Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study

  • Authors:
    • Carla Aparecida Pinheiro
    • Iberê Cauduro Soares
    • Valter Penna
    • Jeremy Squire
    • Rui Manuel Vieira Reis
    • Sandra Regina Morini da Silva
    • Isabela de Carvalho
    • Marjori Leiva Camparoto
    • Maicon Fernando Zanon da Silva
    • Adhemar Longatto Filho
  • View Affiliations

  • Published online on: January 23, 2017     https://doi.org/10.3892/ol.2017.5633
  • Pages: 1835-1835
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Abstract

The genetics background underlying the aggressiveness of chondrosarcoma (CS) is poorly understood. One possible cause of malignant transformation is chromosomal instability, which involves an error in mitotic segregation due to numerical and/or functional abnormalities of centrosomes. The present study aimed to evaluate centrosome amplification in cryopreserved samples of tumor tissue from patients with CS. An analysis was performed on 3 primary cultures of tumors from patients who underwent surgery between January 2012 and December 2012 at the Department of Orthopedics at the Barretos Cancer Hospital (Barretos, Brazil). Additionally, cryopreserved tumor specimens were analyzed from 10 patients. The data were assessed using immunocytochemistry and immunohistochemistry staining techniques with monoclonal antibody anti‑γ‑tubulin. A total of 4 samples of CS cultured cells were obtained from 3 patients. A recurrence of a histological grade III tumor was detected in a female patient with Ollier's syndrome. The other 2 cases were grade I and III. The incidence of centrosome amplification in the primary cultures ranged from 15‑64% of the cells. Whereas control cultured fibroblasts showed baseline levels of 4% amplified cells. For the cryopreserved specimens, two independent observers analyzed each sample and counted the cells stained with γ‑tubulin, verifying the percentage of affected cells to be a mean of 14%, with the number of clusters ranging between 0‑6 per slide. In conclusion, centrosome amplification was found to be a consistent biological feature of CS and may underlie chromosomal instability in this tumor.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Pinheiro CA, Soares IC, Penna V, Squire J, Reis RM, da Silva SR, de Carvalho I, Camparoto ML, da Silva MF, Longatto Filho A , Longatto Filho A , et al: Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study. Oncol Lett 13: 1835-1835, 2017.
APA
Pinheiro, C.A., Soares, I.C., Penna, V., Squire, J., Reis, R.M., da Silva, S.R. ... Longatto Filho, A. . (2017). Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study. Oncology Letters, 13, 1835-1835. https://doi.org/10.3892/ol.2017.5633
MLA
Pinheiro, C. A., Soares, I. C., Penna, V., Squire, J., Reis, R. M., da Silva, S. R., de Carvalho, I., Camparoto, M. L., da Silva, M. F., Longatto Filho, A. ."Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study". Oncology Letters 13.3 (2017): 1835-1835.
Chicago
Pinheiro, C. A., Soares, I. C., Penna, V., Squire, J., Reis, R. M., da Silva, S. R., de Carvalho, I., Camparoto, M. L., da Silva, M. F., Longatto Filho, A. ."Centrosome amplification in chondrosarcomas: A primary cell culture and cryopreserved tumor sample study". Oncology Letters 13, no. 3 (2017): 1835-1835. https://doi.org/10.3892/ol.2017.5633