miR-574-3p acts as a tumor promoter in osteosarcoma by targeting SMAD4 signaling pathway

  • Authors:
    • Haidong Xu
    • Xiaozhou Liu
    • Juan Zhou
    • Xiaoyun Chen
    • Jianning Zhao
  • View Affiliations

  • Published online on: November 7, 2016     https://doi.org/10.3892/ol.2016.5355
  • Pages: 5247-5253
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Abstract

Human osteosarcoma is the most common primary bone malignancy sarcoma that affects primarily children and people <20 years old. In the present study, it was demonstrated that miR‑574‑3p was downregulated in human osteosarcoma U2OS, SAOS and MG63 cells lines as well as in osteosarcoma tissue compared with the normal tissues. Downregulation of miR‑574‑3p by antisense miR‑574‑3p, inhibited cell growth and induced cell apoptosis. Overexpression of miR‑574‑3p by transfection with miR‑574‑3p mimics promoted the growth of U2OS cells. The present study then identified mothers against decapentaplegic homolog 4 (SMAD4) as a target of miR‑574‑3p and SMAD4 was suppressed in miR‑574‑3p transfected cells. Overexpression of SMAD4 could rescue the promoting effects of miR‑574‑3p on cancer cell growth. In conclusion, miR‑574‑3p exerts tumor‑promoting roles by targeting the tumor‑suppressing gene SMAD4 and its downstream signaling in human osteosarcoma, which provides a novel target for the treatment.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Xu H, Liu X, Zhou J, Chen X and Zhao J: miR-574-3p acts as a tumor promoter in osteosarcoma by targeting SMAD4 signaling pathway. Oncol Lett 12: 5247-5253, 2016
APA
Xu, H., Liu, X., Zhou, J., Chen, X., & Zhao, J. (2016). miR-574-3p acts as a tumor promoter in osteosarcoma by targeting SMAD4 signaling pathway. Oncology Letters, 12, 5247-5253. https://doi.org/10.3892/ol.2016.5355
MLA
Xu, H., Liu, X., Zhou, J., Chen, X., Zhao, J."miR-574-3p acts as a tumor promoter in osteosarcoma by targeting SMAD4 signaling pathway". Oncology Letters 12.6 (2016): 5247-5253.
Chicago
Xu, H., Liu, X., Zhou, J., Chen, X., Zhao, J."miR-574-3p acts as a tumor promoter in osteosarcoma by targeting SMAD4 signaling pathway". Oncology Letters 12, no. 6 (2016): 5247-5253. https://doi.org/10.3892/ol.2016.5355