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Article

Promoter methylation and expression of Raf kinase inhibitory protein in esophageal squamous cell carcinoma

  • Authors:
    • Hong Wei
    • Zhijun Liu
    • Hongyan She
    • Baoguo Liu
    • Junxia Gu
    • Dongmin Wei
    • Xiangyang Zhang
    • Jiufeng Wang
    • Shujing Qi
    • Fumin Ping
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The Affiliated Hospital of Hebei University of Engineering, Handan, Hebei 056000, P.R. China
  • Pages: 1866-1872
    |
    Published online on: January 18, 2017
       https://doi.org/10.3892/ol.2017.5617
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Abstract

Raf kinase inhibitory protein (RKIP) regulates multiple cellular processes, and its downregulation is associated with distinct human cancers. In the present study, the status of RKIP promoter methylation, as well as its expression and clinical significance in esophageal squamous cell carcinoma (ESCC), were examined. The promoter methylation status in the 5'‑CpG island of the RKIP gene and the expression level of the RKIP protein were examined using a modified methylation‑specific polymerase chain reaction (MSP) method and immunohistochemical staining, respectively, in 77 ESCC samples and matched paratumor normal tissues. The incidence of RKIP promoter methylation was significantly higher in tumor samples (75.3%) than in the matched normal tissues (27.3%; P<0.001). A higher incidence of promoter methylation was also detected in poorly differentiated cancers (93.5%) compared with well‑differentiated cancers (50.0%; P<0.001), as well as in tumor samples with positive lymph node metastasis (86.7%) compared with those with negative lymph node metastasis (59.4%; P<0.001). Consistent with the promoter methylation status, the expression level of RKIP was significantly reduced in cancer tissues (36.4%) compared with matched normal tissues (76.6%; P<0.01), as well as in cancers with positive lymph node metastasis (24.4%) compared with those with negative lymph node metastasis (53.1%; P=0.01). Promoter methylation‑induced gene silencing significantly correlated with the down regulation of RKIP and the development of ESCC. The results of the present study suggested that the methylation status of the RKIP promoter, when combined with its expression level, may serve as a biomarker for predicting the biological behaviors of ESCC.
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1 

Chen W, Zheng R, Zeng H, Zhang S and He J: Annual report on status of cancer in China, 2011. Chin J Cancer Res. 27:2–12. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Li H: Modern esophageal surgery. People's Military Medical Press; Beijing: pp. 331–332. 2004

3 

Esophageal cancer, . Epidemiology, pathogenesis and prevention. Nat Clin Pract Gastroenterol Hepatol. 5:517–526. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Holmes RS and Vaughan TL: Epidemiology and pathogenesis of esophageal cancer. Semin Radiat Oncol. 17:2–9. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Li JS, Ying JM, Wang XW, Wang ZH, Tao Q and Li LL: Promoter methylation of tumor suppressor genes in esophageal squamous cell carcinoma. Chin J Cancer. 32:3–11. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Cheng CP, Kuo IY, Alakus H, Frazer KA, Harismendy O, Wang YC and Tseng VS: Network-based analysis identifies epigenetic biomarkers of esophageal squamous cell carcinoma progression. Bioinformatics. 30:3054–3061. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Bernier I and Jollés P: Purification and characterization of a basic 23 kDa cytosolic protein from bovine brain. Biochim Biophys Acta. 790:174–181. 1984. View Article : Google Scholar : PubMed/NCBI

8 

Yeung K, Seitz T, Li S, Janosch P, McFerran B, Kaiser C, Fee F, Katsanakis KD, Rose DW, Mischak H, et al: Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP. Nature. 401:173–177. 1999. View Article : Google Scholar : PubMed/NCBI

9 

Yeung KC, Rose DW, Dhillon AS, Yaros D, Gustafsson M, Chatterjee D, McFerran B, Wyche J, Kolch W and Sedivy JM: Raf kinase inhibitor protein interacts with NF-kappaB-inducing kinase and TAK1 and inhibits NF-kappaB activation. Mol Cell Biol. 21:7207–7217. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Deiss K, Kisker C, Lohse MJ and Lorenz K: Raf kinase inhibitor protein (RKIP) dimer formation controls its target switch from Raf1 to G protein-coupled receptor kinase (GRK) 2. J Biol Chem. 287:23407–23417. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Escara-Wilke J, Yeung K and Keller ET: Raf kinase inhibitor protein (RKIP) in cancer. Cancer Metastasis Rev. 31:615–620. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Fu Z, Smith PC, Zhang L, Rubin MA, Dunn RL, Yao Z and Keller ET: Effects of raf kinase inhibitor protein expression on suppression of prostate cancer metastasis. J Natl Cancer Inst. 95:878–889. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Zhao Z, Lu P, Zhang H, Xu H, Gao N, Li M and Liu C: Nestin positively regulates the Wnt/β-catenin pathway and the proliferation, survival and invasiveness of breast cancer stem cells. Breast Cancer Res. 16:4082014. View Article : Google Scholar : PubMed/NCBI

14 

Liu C, Lu P, Lu Y, Xu H, Wang S and Chen J: Clinical implications of metastatic lymph node ratio in gastric cancer. BMC Cancer. 7:2002007. View Article : Google Scholar : PubMed/NCBI

15 

Kim HS, Won KY, Kim GY, Kim SC, Park YK and Kim YW: Reduced expression of Raf-1 kinase inhibitory protein predicts regional lymph node metastasis and shorter survival in esophageal squamous cell carcinoma. Pathol Res Pract. 208:292–299. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Gao C, Pang L, Ren C and Ma T: Prognostic value of raf kinase inhibitor protein in esophageal squamous cell carcinoma. Pathol Oncol Res. 18:471–477. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Birner P, Jesch B, Schultheis A and Schoppmann SF: RAF-kinase inhibitor protein (RKIP) downregulation in esophageal cancer and its metastases. Clin Exp Metastasis. 29:551–559. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Guo W, Dong Z, Guo Y, Lin X, Chen Z, Kuang G and Yang Z: Aberrant methylation and loss expression of RKIP is associated with tumor progression and poor prognosis in gastric cardia adenocarcinoma. Clin Exp Metastasis. 30:265–275. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Li DX, Cai HY, Wang X, Feng YL and Cai SW: Promoter methylation of Raf kinase inhibitory protein: A significant prognostic indicator for patients with gastric adenocarcinoma. Exp Ther Med. 8:844–850. 2014.PubMed/NCBI

20 

Edge SB and Compton CC: The American Joint Committee on Cancer: The 7th edition of the AJCC cancer staging manual and the future of TNM. Ann Surg Oncol. 17:1471–1474. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Rice TW, Blackstone EH and Rusch VW: 7th edition of the AJCC Cancer Staging Manual: Esophagus and esophagogastric junction. Ann Surg Oncol. 17:1721–1724. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Herman JG, Graff JR, Myöhänen S, Nelkin BD and Baylin SB: Methylation-specific PCR: A novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA. 93:9821–9826. 1996. View Article : Google Scholar : PubMed/NCBI

23 

Glickman JF, Flynn J and Reich NO: Purification and characterization of recombinant baculovirus-expressed mouse DNA methyltransferase. Biochem Biophys Res Commun. 230:280–284. 1997. View Article : Google Scholar : PubMed/NCBI

24 

Fromowitz FB, Viola MV, Chao S, Oravez S, Mishriki Y, Finkel G, Grimson R and Lundy J: ras p21 expression in the progression of breast cancer. Hum Pathol. 18:1268–1275. 1987. View Article : Google Scholar : PubMed/NCBI

25 

Baylin SB: DNA methylation and gene silencing in cancer. Nat Clin Pract Oncol. 2:(Suppl 1). S4–S11. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Kulis M and Esteller M: DNA methylation and cancer. Adv Genet. 70:27–56. 2010.PubMed/NCBI

27 

Yang CS: Vitamin nutrition and gastroesophageal cancer. J Nutr. 130:(Suppl 2S). S338–S339. 2000.

28 

Yokokawa Y, Ohta S, Hou J, Zhang XL, Li SS, Ping YM and Nakajima T: Ecological study on the risks of esophageal cancer in Ci-Xian, China: The importance of nutritional status and the use of well water. Int J Cancer. 83:620–624. 1999. View Article : Google Scholar : PubMed/NCBI

29 

Launoy G, Milan CH, Faivre J, Pienkowski P, Milan CI and Gignoux M: Alcohol, tobacco and oesophageal cancer: Effects of the duration of consumption, mean intake and current and former consumption. Br J Cancer. 75:1389–1396. 1997. View Article : Google Scholar : PubMed/NCBI

30 

Gao YB, Chen ZL, Li JG, Hu XD, Shi XJ, Sun ZM, Zhang F, Zhao ZR, Li ZT, Liu ZY, et al: Genetic landscape of esophageal squamous cell carcinoma. Nat Genet. 46:1097–1102. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Sasaki Y, Tamura M, Koyama R, Nakagaki T, Adachi Y and Tokino T: Genomic characterization of esophageal squamous cell carcinoma: Insights from next-generation sequencing. World J Gastroenterol. 22:2284–2293. 2016.PubMed/NCBI

32 

Kailasam A, Mittal SK and Agrawal DK: Epigenetics in the pathogenesis of esophageal adenocarcinoma. Clin Transl Sci. 8:394–402. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Ma K, Cao B and Guo M: The detective, prognostic, and predictive value of DNA methylation in human esophageal squamous cell carcinoma. Clin Epigenetics. 8:432016. View Article : Google Scholar : PubMed/NCBI

34 

Shames DS, Minna JD and Gazdar AF: DNA methylation in health, disease, and cancer. Curr Mol Med. 7:85–102. 2007. View Article : Google Scholar : PubMed/NCBI

35 

Jones PA, Rideout WM III, Shen JC, Spruck CH and Tsai YC: Methylation, mutation and cancer. Bioessays. 14:33–36. 1992. View Article : Google Scholar : PubMed/NCBI

36 

Pogribny I, Raiche J, Slovack M and Kovalchuk O: Dose-dependence, sex- and tissue-specificity, and persistence of radiation-induced genomic DNA methylation changes. Biochem Biophys Res Commun. 320:1253–1261. 2004. View Article : Google Scholar : PubMed/NCBI

37 

Brooks JD, Weinstein M, Lin X, Sun Y, Pin SS, Bova GS, Epstein JI, Isaacs WB and Nelson WG: CG island methylation changes near the GSTP1 gene in prostatic intraepithelial neoplasia. Cancer Epidemiol Biomarkers Prev. 7:531–536. 1998.PubMed/NCBI

38 

Chan AO and Rashid A: CpG island methylation in precursors of gastrointestinal malignancies. Curr Mol Med. 6:401–408. 2006. View Article : Google Scholar : PubMed/NCBI

39 

Li FF, Song SJ and Zhang RN: Phosphatidylethanolamine-binding protein (PEBP) in basic and clinical study. Sheng Li Ke Xue Jin Zhan. 40:214–218. 2009.(In Chinese). PubMed/NCBI

40 

Al-Mulla F, Hagan S, Al-Ali W, Jacob SP, Behbehani AI, Bitar MS, Dallol A and Kolch W: Raf kinase inhibitor protein: Mechanism of loss of expression and association with genomic instability. J Clin Pathol. 61:524–529. 2008. View Article : Google Scholar : PubMed/NCBI

41 

Eves EM, Shapiro P, Naik K, Klein UR, Trakul N and Rosner MR: Raf kinase inhibitory protein regulates aurora B kinase and the spindle checkpoint. Mol Cell. 23:561–574. 2006. View Article : Google Scholar : PubMed/NCBI

42 

Martinho O, Gouveia A, Silva P, Pimenta A, Reis RM and Lopes JM: Loss of RKIP expression is associated with poor survival in GISTs. Virchows Arch. 455:277–284. 2009. View Article : Google Scholar : PubMed/NCBI

43 

Licchesi JD and Herman JG: Methylation-specific PCR. Methods Mol Biol. 507:305–323. 2009. View Article : Google Scholar : PubMed/NCBI

44 

Bird A: Molecular biology. Methylation talk between histones and DNA. Science. 294:2113–2115. 2001. View Article : Google Scholar : PubMed/NCBI

45 

Minoo P, Zlobec I, Baker K, Tornillo L, Terracciano L, Jass JR and Lugli A: Loss of raf-1 kinase inhibitor protein expression is associated with tumor progression and metastasis in colorectal cancer. Am J Clin Pathol. 127:820–827. 2007. View Article : Google Scholar : PubMed/NCBI

46 

Hagan S, Al-Mulla F, Mallon E, Oien K, Ferrier R, Gusterson B, García JJ and Kolch W: Reduction of Raf-1 kinase inhibitor protein expression correlates with breast cancer metastasis. Clin Cancer Res. 11:7392–7397. 2005. View Article : Google Scholar : PubMed/NCBI

47 

Schuierer MM, Bataille F, Hagan S, Kolch W and Bosserhoff AK: Reduction in Raf kinase inhibitor protein expression is associated with increased Ras-extracellular signal-regulated kinase signaling in melanoma cell lines. Cancer Res. 64:5186–5192. 2004. View Article : Google Scholar : PubMed/NCBI

48 

Fu Z, Kitagawa Y, Shen R, Shah R, Mehra R, Rhodes D, Keller PJ, Mizokami A, Dunn R, Chinnaiyan AM, et al: Metastasis suppressor gene Raf kinase inhibitor protein (RKIP) is a novel prognostic marker in prostate cancer. Prostate. 66:248–256. 2006. View Article : Google Scholar : PubMed/NCBI

49 

Schuierer MM, Bataille F, Weiss TS, Hellerbrand C and Bosserhoff AK: Raf kinase inhibitor protein is downregulated in hepatocellular carcinoma. Oncol Rep. 16:451–456. 2006.PubMed/NCBI

50 

Keller ET, Fu Z, Yeung K and Brennan M: Raf kinase inhibitor protein: A prostate cancer metastasis suppressor gene. Cancer Lett. 207:131–137. 2004. View Article : Google Scholar : PubMed/NCBI

51 

Keller ET: Metastasis suppressor genes: A role for raf kinase inhibitor protein (RKIP). Anticancer Drugs. 15:663–669. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wei H, Liu Z, She H, Liu B, Gu J, Wei D, Zhang X, Wang J, Qi S, Ping F, Ping F, et al: Promoter methylation and expression of Raf kinase inhibitory protein in esophageal squamous cell carcinoma. Oncol Lett 13: 1866-1872, 2017.
APA
Wei, H., Liu, Z., She, H., Liu, B., Gu, J., Wei, D. ... Ping, F. (2017). Promoter methylation and expression of Raf kinase inhibitory protein in esophageal squamous cell carcinoma. Oncology Letters, 13, 1866-1872. https://doi.org/10.3892/ol.2017.5617
MLA
Wei, H., Liu, Z., She, H., Liu, B., Gu, J., Wei, D., Zhang, X., Wang, J., Qi, S., Ping, F."Promoter methylation and expression of Raf kinase inhibitory protein in esophageal squamous cell carcinoma". Oncology Letters 13.3 (2017): 1866-1872.
Chicago
Wei, H., Liu, Z., She, H., Liu, B., Gu, J., Wei, D., Zhang, X., Wang, J., Qi, S., Ping, F."Promoter methylation and expression of Raf kinase inhibitory protein in esophageal squamous cell carcinoma". Oncology Letters 13, no. 3 (2017): 1866-1872. https://doi.org/10.3892/ol.2017.5617
Copy and paste a formatted citation
x
Spandidos Publications style
Wei H, Liu Z, She H, Liu B, Gu J, Wei D, Zhang X, Wang J, Qi S, Ping F, Ping F, et al: Promoter methylation and expression of Raf kinase inhibitory protein in esophageal squamous cell carcinoma. Oncol Lett 13: 1866-1872, 2017.
APA
Wei, H., Liu, Z., She, H., Liu, B., Gu, J., Wei, D. ... Ping, F. (2017). Promoter methylation and expression of Raf kinase inhibitory protein in esophageal squamous cell carcinoma. Oncology Letters, 13, 1866-1872. https://doi.org/10.3892/ol.2017.5617
MLA
Wei, H., Liu, Z., She, H., Liu, B., Gu, J., Wei, D., Zhang, X., Wang, J., Qi, S., Ping, F."Promoter methylation and expression of Raf kinase inhibitory protein in esophageal squamous cell carcinoma". Oncology Letters 13.3 (2017): 1866-1872.
Chicago
Wei, H., Liu, Z., She, H., Liu, B., Gu, J., Wei, D., Zhang, X., Wang, J., Qi, S., Ping, F."Promoter methylation and expression of Raf kinase inhibitory protein in esophageal squamous cell carcinoma". Oncology Letters 13, no. 3 (2017): 1866-1872. https://doi.org/10.3892/ol.2017.5617
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