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Upregulated microRNA‑143 inhibits cell proliferation in human nasopharyngeal carcinoma

  • Authors:
    • Benfu He
    • Zhe Xu
    • Jinzhang Chen
    • Dayong Zheng
    • Aimin Li
    • Luo‑Sheng Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, PLA421 Hospital, Guangzhou, Guangdong 510318, P.R. China, Department of Pediatric Surgery, First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Oncology, Integrated Chinese and Western Medicine Hospital, Southern Medical University, Guangzhou, Guangdong 510310, P.R. China, Department of Oncology, PLA458 Hospital, Guangzhou, Guangdong 510080, P.R. China
    Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5023-5028
    |
    Published online on: November 8, 2016
       https://doi.org/10.3892/ol.2016.5363
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Abstract

The aim of the present study was to investigate the possible functions and mechanism of microRNA (miR)‑143 in cell proliferation of human nasopharyngeal carcinoma (NPC). The expression of miR‑143 in NPC cells and tissues was investigated using reverse transcription‑quantitative polymerase chain reaction. Cell viability assay, colony formation assay and flow cytometry were used to examine the cell proliferative ability and tumorigenicity. The expression levels of p21Cip1, p27Kip1, cyclin D1, phosphorylated (p)‑retinoblastoma protein (Rb), Rb and cyclin‑dependent kinase (CDK) 6 were determined by western blotting. Luciferase assay was used to confirm whether CDK6 was a direct target of miR‑143. miR‑143 was downregulated in NPC cell lines and tissues. Overexpression of miR‑143 in NPC CNE1 cells inhibited proliferation, tumorigenicity and cell cycle progression. Additionally, ectopic expression of miR‑143 downregulated cyclin D1 and p‑Rb expression, and upregulated p21Cip1 and p27Kip1 expression, which subsequently inhibited NPC cell proliferation. It was also observed that CDK6 was a direct target of miR‑143, which downregulated CDK6 expression. CDK6 suppression by miR‑143 was associated with dysregulated expression of p21Cip1, p27Kip1, cyclin D1 and p‑Rb, thereby serving an essential role in NPC cell growth. Our findings suggest that miR‑143 inhibits proliferation by targeting CDK6, and may aid to identify new targets for anti-oncomiRs.
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Copy and paste a formatted citation
Spandidos Publications style
He B, Xu Z, Chen J, Zheng D, Li A and Zhang LS: Upregulated microRNA‑143 inhibits cell proliferation in human nasopharyngeal carcinoma. Oncol Lett 12: 5023-5028, 2016.
APA
He, B., Xu, Z., Chen, J., Zheng, D., Li, A., & Zhang, L. (2016). Upregulated microRNA‑143 inhibits cell proliferation in human nasopharyngeal carcinoma. Oncology Letters, 12, 5023-5028. https://doi.org/10.3892/ol.2016.5363
MLA
He, B., Xu, Z., Chen, J., Zheng, D., Li, A., Zhang, L."Upregulated microRNA‑143 inhibits cell proliferation in human nasopharyngeal carcinoma". Oncology Letters 12.6 (2016): 5023-5028.
Chicago
He, B., Xu, Z., Chen, J., Zheng, D., Li, A., Zhang, L."Upregulated microRNA‑143 inhibits cell proliferation in human nasopharyngeal carcinoma". Oncology Letters 12, no. 6 (2016): 5023-5028. https://doi.org/10.3892/ol.2016.5363
Copy and paste a formatted citation
x
Spandidos Publications style
He B, Xu Z, Chen J, Zheng D, Li A and Zhang LS: Upregulated microRNA‑143 inhibits cell proliferation in human nasopharyngeal carcinoma. Oncol Lett 12: 5023-5028, 2016.
APA
He, B., Xu, Z., Chen, J., Zheng, D., Li, A., & Zhang, L. (2016). Upregulated microRNA‑143 inhibits cell proliferation in human nasopharyngeal carcinoma. Oncology Letters, 12, 5023-5028. https://doi.org/10.3892/ol.2016.5363
MLA
He, B., Xu, Z., Chen, J., Zheng, D., Li, A., Zhang, L."Upregulated microRNA‑143 inhibits cell proliferation in human nasopharyngeal carcinoma". Oncology Letters 12.6 (2016): 5023-5028.
Chicago
He, B., Xu, Z., Chen, J., Zheng, D., Li, A., Zhang, L."Upregulated microRNA‑143 inhibits cell proliferation in human nasopharyngeal carcinoma". Oncology Letters 12, no. 6 (2016): 5023-5028. https://doi.org/10.3892/ol.2016.5363
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