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Article Open Access

Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells

  • Authors:
    • Yan Yuan
    • Yong Du
    • Xiao‑Ye Hu
    • Mei‑Yuan Liu
    • Ji‑Ke Du
    • Xue‑Min Liu
    • Hong‑En Yu
    • Tian‑Zhu Wang
    • Jian‑Xin Pu
    • Qian Zhong
    • Qing‑Feng Zou
  • View Affiliations / Copyright

    Affiliations: Section 3 of Internal Medicine, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat‑sen University Cancer Center, Guangzhou, Guangdong 510060, P.R. China, Kunming Institute of Botany, Chinese Academy of Science, Kunming, Yunnan 650000, P.R. China
    Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1672-1680
    |
    Published online on: January 19, 2017
       https://doi.org/10.3892/ol.2017.5625
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Abstract

Cancer stem cells (CSCs) are a small proportion of tumor cells that may be responsible for tumor metastasis and recurrence. Our recent research indicated that longikaurin A (LK‑A) exhibited anti‑tumor activity in nasopharyngeal carcinoma (NPC) both in vitro and in vivo. Here, we further investigated whether LK‑A could suppress the stemness of NPC cells. Sphere formation assay was used to assess the self‑renewal ability of the cells treated with LK‑A. Side population (SP) was determined by flow cytometry to measure the influence of LK‑A on NPC SPs. The expression of the c‑myc and fibronectin was detected by western blotting. The cytotoxicity of LK‑A in combination with cisplatin to NPC cells was determined by MTT assay. Colony formation assay was used to verify whether LK‑A could sensitize NPC cells to radiation and reverse the radiotherapy resistance. In the present study, we found that LK‑A reduced the number and size of spheroid formation and decreased the SP cell percentage of the S18 cell line at a low concentration. Furthermore, LK‑A treatment downregulated the expression of c‑myc and fibronectin in NPC cell lines. Moreover, LK‑A could significantly enhance the chemotherapeutic and radiotherapeutic sensitivity of NPC cell lines and reverse acquired radiotherapy resistance of Sune2‑IR. Our data revealed that LK‑A could suppress the stemness of NPC cells and may enhance the efficacy of radiotherapy and chemotherapy.
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View References

1 

Chang ET and Adami HO: The enigmatic epidemiology of nasopharyngeal carcinoma. Cancer Epidemiol Biomarkers Prev. 15:1765–1777. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Wei KR, Zheng RS, Zhang SW, Liang ZH, Ou ZX and Chen WQ: Nasopharyngeal carcinoma incidence and mortality in China in 2010. Chin J Cancer. 33:381–387. 2014.PubMed/NCBI

3 

Xu T, Tang J, Gu M, Liu L, Wei W and Yang H: Recurrent nasopharyngeal carcinoma: A clinical dilemma and challenge. Curr Oncol. 20:e406–e419. View Article : Google Scholar : PubMed/NCBI

4 

Wang C: Radiation Therapy for Head and Neck Neoplasms. 3rd. Wiley-Liss; New York, NY: 1997

5 

Zhang Z, Filho MS and Nör JE: The biology of head and neck cancer stem cells. Oral Oncol. 48:1–9. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Clarke MF, Dick JE, Dirks PB, Eaves CJ, Jamieson CH, Jones DL, Visvader J, Weissman IL and Wahl GM: Cancer stem cells-perspectives on current status and future directions: AACR Workshop on cancer stem cells. Cancer Res. 66:9339–9344. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Ke J, Wu X, Wu X, He X, Lian L, Zou Y, He X, Wang H, Luo Y, Wang L and Lan P: A subpopulation of CD24+ cells in colon cancer cell lines possess stem cell characteristics. Neoplasma. 59:282–288. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Jaggupilli A and Elkord E: Significance of CD44 and CD24 as cancer stem cell markers: An enduring ambiguity. Clin Dev Immunol. 2012:7080362012. View Article : Google Scholar : PubMed/NCBI

9 

Ricardo S, Vieira AF, Gerhard R, Leitão D, Pinto R, Cameselle-Teijeiro JF, Milanezi F, Schmitt F and Paredes J: Breast cancer stem cell markers CD44, CD24 and ALDH1: Expression distribution within intrinsic molecular subtype. J Clin Pathol. 64:937–946. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Wang Q, Chen ZG, Du CZ, Wang HW, Yan L and Gu J: Cancer stem cell marker CD133+ tumour cells and clinical outcome in rectal cancer. Histopathology. 55:284–293. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Kondo T, Setoguchi T and Taga T: Persistence of a small subpopulation of cancer stem-like cells in the C6 glioma cell line. Proc Natl Acad Sci USA. 101:781–786. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Haraguchi N, Utsunomiya T, Inoue H, Tanaka F, Mimori K, Barnard GF and Mori M: Characterization of a side population of cancer cells from human gastrointestinal system. Stem Cells. 24:506–513. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Deng CC, Liang Y, Wu MS, Feng FT, Hu WR, Chen LZ, Feng QS, Bei JX and Zeng YX: Nigericin selectively targets cancer stem cells in nasopharyngeal carcinoma. Int J Biochem Cell Biol. 45:1997–2006. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Wu MS, Wang GF, Zhao ZQ, Liang Y, Wang HB, Wu MY, Min P, Chen LZ, Feng QS, Bei JX, et al: Smac mimetics in combination with TRAIL selectively target cancer stem cells in nasopharyngeal carcinoma. Mol Cancer Ther. 12:1728–1737. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Zou QF, Du JK, Zhang H, Wang HB, Hu ZD, Chen SP, Du Y, Li MZ, Xie D, Zou J, et al: Anti-tumour activity of longikaurin A (LK-A), a novel natural diterpenoid, in nasopharyngeal carcinoma. J Transl Med. 11:2002013. View Article : Google Scholar : PubMed/NCBI

16 

Liao YJ, Bai HY, Li ZH, Zou J, Chen JW, Zheng F, Zhang JX, Mai SJ, Zeng MS, Sun HD, et al: Longikaurin A, a natural ent-kaurane, induces G2/M phase arrest via downregulation of Skp2 and apoptosis induction through ROS/JNK/c-Jun pathway in hepatocellular carcinoma cells. Cell Death Dis. 5:e11372014. View Article : Google Scholar : PubMed/NCBI

17 

Jin ZJ: About the evaluation of drug combination. Acta Pharmacol Sin. 25:146–147. 2004.PubMed/NCBI

18 

Cao JY, Liu L, Chen SP, Zhang X, Mi YJ, Liu ZG, Li MZ, Zhang H, Qian CN, Shao JY, et al: Prognostic significance and therapeutic implications of centromere protein F expression in human nasopharyngeal carcinoma. Mol Cancer. 9:2372010. View Article : Google Scholar : PubMed/NCBI

19 

Wang J, Guo LP, Chen LZ, Zeng YX and Lu SH: Identification of cancer stem cell-like side population cells in human nasopharyngeal carcinoma cell line. Cancer Res. 67:3716–3724. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Guo Y, Zhu XD, Qu S, Li L, Su F, Li Y, Huang ST and Li DR: Identification of genes involved in radioresistance of nasopharyngeal carcinoma by integrating gene ontology and protein-protein interaction networks. Int J Oncol. 40:85–92. 2012.PubMed/NCBI

21 

Ou J, Pan F, Geng P, Wei X, Xie G, Deng J, Pang X and Liang H: Silencing fibronectin extra domain A enhances radiosensitivity in nasopharyngeal carcinomas involving an FAK/Akt/JNK pathway. Int J Radiat Oncol Biol Phys. 82:e685–e691. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Wei P, Niu M, Pan S, Zhou Y, Shuai C, Wang J, Peng S and Li G: Cancer stem-like cell: A novel target for nasopharyngeal carcinoma therapy. Stem Cell Res Ther. 5:1–9. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Nair R, Roden DL, Teo WS, McFarland A, Junankar S, Ye S, Nguyen A, Yang J, Nikolic I, Hui M, et al: c-Myc and Her2 cooperate to drive a stem-like phenotype with poor prognosis in breast cancer. Oncogene. 33:3992–4002. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Wang WJ, Wu SP, Liu JB, Shi YS, Huang X, Zhang QB and Yao KT: MYC regulation of CHK1 and CHK2 promotes radioresistance in a stem cell-like population of nasopharyngeal carcinoma cells. Cancer Res. 73:1219–1231. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Ou JJ, Wu F and Liang HJ: Colorectal tumor derived fibronectin alternatively spliced EDA domain exserts lymphangiogenic effect on human lymphatic endothelial cells. Cancer Biol Ther. 9:186–191. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Xu T, Tang J, Gu M, Liu L, Wei W and Yang H: Recurrent nasopharyngeal carcinoma: A clinical dilemma and challenge. Curr Oncol. 20:e406–e419. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Zhao S, Pu JX, Sun HD and Wu YL: Longikaurin A induces apoptosis of multiple myeloma H929 cells. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 20:611–615. 2012.(In Chinese). PubMed/NCBI

28 

French-Constant C: Alternative splicing of fibronectin-many different proteins but few different functions. Exp Cell Res. 221:261–271. 1995. View Article : Google Scholar : PubMed/NCBI

29 

Xie XY, Liu SL, Wang H, Lin L, Zhu HM, Zhang JJ and Zheng Y: The influence of fibronectin on the formation of multi-cellular spheroid of ovarian cancer. Sichuan Da Xue Xue Bao Yi Xue Ban. 45:240–244. 2014.(In Chinese). PubMed/NCBI

30 

Wang J, Huang Y, Guan Z, Zhang JL, Su HK, Zhang W, Yue CF, Yan M, Guan S and Liu QQ: E3-ligase Skp2 predicts poor prognosis and maintains cancer stem cell pool in nasopharyngeal carcinoma. Oncotarget. 5:5591–5601. 2014. View Article : Google Scholar : PubMed/NCBI

31 

O'Donnell KA, Wentzel EA, Zeller KI, Dang CV and Mendell JT: c-Myc-regulated microRNAs modulate E2F1 expression. Nature. 435:839–843. 2005. View Article : Google Scholar : PubMed/NCBI

32 

Zheng H, Ying H, Yan H, Kimmelman AC, Hiller DJ, Chen AJ, Perry SR, Tonon G, Chu GC, Ding Z, et al: Pten and p53 converge on c-Myc to control differentiation, self-renewal and transformation of normal and neoplastic stem cells in glioblastoma. Cold Spring Harb Symp Quant Biol. 73:427–437. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yuan Y, Du Y, Hu XY, Liu MY, Du JK, Liu XM, Yu HE, Wang TZ, Pu JX, Zhong Q, Zhong Q, et al: Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells. Oncol Lett 13: 1672-1680, 2017.
APA
Yuan, Y., Du, Y., Hu, X., Liu, M., Du, J., Liu, X. ... Zou, Q. (2017). Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells. Oncology Letters, 13, 1672-1680. https://doi.org/10.3892/ol.2017.5625
MLA
Yuan, Y., Du, Y., Hu, X., Liu, M., Du, J., Liu, X., Yu, H., Wang, T., Pu, J., Zhong, Q., Zou, Q."Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells". Oncology Letters 13.3 (2017): 1672-1680.
Chicago
Yuan, Y., Du, Y., Hu, X., Liu, M., Du, J., Liu, X., Yu, H., Wang, T., Pu, J., Zhong, Q., Zou, Q."Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells". Oncology Letters 13, no. 3 (2017): 1672-1680. https://doi.org/10.3892/ol.2017.5625
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan Y, Du Y, Hu XY, Liu MY, Du JK, Liu XM, Yu HE, Wang TZ, Pu JX, Zhong Q, Zhong Q, et al: Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells. Oncol Lett 13: 1672-1680, 2017.
APA
Yuan, Y., Du, Y., Hu, X., Liu, M., Du, J., Liu, X. ... Zou, Q. (2017). Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells. Oncology Letters, 13, 1672-1680. https://doi.org/10.3892/ol.2017.5625
MLA
Yuan, Y., Du, Y., Hu, X., Liu, M., Du, J., Liu, X., Yu, H., Wang, T., Pu, J., Zhong, Q., Zou, Q."Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells". Oncology Letters 13.3 (2017): 1672-1680.
Chicago
Yuan, Y., Du, Y., Hu, X., Liu, M., Du, J., Liu, X., Yu, H., Wang, T., Pu, J., Zhong, Q., Zou, Q."Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells". Oncology Letters 13, no. 3 (2017): 1672-1680. https://doi.org/10.3892/ol.2017.5625
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