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Article Open Access

Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells

  • Authors:
    • Xiaolin Lin
    • Lei Tian
    • Lisha Wang
    • Wenyan Li
    • Qi Xu
    • Xiuying Xiao
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China, Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai 200032, P.R. China, Department of Oncology, Shanghai Xuhui Centre Hospital, Shanghai 200031, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1695-1701
    |
    Published online on: January 18, 2017
       https://doi.org/10.3892/ol.2017.5614
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Abstract

Licochalcone A (LCA) is a flavonoid extracted from licorice root that has antiparasitic, antibacterial and antitumor properties. Previous studies have revealed that LCA may be a novel treatment for gastric cancer. The present study further assessed the potential antitumor effects of LCA alone or in combination with 5‑fluorouracil (5‑FU), and the underlying mechanisms responsible for those effects in gastric cancer cells. The effects of LCA alone or in combination with 5‑FU on SGC7901 and MKN‑45 gastric cancer cell lines were studied using Cell Counting Kit‑8, cell cycle, apoptosis and western blot analyses of cell check points and apoptosis‑associated proteins. The results revealed that LCA inhibited cell proliferation, blocked cell cycle progression at the G2/M transition and induced apoptosis. Western blot analysis demonstrated that LCA treatment increased the levels of tumor proteins 21 and 27, as well as mouse double minute 2 homolog in gastric cancer cells. In addition, LCA treatment increased the expression levels of Bax, cleaved‑poly ADP ribose polymerase, tumor protein 53 and caspase 3, and decreased the expression levels of Bcl‑2. Therefore, the present study demonstrated that LCA alone or in combination with 5‑FU may have significant anticancer effects on gastric cancer cells, and may be a novel therapeutic for the treatment of gastric cancer in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Lin X, Tian L, Wang L, Li W, Xu Q and Xiao X: Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells. Oncol Lett 13: 1695-1701, 2017.
APA
Lin, X., Tian, L., Wang, L., Li, W., Xu, Q., & Xiao, X. (2017). Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells. Oncology Letters, 13, 1695-1701. https://doi.org/10.3892/ol.2017.5614
MLA
Lin, X., Tian, L., Wang, L., Li, W., Xu, Q., Xiao, X."Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells". Oncology Letters 13.3 (2017): 1695-1701.
Chicago
Lin, X., Tian, L., Wang, L., Li, W., Xu, Q., Xiao, X."Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells". Oncology Letters 13, no. 3 (2017): 1695-1701. https://doi.org/10.3892/ol.2017.5614
Copy and paste a formatted citation
x
Spandidos Publications style
Lin X, Tian L, Wang L, Li W, Xu Q and Xiao X: Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells. Oncol Lett 13: 1695-1701, 2017.
APA
Lin, X., Tian, L., Wang, L., Li, W., Xu, Q., & Xiao, X. (2017). Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells. Oncology Letters, 13, 1695-1701. https://doi.org/10.3892/ol.2017.5614
MLA
Lin, X., Tian, L., Wang, L., Li, W., Xu, Q., Xiao, X."Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells". Oncology Letters 13.3 (2017): 1695-1701.
Chicago
Lin, X., Tian, L., Wang, L., Li, W., Xu, Q., Xiao, X."Antitumor effects and the underlying mechanism of licochalcone A combined with 5-fluorouracil in gastric cancer cells". Oncology Letters 13, no. 3 (2017): 1695-1701. https://doi.org/10.3892/ol.2017.5614
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