miR‑455‑5p functions as a potential oncogene by targeting galectin‑9 in colon cancer

  • Authors:
    • Qianqian Yang
    • Chen Hou
    • Da Huang
    • Chunbo Zhuang
    • Weichao Jiang
    • Zhi Geng
    • Xiaobei Wang
    • Lihua Hu
  • View Affiliations

  • Published online on: January 17, 2017     https://doi.org/10.3892/ol.2017.5608
  • Pages: 1958-1964
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Abstract

Although there is evidence that galectin‑9 is a critical factor in health and disease, the upstream regulatory microRNA (miRNA or miR) of the protein remains poorly defined. miR‑455‑5p is characterized as a tumor‑associated miRNA in cancer research. However, the actual role of miR‑455‑5p with respect to inhibiting or promoting tumorigenesis in colon cancer is unclear. The present study aimed to investigate the expression, role and target regulation association of galectin‑9 and miR‑455‑5p in colon cancer. Western blot analysis and reverse transcription‑quantitative polymerase chain reaction were used for the detection of the expression levels of galectin‑9 and miRNAs. Cell Counting kit‑8 test was used for the evaluation of cell proliferation, while flow cytometry was used for cell apoptosis analysis. A potential interaction between galectin‑9 and miR‑455‑5p was predicted by target prediction programs and confirmed by luciferase assay and transfection with miRNA mimics. The present study revealed that elevated expression of galectin‑9 and miR‑455‑5p in colon cancer was associated with HT29 cell proliferation and apoptosis. Furthermore, the present study demonstrated that miR‑455‑5p reduced galectin‑9 expression by directly targeting its 3'‑untranslated region. These data suggest that miR‑455‑5p functions as a potential oncogene in colon cancer by targeting galectin‑9.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yang Q, Hou C, Huang D, Zhuang C, Jiang W, Geng Z, Wang X and Hu L: miR‑455‑5p functions as a potential oncogene by targeting galectin‑9 in colon cancer. Oncol Lett 13: 1958-1964, 2017
APA
Yang, Q., Hou, C., Huang, D., Zhuang, C., Jiang, W., Geng, Z. ... Hu, L. (2017). miR‑455‑5p functions as a potential oncogene by targeting galectin‑9 in colon cancer. Oncology Letters, 13, 1958-1964. https://doi.org/10.3892/ol.2017.5608
MLA
Yang, Q., Hou, C., Huang, D., Zhuang, C., Jiang, W., Geng, Z., Wang, X., Hu, L."miR‑455‑5p functions as a potential oncogene by targeting galectin‑9 in colon cancer". Oncology Letters 13.3 (2017): 1958-1964.
Chicago
Yang, Q., Hou, C., Huang, D., Zhuang, C., Jiang, W., Geng, Z., Wang, X., Hu, L."miR‑455‑5p functions as a potential oncogene by targeting galectin‑9 in colon cancer". Oncology Letters 13, no. 3 (2017): 1958-1964. https://doi.org/10.3892/ol.2017.5608