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Article

The associated pyrazolopyrimidines PP1 and PP2 inhibit protein tyrosine kinase 6 activity and suppress breast cancer cell proliferation

  • Authors:
    • Hyun Jae Shim
    • Han Ie Kim
    • Seung‑Taek Lee
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea
  • Pages: 1463-1469
    |
    Published online on: January 2, 2017
       https://doi.org/10.3892/ol.2017.5564
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Abstract

Protein tyrosine kinase (PTK)6, also known as breast tumor kinase, is a non‑receptor tyrosine kinase. It is closely associated with, but evolutionarily distinct from, the Src family members. PTK6 has a role in proliferation, migration and invasion in various cancers, and therefore has been suggested as a potentially valuable therapeutic target. In an attempt to develop PTK6 inhibitors, chemicals known to inhibit various kinases were screened for their ability to inhibit PTK6. Pyrazolopyrimidine (PP)1, PP2 and a lymphocyte‑specific protein tyrosine kinase inhibitor strongly inhibited the catalytic activity of PTK6 in vitro. These chemicals suppressed the phosphorylation of PTK6 substrate proteins, including signal transducer and activator of transcription 3, in human embryonic kidney (HEK) 293 cells expressing hyperactive PTK6. They also expressed selectivity towards PTK6 over other PTK members in HEK 293 cells. PP1 and PP2 specifically inhibited the PTK6‑dependent proliferation of human breast carcinoma T‑47D cells. PP1 and PP2 were more selective for PTK6 than for Src family kinases, and may be useful for the treatment of PTK6‑positive malignant diseases such as breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Shim HJ, Kim HI and Lee ST: The associated pyrazolopyrimidines PP1 and PP2 inhibit protein tyrosine kinase 6 activity and suppress breast cancer cell proliferation. Oncol Lett 13: 1463-1469, 2017.
APA
Shim, H.J., Kim, H.I., & Lee, S. (2017). The associated pyrazolopyrimidines PP1 and PP2 inhibit protein tyrosine kinase 6 activity and suppress breast cancer cell proliferation. Oncology Letters, 13, 1463-1469. https://doi.org/10.3892/ol.2017.5564
MLA
Shim, H. J., Kim, H. I., Lee, S."The associated pyrazolopyrimidines PP1 and PP2 inhibit protein tyrosine kinase 6 activity and suppress breast cancer cell proliferation". Oncology Letters 13.3 (2017): 1463-1469.
Chicago
Shim, H. J., Kim, H. I., Lee, S."The associated pyrazolopyrimidines PP1 and PP2 inhibit protein tyrosine kinase 6 activity and suppress breast cancer cell proliferation". Oncology Letters 13, no. 3 (2017): 1463-1469. https://doi.org/10.3892/ol.2017.5564
Copy and paste a formatted citation
x
Spandidos Publications style
Shim HJ, Kim HI and Lee ST: The associated pyrazolopyrimidines PP1 and PP2 inhibit protein tyrosine kinase 6 activity and suppress breast cancer cell proliferation. Oncol Lett 13: 1463-1469, 2017.
APA
Shim, H.J., Kim, H.I., & Lee, S. (2017). The associated pyrazolopyrimidines PP1 and PP2 inhibit protein tyrosine kinase 6 activity and suppress breast cancer cell proliferation. Oncology Letters, 13, 1463-1469. https://doi.org/10.3892/ol.2017.5564
MLA
Shim, H. J., Kim, H. I., Lee, S."The associated pyrazolopyrimidines PP1 and PP2 inhibit protein tyrosine kinase 6 activity and suppress breast cancer cell proliferation". Oncology Letters 13.3 (2017): 1463-1469.
Chicago
Shim, H. J., Kim, H. I., Lee, S."The associated pyrazolopyrimidines PP1 and PP2 inhibit protein tyrosine kinase 6 activity and suppress breast cancer cell proliferation". Oncology Letters 13, no. 3 (2017): 1463-1469. https://doi.org/10.3892/ol.2017.5564
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