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Article Open Access

miRNA‑129‑5p suppresses cell proliferation and invasion in lung cancer by targeting microspherule protein 1, E‑cadherin and vimentin

  • Authors:
    • Yongzhou Zhang
    • Jihong An
    • Weiling Lv
    • Tingting Lou
    • Yunxin Liu
    • Wenyi Kang
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Huaihe Hospital, Henan University, Kaifeng, Henan 475001, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5163-5169
    |
    Published online on: November 9, 2016
       https://doi.org/10.3892/ol.2016.5372
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Abstract

Downregulation of microRNA-129 (miR-129) has been described in various types of cancer, however, the significance of miR‑129 in lung cancer has not been investigated. The present study, for the first time, determined miR‑129‑5p expression levels in both lung cancer cell lines and primary lung cancer tissues and also studied the effect of miR‑129‑5p on the proliferation and invasiveness of lung cancer cells. The results showed that miR‑129‑5p expression was significantly reduced in both lung cancer cell lines and primary lung cancer tissues (P<0.05). Further research revealed that miR‑129‑5p could suppress the proliferation and invasion capability of lung cancer cells. Bioinformatics analysis suggested three cancer‑related miR‑129‑5p target genes: Microspherule protein 1 (MCRS1), E‑cadherin and vimentin. Further investigation via reverse transcription‑quantitative polymerase chain reaction and western blot analysis showed that miR‑129‑5p was able to reduce the expression levels of MCRS1 and vimentin and enhance the expression of E‑cadherin at both the messenger RNA and protein levels. The present results indicate that miR‑129‑5p is able to suppress lung cancer cell viability and invasion, which may occur via the modulating of MCRS1, E‑cadherin and vimentin expression. These findings suggest that miR‑129‑5p may be a potential biomarker and/or treatment strategy for lung cancer.
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1 

Jemal A, Siegel R, Xu J and Ward E: Cancer statistics, 2010. CA Cancer J Clin. 60:277–300. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Lagos-Quintana M, Rauhut R, Lendeckel W and Tuschl T: Identification of novel genes coding for small expressed RNAs. Science. 294:853–858. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Ambros V: MicroRNA pathways in flies and worms: Growth, death, fat, stress and timing. Cell. 113:673–676. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Calin GA, Sevignani C, Dumitru CD, Hyslop T, Noch E, Yendamuri S, Shimizu M, Rattan S, Bullrich F, Negrini M and Croce CM: Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci USA. 101:2999–3004. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Yu X, Song H, Xia T, Han S, Xiao B, Luo L, Xi Y and Guo J: Growth inhibitory effects of three miR-129 family members on gastric cancer. Gene. 532:87–93. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Bandres E, Agirre X, Bitarte N, Ramirez N, Zarate R, Roman-Gomez J, Prosper F and Garcia-Foncillas J: Epigenetic regulation of microRNA expression in colorectal cancer. Int J Cancer. 125:2737–2743. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Chen X, Hu H, Guan X, Xiong G, Wang Y, Wang K, Li J, Xu X, Yang K and Bai Y: CpG island methylation status of miRNAs in esophageal squamous cell carcinoma. Int J Cancer. 130:1607–1613. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Dyrskjot L, Ostenfeld MS, Bramsen JB, Silahtaroglu AN, Lamy P, Ramanathan R, Fristrup N, Jensen JL, Andersen CL, Zieger K, et al: Genomic profiling of microRNAs in bladder cancer: miR-129 is associated with poor outcome and promotes cell death in vitro. Cancer Res. 69:4851–4860. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, et al: Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res. 33:e1792005. View Article : Google Scholar : PubMed/NCBI

11 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Mosmann T: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 65:55–63. 1983. View Article : Google Scholar : PubMed/NCBI

13 

Shridhar R, Zhang J, Song J, Booth BA, Kevil CG, Sotiropoulou G, Sloane BF and Keppler D: Cystatin M suppresses the malignant phenotype of human MDA-MB-435S cells. Oncogene. 23:2206–2215. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Dweep H, Sticht C, Pandey P and Gretz N: miRWalk-database: Prediction of possible miRNA binding sites by ‘walking’ the genes of three genomes. J Biomed Inform. 44:839–847. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Griffiths-Jones S, Grocock RJ, van Dongen S, Bateman A and AJ: miRBase Enright: microRNA sequences, targets and gene nomenclature. Nucleic Acids Res (Database issue). 34:D140–D144. 2006. View Article : Google Scholar

16 

Ren Y, Busch RK, Perlaky L and Busch H: The 58-kDa microspherule protein (MSP58), a nucleolar protein, interacts with nucleolar protein p120. Eur J Biochem. 253:734–742. 1998. View Article : Google Scholar : PubMed/NCBI

17 

Eriksson JE, Dechat T, Grin B, Helfand B, Mendez M, Pallari HM and Goldman RD: Introducing intermediate filaments: From discovery to disease. J Clin Invest. 119:1763–1771. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Leader M, Collins M, Patel J and Henry K: Vimentin: An evaluation of its role as a tumour marker. Histopathology. 11:63–72. 1987. View Article : Google Scholar : PubMed/NCBI

19 

Gumbiner BM: Regulation of cadherin-mediated adhesion in morphogenesis. Nat Rev Mol Cell Biol. 6:622–634. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Calin GA and Croce CM: MicroRNA-cancer connection: The beginning of a new tale. Cancer Res. 66:7390–7394. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Yu X, Luo L, Wu Y, Yu X, Liu Y, Yu X, Zhao X, Zhang X, Cui L, Ye G, et al: Gastric juice miR-129 as a potential biomarker for screening gastric cancer. Med Oncol. 30:3652013. View Article : Google Scholar : PubMed/NCBI

22 

Xiao Y, Li X, Wang H, Wen R, He J and Tang J: Epigenetic regulation of miR-129-2 and its effects on the proliferation and invasion in lung cancer cells. J Cell Mol Med. 19:2172–2180. 2015.PubMed/NCBI

23 

Chiang Y, Song Y, Wang Z, Chen Y, Yue Z, Xu H, Xing C and Liu Z: Aberrant expression of miR-203 and its clinical significance in gastric and colorectal cancers. J Gastrointest Surg. 15:63–70. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Shen R, Pan S, Qi S, Lin X and Cheng S: Epigenetic repression of microRNA-129-2 leads to overexpression of SOX4 in gastric cancer. Biochem Biophys Res Commun. 394:1047–1052. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Huang YW, Liu JC, Deatherage DE, Luo J, Mutch DG, Goodfellow PJ, Miller DS and Huang TH: Epigenetic repression of microRNA-129-2 leads to overexpression of SOX4 oncogene in endometrial cancer. Cancer Res. 69:9038–9046. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H and Liu M: Down-Regulation of miR-129-5p inhibits growth and induces apoptosis in laryngeal squamous cell carcinoma by targeting APC. PLoS One. 8:e778292013. View Article : Google Scholar : PubMed/NCBI

27 

Ogawa R, Ishiguro H, Kuwabara Y, Kimura M, Mitsui A, Katada T, Harata K, Tanaka T and Fujii Y: Expression profiling of micro-RNAs in human esophageal squamous cell carcinoma using RT-PCR. Med Mol Morphol. 42:102–109. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Buscaglia LE and Li Y: Apoptosis and the target genes of microRNA-21. Chin J Cancer. 30:371–380. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Selcuklu SD, Donoghue MT and Spillane C: miR-21 as a key regulator of oncogenic processes. Biochem Soc Trans. 37:918–925. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Tili E, Michaille JJ, Gandhi V, Plunkett W, Sampath D and Calin GA: miRNAs and their potential for use against cancer and other diseases. Future Oncol. 3:521–537. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Toiyama Y, Takahashi M, Hur K, Nagasaka T, Tanaka K, Inoue Y, Kusunoki M, Boland CR and Goel A: Serum miR-21 as a diagnostic and prognostic biomarker in colorectal cancer. J Natl Cancer Inst. 105:849–859. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, Petrocca F, Visone R, Iorio M, Roldo C, Ferracin M, et al: A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci USA. 103:2257–2261. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, An J, Lv W, Lou T, Liu Y and Kang W: miRNA‑129‑5p suppresses cell proliferation and invasion in lung cancer by targeting microspherule protein 1, E‑cadherin and vimentin. Oncol Lett 12: 5163-5169, 2016.
APA
Zhang, Y., An, J., Lv, W., Lou, T., Liu, Y., & Kang, W. (2016). miRNA‑129‑5p suppresses cell proliferation and invasion in lung cancer by targeting microspherule protein 1, E‑cadherin and vimentin. Oncology Letters, 12, 5163-5169. https://doi.org/10.3892/ol.2016.5372
MLA
Zhang, Y., An, J., Lv, W., Lou, T., Liu, Y., Kang, W."miRNA‑129‑5p suppresses cell proliferation and invasion in lung cancer by targeting microspherule protein 1, E‑cadherin and vimentin". Oncology Letters 12.6 (2016): 5163-5169.
Chicago
Zhang, Y., An, J., Lv, W., Lou, T., Liu, Y., Kang, W."miRNA‑129‑5p suppresses cell proliferation and invasion in lung cancer by targeting microspherule protein 1, E‑cadherin and vimentin". Oncology Letters 12, no. 6 (2016): 5163-5169. https://doi.org/10.3892/ol.2016.5372
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, An J, Lv W, Lou T, Liu Y and Kang W: miRNA‑129‑5p suppresses cell proliferation and invasion in lung cancer by targeting microspherule protein 1, E‑cadherin and vimentin. Oncol Lett 12: 5163-5169, 2016.
APA
Zhang, Y., An, J., Lv, W., Lou, T., Liu, Y., & Kang, W. (2016). miRNA‑129‑5p suppresses cell proliferation and invasion in lung cancer by targeting microspherule protein 1, E‑cadherin and vimentin. Oncology Letters, 12, 5163-5169. https://doi.org/10.3892/ol.2016.5372
MLA
Zhang, Y., An, J., Lv, W., Lou, T., Liu, Y., Kang, W."miRNA‑129‑5p suppresses cell proliferation and invasion in lung cancer by targeting microspherule protein 1, E‑cadherin and vimentin". Oncology Letters 12.6 (2016): 5163-5169.
Chicago
Zhang, Y., An, J., Lv, W., Lou, T., Liu, Y., Kang, W."miRNA‑129‑5p suppresses cell proliferation and invasion in lung cancer by targeting microspherule protein 1, E‑cadherin and vimentin". Oncology Letters 12, no. 6 (2016): 5163-5169. https://doi.org/10.3892/ol.2016.5372
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