oncol Lett 12: 5281-5288, 2016.
APA
Keßler, J., Rot, S., Bache, M., Kappler, M., Würl, P., Vordermark, D. ... Greither, T. (2016). miR-199a-5p regulates HIF-1α and OSGIN2 and its expression is correlated to soft-tissue sarcoma patients' outcome. Oncology Letters, 12, 5281-5288. https://doi.org/10.3892/ol.2016.5320
MLA
Keßler, J., Rot, S., Bache, M., Kappler, M., Würl, P., Vordermark, D., Taubert, H., Greither, T."miR-199a-5p regulates HIF-1α and OSGIN2 and its expression is correlated to soft-tissue sarcoma patients' outcome". Oncology Letters 12.6 (2016): 5281-5288.
Chicago
Keßler, J., Rot, S., Bache, M., Kappler, M., Würl, P., Vordermark, D., Taubert, H., Greither, T."miR-199a-5p regulates HIF-1α and OSGIN2 and its expression is correlated to soft-tissue sarcoma patients' outcome". Oncology Letters 12, no. 6 (2016): 5281-5288. https://doi.org/10.3892/ol.2016.5320
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