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Article

Ouabain-induced alterations in ABCB1 of mesenteric lymph nodes and thymocytes of rats and mice

  • Authors:
    • Daniel Boff Lima
    • Raphael Carmo Valente
    • Marcia Alves Marques Capella
  • View Affiliations / Copyright

    Affiliations: Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21949‑900, Brazil, Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21949‑900, Brazil
  • Pages: 5275-5280
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    Published online on: November 8, 2016
       https://doi.org/10.3892/ol.2016.5366
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Abstract

Ouabain is a glycoside with immunomodulating properties, and recent studies have suggested its use in adjuvant therapy for cancer treatment. Ouabain is known to modulate the immune system in vitro, and previous studies have revealed that ouabain can modulate the expression and activity of ABCB1, a protein associated with multidrug resistance present in immune system. Therefore, the present study investigated alterations in the expression and activity of ABCB1 in the thymi, peripheral blood monocytes and lymph nodes of Wistar rats and Swiss mice treated acutely or chronically with ouabain. A decrease of almost 45% in the monocyte count and an increase of 55% in the basophil count were observed. A significant decrease (75% reduction) in the amount of cells with ABCB1 activity was found in the thymocytes of ouabain‑treated rats and mice. The possible implications of these results for cancer treatment are discussed.
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Copy and paste a formatted citation
Spandidos Publications style
Lima DB, Valente RC and Capella MA: Ouabain-induced alterations in ABCB1 of mesenteric lymph nodes and thymocytes of rats and mice. Oncol Lett 12: 5275-5280, 2016.
APA
Lima, D.B., Valente, R.C., & Capella, M.A. (2016). Ouabain-induced alterations in ABCB1 of mesenteric lymph nodes and thymocytes of rats and mice. Oncology Letters, 12, 5275-5280. https://doi.org/10.3892/ol.2016.5366
MLA
Lima, D. B., Valente, R. C., Capella, M. A."Ouabain-induced alterations in ABCB1 of mesenteric lymph nodes and thymocytes of rats and mice". Oncology Letters 12.6 (2016): 5275-5280.
Chicago
Lima, D. B., Valente, R. C., Capella, M. A."Ouabain-induced alterations in ABCB1 of mesenteric lymph nodes and thymocytes of rats and mice". Oncology Letters 12, no. 6 (2016): 5275-5280. https://doi.org/10.3892/ol.2016.5366
Copy and paste a formatted citation
x
Spandidos Publications style
Lima DB, Valente RC and Capella MA: Ouabain-induced alterations in ABCB1 of mesenteric lymph nodes and thymocytes of rats and mice. Oncol Lett 12: 5275-5280, 2016.
APA
Lima, D.B., Valente, R.C., & Capella, M.A. (2016). Ouabain-induced alterations in ABCB1 of mesenteric lymph nodes and thymocytes of rats and mice. Oncology Letters, 12, 5275-5280. https://doi.org/10.3892/ol.2016.5366
MLA
Lima, D. B., Valente, R. C., Capella, M. A."Ouabain-induced alterations in ABCB1 of mesenteric lymph nodes and thymocytes of rats and mice". Oncology Letters 12.6 (2016): 5275-5280.
Chicago
Lima, D. B., Valente, R. C., Capella, M. A."Ouabain-induced alterations in ABCB1 of mesenteric lymph nodes and thymocytes of rats and mice". Oncology Letters 12, no. 6 (2016): 5275-5280. https://doi.org/10.3892/ol.2016.5366
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