Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study

Pinheiro,Carla  Aparecida; Soares,Iberê  Cauduro; Penna,Valter; Squire,Jeremy; Reis,Rui   Manuel Vieira; da Silva,Sandra   Regina Morini; de Carvalho,Isabela; Camparoto,Marjori  Leiva; da Silva,Maicon   Fernando Zanon; Longatto Filho,Adhemar

doi:10.3892/ol.2017.5633

March-2017 Volume 13 Issue 3

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March-2017 Volume 13 Issue 3

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Article

Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study

Authors:
- Carla Aparecida Pinheiro
- Iberê Cauduro Soares
- Valter Penna
- Jeremy Squire
- Rui Manuel Vieira Reis
- Sandra Regina Morini da Silva
- Isabela de Carvalho
- Marjori Leiva Camparoto
- Maicon Fernando Zanon da Silva
- Adhemar Longatto Filho
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Affiliations: Barretos Cancer Hospital, Pio XII Foundation, Barretos, São Paulo 14780‑000, Portugal, Paulista State University, Botucatu, São Paulo 18607‑741, Portugal, Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo 14049‑900, Portugal, Molecular Oncology Research Center, Barretos Cancer Hospital, Pio XII Foundation, Barretos, São Paulo 14780‑000, Portugal
Pages: 1835-1835
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Published online on: January 23, 2017

https://doi.org/10.3892/ol.2017.5633
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Abstract

The genetics background underlying the aggressiveness of chondrosarcoma (CS) is poorly understood. One possible cause of malignant transformation is chromosomal instability, which involves an error in mitotic segregation due to numerical and/or functional abnormalities of centrosomes. The present study aimed to evaluate centrosome amplification in cryopreserved samples of tumor tissue from patients with CS. An analysis was performed on 3 primary cultures of tumors from patients who underwent surgery between January 2012 and December 2012 at the Department of Orthopedics at the Barretos Cancer Hospital (Barretos, Brazil). Additionally, cryopreserved tumor specimens were analyzed from 10 patients. The data were assessed using immunocytochemistry and immunohistochemistry staining techniques with monoclonal antibody anti‑γ‑tubulin. A total of 4 samples of CS cultured cells were obtained from 3 patients. A recurrence of a histological grade III tumor was detected in a female patient with Ollier's syndrome. The other 2 cases were grade I and III. The incidence of centrosome amplification in the primary cultures ranged from 15‑64% of the cells. Whereas control cultured fibroblasts showed baseline levels of 4% amplified cells. For the cryopreserved specimens, two independent observers analyzed each sample and counted the cells stained with γ‑tubulin, verifying the percentage of affected cells to be a mean of 14%, with the number of clusters ranging between 0‑6 per slide. In conclusion, centrosome amplification was found to be a consistent biological feature of CS and may underlie chromosomal instability in this tumor.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study

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