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Direct targeting of MAPK8IP1 by miR-10a-5p is a major mechanism for gastric cancer metastasis

  • Authors:
    • Yaoyong Lu
    • Ganbao Wei
    • Liangbo Liu
    • Yichao Mo
    • Qingsheng Chen
    • Lufei Xu
    • Rongwei Liao
    • Dehao Zeng
    • Kunqiang Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Gaozhou People's Hospital, Gaozhou, Guangdong 525200, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1131-1136
    |
    Published online on: December 28, 2016
       https://doi.org/10.3892/ol.2016.5544
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Abstract

MicroRNA (miRNA) are endogenous non‑coding RNAs that suppress gene expression at the transcriptional, post-transcriptional or translational level by targeting the 3'-UTRs of specific mRNAs. miR-10a has been frequently reported to be aberrantly overexpressed in human tumors. In gastric cancer (GC), miR‑10a has an important role in the metastasis from primary GC to lymph nodes. However, the role and relevant pathways of miR‑10a in GC metastasis remain largely unknown. The present study was performed using 41 GC and 20 normal gastric mucosa tissues. Reverse transcription-quantitative polymerase chain reaction (RT‑qPCR) analysis demonstrated that MAPK8IP1 was significant downregulated in GC tissue. A statistically significant inverse correlation was detected between miR‑10a and MAPK8IP1 mRNA expression levels in GC specimens. Luciferase reporter assay and qPCR results suggested that MAPK8IP1 was a direct target of miR‑10a in GC cells. Matrigel invasion assay and wound‑healing assay results showed that MAPK8IP1 overexpression rescued the increased migration ability of miR‑10a effectors in MKN45 cells. Furthermore, the underlying mechanism of miR‑10a functions in GC was explored. The findings indicated that miR‑10a‑5p directly targets MAPK8IP1, as a major mechanism for gastric cancer metastasis. The results of the present study suggested that miR‑10a may be a potential target for the treatment of GC in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Lu Y, Wei G, Liu L, Mo Y, Chen Q, Xu L, Liao R, Zeng D and Zhang K: Direct targeting of MAPK8IP1 by miR-10a-5p is a major mechanism for gastric cancer metastasis. Oncol Lett 13: 1131-1136, 2017.
APA
Lu, Y., Wei, G., Liu, L., Mo, Y., Chen, Q., Xu, L. ... Zhang, K. (2017). Direct targeting of MAPK8IP1 by miR-10a-5p is a major mechanism for gastric cancer metastasis. Oncology Letters, 13, 1131-1136. https://doi.org/10.3892/ol.2016.5544
MLA
Lu, Y., Wei, G., Liu, L., Mo, Y., Chen, Q., Xu, L., Liao, R., Zeng, D., Zhang, K."Direct targeting of MAPK8IP1 by miR-10a-5p is a major mechanism for gastric cancer metastasis". Oncology Letters 13.3 (2017): 1131-1136.
Chicago
Lu, Y., Wei, G., Liu, L., Mo, Y., Chen, Q., Xu, L., Liao, R., Zeng, D., Zhang, K."Direct targeting of MAPK8IP1 by miR-10a-5p is a major mechanism for gastric cancer metastasis". Oncology Letters 13, no. 3 (2017): 1131-1136. https://doi.org/10.3892/ol.2016.5544
Copy and paste a formatted citation
x
Spandidos Publications style
Lu Y, Wei G, Liu L, Mo Y, Chen Q, Xu L, Liao R, Zeng D and Zhang K: Direct targeting of MAPK8IP1 by miR-10a-5p is a major mechanism for gastric cancer metastasis. Oncol Lett 13: 1131-1136, 2017.
APA
Lu, Y., Wei, G., Liu, L., Mo, Y., Chen, Q., Xu, L. ... Zhang, K. (2017). Direct targeting of MAPK8IP1 by miR-10a-5p is a major mechanism for gastric cancer metastasis. Oncology Letters, 13, 1131-1136. https://doi.org/10.3892/ol.2016.5544
MLA
Lu, Y., Wei, G., Liu, L., Mo, Y., Chen, Q., Xu, L., Liao, R., Zeng, D., Zhang, K."Direct targeting of MAPK8IP1 by miR-10a-5p is a major mechanism for gastric cancer metastasis". Oncology Letters 13.3 (2017): 1131-1136.
Chicago
Lu, Y., Wei, G., Liu, L., Mo, Y., Chen, Q., Xu, L., Liao, R., Zeng, D., Zhang, K."Direct targeting of MAPK8IP1 by miR-10a-5p is a major mechanism for gastric cancer metastasis". Oncology Letters 13, no. 3 (2017): 1131-1136. https://doi.org/10.3892/ol.2016.5544
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