FosB transcription factor regulates COX‑2 expression in colorectal cancer cells without affecting PGE2 expression

  • Authors:
    • Diana Lizeth Cervantes‑Madrid
    • Sabah Nagi
    • Annika Asting Gustafsson
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  • Published online on: January 4, 2017     https://doi.org/10.3892/ol.2017.5571
  • Pages: 1411-1416
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Abstract

The expression levels of cyclooxygenase (COX)‑2 and the prostaglandin E2 (PGE2) content have been associated with poor prognosis in patients with colorectal cancer (CRC). There is a strong correlation between COX‑2 expression and PGE2 production in tissues from CRC patients, suggesting an important role for COX‑2 on the regulation of PGE2 production. Previous studies by the present authors, where CRC patients were divided into high‑ or low‑COX‑2 expressing tumors, displayed important differences in the expression levels of several transcription factors involved in carcinogenesis. Among them, FBJ murine osteosarcoma viral oncogene homolog B (FosB), which is a member of the activator protein‑1 complex, was the highest upregulated transcription factor in patients with high expression levels of COX‑2. The present study aimed to investigate the role of FosB on the COX‑2/PGE2 axis in CRC cells with high COX‑2 expression levels. Interference RNA technology was used to knockdown FosB expression in HCA‑7 cells, and 72 h later the messenger (m)RNA expression levels of COX‑1 and COX‑2, as well as the PGE2 content, were measured. The results indicated that FosB knockdown decreased the expression levels of COX‑2 but did not affect the PGE2 content or the mRNA expression levels of COX‑1. The present findings suggest an important role for FosB on the regulation of COX‑2 expression, but no effect on the regulation of the PGE2 levels. In addition, the present results imply independent regulatory mechanisms for COX‑2 expression and PGE2 content.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Cervantes‑Madrid DL, Nagi S and Asting Gustafsson A: FosB transcription factor regulates COX‑2 expression in colorectal cancer cells without affecting PGE2 expression. Oncol Lett 13: 1411-1416, 2017
APA
Cervantes‑Madrid, D.L., Nagi, S., & Asting Gustafsson, A. (2017). FosB transcription factor regulates COX‑2 expression in colorectal cancer cells without affecting PGE2 expression. Oncology Letters, 13, 1411-1416. https://doi.org/10.3892/ol.2017.5571
MLA
Cervantes‑Madrid, D. L., Nagi, S., Asting Gustafsson, A."FosB transcription factor regulates COX‑2 expression in colorectal cancer cells without affecting PGE2 expression". Oncology Letters 13.3 (2017): 1411-1416.
Chicago
Cervantes‑Madrid, D. L., Nagi, S., Asting Gustafsson, A."FosB transcription factor regulates COX‑2 expression in colorectal cancer cells without affecting PGE2 expression". Oncology Letters 13, no. 3 (2017): 1411-1416. https://doi.org/10.3892/ol.2017.5571