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Article

Jagged 2 silencing inhibits motility and invasiveness of colorectal cancer cell lines

  • Authors:
    • Wan He
    • Charles Ming Lok Chan
    • Sze Chuen Cesar Wong
    • Thomas Chi Chuen Au
    • Wing Shan Ho
    • Amanda Kit Ching Chan
    • Andrew Sai Kit Chan
    • Brigette Buig Yue Ma
    • Anthony Tak Cheung Chan
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, Shenzhen, Guangdong 518029, P.R. China, State Key Laboratory of Oncology in South China, Sir YK Pao Center for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, P.R. China, Department of Health Technology and Informatics, Hong Kong Polytechnic University, Hong Kong SAR, P.R. China, Department of Pathology, Queen Elizabeth Hospital, Hong Kong SAR, P.R. China
  • Pages: 5193-5198
    |
    Published online on: October 26, 2016
       https://doi.org/10.3892/ol.2016.5321
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Abstract

Although the Notch pathway has been reported to be activated in colorectal cancer (CRC), limited information is available regarding the expression and role of its ligand, Jagged 2 (JAG2), in CRC. Using immunohistochemistry, the present study demonstrated that JAG2 protein expression may be detected in up to 95% of CRC cases and is 3‑fold upregulated in tumor cells compared to surrounding normal tissues. This finding suggests that JAG2 may have a role in the tumorigenicity of CRC. To further investigate the cellular functions of JAG2 expression in CRC, two different small interfering RNAs (siRNAs) were used to downregulate JAG2 expression in CRC cell lines (HCT116, DLD‑1 and HT‑29). The results indicated that JAG2 knockdown inhibits the motility and invasiveness of CRC cell lines without significantly affecting cell proliferation. These findings implicate JAG2 in promoting aggressiveness of CRC, and lay the foundation for its future development as a therapeutic target for the treatment of CRC.
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Copy and paste a formatted citation
Spandidos Publications style
He W, Chan CM, Wong SC, Au TC, Ho WS, Chan AK, Chan AS, Ma BB and Chan AT: Jagged 2 silencing inhibits motility and invasiveness of colorectal cancer cell lines. Oncol Lett 12: 5193-5198, 2016.
APA
He, W., Chan, C.M., Wong, S.C., Au, T.C., Ho, W.S., Chan, A.K. ... Chan, A.T. (2016). Jagged 2 silencing inhibits motility and invasiveness of colorectal cancer cell lines. Oncology Letters, 12, 5193-5198. https://doi.org/10.3892/ol.2016.5321
MLA
He, W., Chan, C. M., Wong, S. C., Au, T. C., Ho, W. S., Chan, A. K., Chan, A. S., Ma, B. B., Chan, A. T."Jagged 2 silencing inhibits motility and invasiveness of colorectal cancer cell lines". Oncology Letters 12.6 (2016): 5193-5198.
Chicago
He, W., Chan, C. M., Wong, S. C., Au, T. C., Ho, W. S., Chan, A. K., Chan, A. S., Ma, B. B., Chan, A. T."Jagged 2 silencing inhibits motility and invasiveness of colorectal cancer cell lines". Oncology Letters 12, no. 6 (2016): 5193-5198. https://doi.org/10.3892/ol.2016.5321
Copy and paste a formatted citation
x
Spandidos Publications style
He W, Chan CM, Wong SC, Au TC, Ho WS, Chan AK, Chan AS, Ma BB and Chan AT: Jagged 2 silencing inhibits motility and invasiveness of colorectal cancer cell lines. Oncol Lett 12: 5193-5198, 2016.
APA
He, W., Chan, C.M., Wong, S.C., Au, T.C., Ho, W.S., Chan, A.K. ... Chan, A.T. (2016). Jagged 2 silencing inhibits motility and invasiveness of colorectal cancer cell lines. Oncology Letters, 12, 5193-5198. https://doi.org/10.3892/ol.2016.5321
MLA
He, W., Chan, C. M., Wong, S. C., Au, T. C., Ho, W. S., Chan, A. K., Chan, A. S., Ma, B. B., Chan, A. T."Jagged 2 silencing inhibits motility and invasiveness of colorectal cancer cell lines". Oncology Letters 12.6 (2016): 5193-5198.
Chicago
He, W., Chan, C. M., Wong, S. C., Au, T. C., Ho, W. S., Chan, A. K., Chan, A. S., Ma, B. B., Chan, A. T."Jagged 2 silencing inhibits motility and invasiveness of colorectal cancer cell lines". Oncology Letters 12, no. 6 (2016): 5193-5198. https://doi.org/10.3892/ol.2016.5321
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