Open Access

Smad3 mutant mice develop colon cancer with overexpression of COX‑2

  • Authors:
    • Yu‑Ping Zhu
    • Zhuo Liu
    • Zhi‑Xuan Fu
    • De‑Chuan Li
  • View Affiliations

  • Published online on: January 25, 2017     https://doi.org/10.3892/ol.2017.5639
  • Pages: 1535-1538
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Colon cancer is the second most common cause of cancer‑associated mortality in human populations. The aim of the present study was to identify the role of cyclooxygenase‑2 (COX‑2) in Smad3 mutant mice, which are known to develop colon cancer. Homozygous Smad3 (‑/‑) mutant mice were generated from inbred and hybrid Smad3 mouse strains by intercrossing the appropriate heterozygotes. Immunohistochemistry with COX‑2 antibody was performed throughout this experiment and the data was validated and cross‑checked with reverse transcription‑polymerase chain reaction (RT‑PCR). Homozygous mutant Smad3 mice were generated and the overexpression pattern of COX‑2 was identified by immunohistochemistry and validated with RT‑PCR. The results of the present study demonstrated a link between the Smad3 mutant mice, colon cancer and COX‑2. In addition, the overexpression pattern of COX‑2 in Smad3 mutant mice that develop colon cancer was identified.
View Figures
View References

Related Articles

Journal Cover

March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhu YP, Liu Z, Fu ZX and Li DC: Smad3 mutant mice develop colon cancer with overexpression of COX‑2. Oncol Lett 13: 1535-1538, 2017
APA
Zhu, Y., Liu, Z., Fu, Z., & Li, D. (2017). Smad3 mutant mice develop colon cancer with overexpression of COX‑2. Oncology Letters, 13, 1535-1538. https://doi.org/10.3892/ol.2017.5639
MLA
Zhu, Y., Liu, Z., Fu, Z., Li, D."Smad3 mutant mice develop colon cancer with overexpression of COX‑2". Oncology Letters 13.3 (2017): 1535-1538.
Chicago
Zhu, Y., Liu, Z., Fu, Z., Li, D."Smad3 mutant mice develop colon cancer with overexpression of COX‑2". Oncology Letters 13, no. 3 (2017): 1535-1538. https://doi.org/10.3892/ol.2017.5639