RACK1 promotes hepatocellular carcinoma cell survival via CBR1 by suppressing TNF-α-induced ROS generation

  • Authors:
    • Silei Zhou
    • Huanling Cao
    • Yawei Zhao
    • Xinying Li
    • Jiyan Zhang
    • Chunmei Hou
    • Yuanfang Ma
    • Qingyang Wang
  • View Affiliations

  • Published online on: November 2, 2016     https://doi.org/10.3892/ol.2016.5339
  • Pages: 5303-5308
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Abstract

It has been reported that intracellular accumulation of reactive oxygen species (ROS) has a significant role in tumor necrosis factor (TNF)-α-induced cell apoptosis and necrosis; however, the key molecules regulating ROS generation remain to be elucidated. The present study reports that knockdown of endogenous receptor for activated C kinase 1 (RACK1) increases the intracellular ROS level following TNF‑α or H2O2 stimulation in human hepatocellular carcinoma (HCC) cells, leading to promotion of cell death. Carbonyl reductase 1 (CBR1), a ubiquitous nicotinamide adenine dinucleotide phosphate‑dependent enzyme, is reported to protect cells from ROS‑induced cell damage. The present study reports that RACK1 is a regulator of CBR1 that interacts with and sustains the protein stability of CBR1. Overexpression of CBR1 reverses the enhanced cell death due to RACK1 knockdown. Taken together, the results of the present study suggest that RACK1 protects HCC cells from TNF‑α‑induced cell death by suppressing ROS generation through interacting with and regulating CBR1.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhou S, Cao H, Zhao Y, Li X, Zhang J, Hou C, Ma Y and Wang Q: RACK1 promotes hepatocellular carcinoma cell survival via CBR1 by suppressing TNF-α-induced ROS generation. Oncol Lett 12: 5303-5308, 2016
APA
Zhou, S., Cao, H., Zhao, Y., Li, X., Zhang, J., Hou, C. ... Wang, Q. (2016). RACK1 promotes hepatocellular carcinoma cell survival via CBR1 by suppressing TNF-α-induced ROS generation. Oncology Letters, 12, 5303-5308. https://doi.org/10.3892/ol.2016.5339
MLA
Zhou, S., Cao, H., Zhao, Y., Li, X., Zhang, J., Hou, C., Ma, Y., Wang, Q."RACK1 promotes hepatocellular carcinoma cell survival via CBR1 by suppressing TNF-α-induced ROS generation". Oncology Letters 12.6 (2016): 5303-5308.
Chicago
Zhou, S., Cao, H., Zhao, Y., Li, X., Zhang, J., Hou, C., Ma, Y., Wang, Q."RACK1 promotes hepatocellular carcinoma cell survival via CBR1 by suppressing TNF-α-induced ROS generation". Oncology Letters 12, no. 6 (2016): 5303-5308. https://doi.org/10.3892/ol.2016.5339