Expression and clinical significance of Apollon in renal carcinoma

Guo,Hongbo; Zhong,Weifeng; Wang,Xiaohong; Pan,Bin; Li,Feng; Lu,Kuang; Su,Zexuan; Zhang,Shiqing

doi:10.3892/ol.2016.5349

Expression and clinical significance of Apollon in renal carcinoma

Authors:
- Hongbo Guo
- Weifeng Zhong
- Xiaohong Wang
- Bin Pan
- Feng Li
- Kuang Lu
- Zexuan Su
- Shiqing Zhang
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Affiliations: Department of Urology, No. 1 People's Hospital, Jining, Shandong 272011, P.R. China, Graduate School of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Nephrology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510630, P.R. China, Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China, Department of Urology, The Fourth Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 511447, P.R. China, Department of Urology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510630, P.R. China, Department of Urology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510630, P.R. China
Published online on: November 3, 2016 https://doi.org/10.3892/ol.2016.5349
Pages: 5129-5135
Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Apollon, namely baculoviral inhibitor of apoptosis proteins (IAP) repeat containing 6, is an unusually large member of the IAP family, and may be important in oncogenesis. The aim of the present study was to assess the association between renal carcinoma (RC) and Apollon expression, and to highlight the link between Apollon expression and the occurrence, development and prognosis of RC. Apollon expression was detected by immunohistochemistry, western blotting and reverse transcription‑quantitative polymerase chain reaction in RC tissues, adjacent non‑cancerous tissues and paired normal tissues, respectively, in order to analyze the association between Apollon expression and clinicopathological features of RC. Kaplan‑Meier survival estimate was used to assess the prognostic significance. It was observed that Apollon expression was higher in carcinoma tissues than in adjacent non‑cancerous tissues and normal control tissues at the protein and messenger RNA level (P<0.001). There was a significant difference in T‑stage (P=0.006), nodal involvement (P=0.007) and tumor‑node‑metastasis‑stage (P=0.035) in patients categorized according to different Apollon expression levels. A prognostic significance of Apollon was also identified by the Kaplan‑Meier method. The results of the present study indicate that Apollon expression is associated with the biological characteristics of renal cancer, and is potentially a valuable predictor and novel target for RC.

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