Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
March-2017 Volume 13 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2017 Volume 13 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Smad3 mutant mice develop colon cancer with overexpression of COX‑2

  • Authors:
    • Yu‑Ping Zhu
    • Zhuo Liu
    • Zhi‑Xuan Fu
    • De‑Chuan Li
  • View Affiliations / Copyright

    Affiliations: Department of Colorectal Cancer Surgery, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1535-1538
    |
    Published online on: January 25, 2017
       https://doi.org/10.3892/ol.2017.5639
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Colon cancer is the second most common cause of cancer‑associated mortality in human populations. The aim of the present study was to identify the role of cyclooxygenase‑2 (COX‑2) in Smad3 mutant mice, which are known to develop colon cancer. Homozygous Smad3 (‑/‑) mutant mice were generated from inbred and hybrid Smad3 mouse strains by intercrossing the appropriate heterozygotes. Immunohistochemistry with COX‑2 antibody was performed throughout this experiment and the data was validated and cross‑checked with reverse transcription‑polymerase chain reaction (RT‑PCR). Homozygous mutant Smad3 mice were generated and the overexpression pattern of COX‑2 was identified by immunohistochemistry and validated with RT‑PCR. The results of the present study demonstrated a link between the Smad3 mutant mice, colon cancer and COX‑2. In addition, the overexpression pattern of COX‑2 in Smad3 mutant mice that develop colon cancer was identified.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Potter JD, Slattery ML, Bostick RM and Gapstur SM: Colon cancer: a review of the epidemiology. Epidemiol Rev. 15:499–545. 1993. View Article : Google Scholar : PubMed/NCBI

2 

Siegel R, Desantis C and Jemal A: Colorectal cancer statistics, 2014. CA Cancer J Clin. 64:104–117. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Tomlinson I, Ilyas M and Novelli M: Molecular genetics of colon cancer. Cancer Metastasis Rev. 16:67–79. 1997. View Article : Google Scholar : PubMed/NCBI

4 

Zhu Y, Richardson JA, Parada LF and Graff JM: Smad3 mutant mice develop metastatic colorectal cancer. Cell. 94:703–714. 1998. View Article : Google Scholar : PubMed/NCBI

5 

Oshima M, Oshima H and Taketo MM: TGF-beta receptor type II deficiency results in defects of yolk sac hematopoiesis and vasculogenesis. Dev Biol. 179:297–302. 1996. View Article : Google Scholar : PubMed/NCBI

6 

Liu X, Sun Y, Constantinescu SN, Karam E, Weinberg RA and Lodish HF: Transforming growth factor beta-induced phosphorylation of Smad3 is required for growth inhibition and transcriptional induction in epithelial cells. Proc Natl Acad Sci USA. 94:10669–10674. 1997. View Article : Google Scholar : PubMed/NCBI

7 

Graff JM, Bansal A and Melton DA: Xenopus Mad proteins transduce distinct subsets of signals for the TGF beta superfamily. Cell. 85:479–487. 1996. View Article : Google Scholar : PubMed/NCBI

8 

Brown JR and DuBois RN: COX-2: A molecular target for colorectal cancer prevention. J Clin Oncol. 23:2840–2855. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Soumaoro LT, Uetake H, Higuchi T, Takagi Y, Enomoto M and Sugihara K: Cyclooxygenase-2 expression: A significant prognostic indicator for patients with colorectal cancer. Clin Cancer Res. 10:8465–8471. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Buchanan FG and DuBois RN: Connecting COX-2 and Wnt in cancer. Cancer Cell. 9:6–8. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Humphreys BD, Valerius MT, Kobayashi A, Mugford JW, Soeung S, Duffield JS, McMahon AP and Bonventre JV: Intrinsic epithelial cells repair the kidney after injury. Cell Stem Cell. 2:284–291. 2008. View Article : Google Scholar : PubMed/NCBI

12 

National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals, . Guide for the Care and Use of Laboratory Animals. 8th. National Academies Press (US); Washington DC: 2011, PubMed/NCBI

13 

Shea K and Geijsen N: Dissection of 6.5 dpc mouse embryos. J Vis Exp. 2:e16010.3791/160. 2007.

14 

Hogan B, Costantini F and Lacy E: Manipulating the Mouse Embryo: A Laboratory Manual. 1st. Cold Spring Harbor Laboratory Press; NY: pp. 55–60. 1986

15 

Zhong X, Wang H and Jian X: Expression of matrix metalloproteinases-8 and −9 and their tissue inhibitor in the condyles of diabetic rats with mandibular advancement. Exp Ther Med. 8:1357–1364. 2014.PubMed/NCBI

16 

Ogino S, Meyerhardt JA, Cantor M, Brahmandam M, Clark JW, Namgyal C, Kawasaki T, Kinsella K, Michelini AL, Enzinger PC, et al: Molecular alterations in tumors and response to combination chemotherapy with gefitinib for advanced colorectal cancer. Clin Cancer Res. 11:6650–6656. 2005. View Article : Google Scholar : PubMed/NCBI

17 

de Heer P, Gosens MJ, de Bruin EC, Dekker-Ensink NG, Putter H, Marijnen CA, van den Brule AJ, van Krieken JH, Rutten HJ, Kuppen PJ and van de Velde CJ: Dutch Colorectal Cancer Group: Cyclooxygenase 2 expression in rectal cancer is of prognostic significance in patients receiving preoperative radiotherapy. Clin Cancer Res. 13:2955–2960. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Zlobec I, Terracciano LM and Lugli A: Local recurrence in mismatch repair-proficient colon cancer predicted by an infiltrative tumor border and lack of CD8+ tumor-infiltrating lymphocytes. Clin Cancer Res. 14:3792–3797. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Henry LR, Lee HO, Lee JS, Klein-Szanto A, Watts P, Ross EA, Chen WT and Cheng JD: Clinical implications of fibroblast activation protein in patients with colon cancer. Clin Cancer Res. 13:1736–1741. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Ginty F, Adak S, Can A, Gerdes M, Larsen M, Cline H, Filkins R, Pang Z, Li Q and Montalto MC: The relative distribution of membranous and cytoplasmic met is a prognostic indicator in stage I and II colon cancer. Clin Cancer Res. 14:3814–3822. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Benson AB III: New approaches to assessing and treating early-stage colon and rectal cancers: Cooperative group strategies for assessing optimal approaches in early-stage disease. Clin Cancer Res. 13:6913s–6920s. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Morris M, Platell C and Iacopetta B: Tumor-infiltrating lymphocytes and perforation in colon cancer predict positive response to 5-fluorouracil chemotherapy. Clin Cancer Res. 14:1413–1417. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Zhu Y, Richardson JA, Parada LF and Graff JM: Smad3 mutant mice develop metastatic colorectal cancer. Cell. 94:703–714. 1998. View Article : Google Scholar : PubMed/NCBI

24 

He ZY, Shi CB, Wen H, Li FL, Wang BL and Wang J: Upregulation of p53 expression in patients with colorectal cancer by administration of curcumin. Cancer Invest. 29:208–213. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhu YP, Liu Z, Fu ZX and Li DC: Smad3 mutant mice develop colon cancer with overexpression of COX‑2. Oncol Lett 13: 1535-1538, 2017.
APA
Zhu, Y., Liu, Z., Fu, Z., & Li, D. (2017). Smad3 mutant mice develop colon cancer with overexpression of COX‑2. Oncology Letters, 13, 1535-1538. https://doi.org/10.3892/ol.2017.5639
MLA
Zhu, Y., Liu, Z., Fu, Z., Li, D."Smad3 mutant mice develop colon cancer with overexpression of COX‑2". Oncology Letters 13.3 (2017): 1535-1538.
Chicago
Zhu, Y., Liu, Z., Fu, Z., Li, D."Smad3 mutant mice develop colon cancer with overexpression of COX‑2". Oncology Letters 13, no. 3 (2017): 1535-1538. https://doi.org/10.3892/ol.2017.5639
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu YP, Liu Z, Fu ZX and Li DC: Smad3 mutant mice develop colon cancer with overexpression of COX‑2. Oncol Lett 13: 1535-1538, 2017.
APA
Zhu, Y., Liu, Z., Fu, Z., & Li, D. (2017). Smad3 mutant mice develop colon cancer with overexpression of COX‑2. Oncology Letters, 13, 1535-1538. https://doi.org/10.3892/ol.2017.5639
MLA
Zhu, Y., Liu, Z., Fu, Z., Li, D."Smad3 mutant mice develop colon cancer with overexpression of COX‑2". Oncology Letters 13.3 (2017): 1535-1538.
Chicago
Zhu, Y., Liu, Z., Fu, Z., Li, D."Smad3 mutant mice develop colon cancer with overexpression of COX‑2". Oncology Letters 13, no. 3 (2017): 1535-1538. https://doi.org/10.3892/ol.2017.5639
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team