MicroRNA-134 targets KRAS to suppress breast cancer cell proliferation, migration and invasion

  • Authors:
    • Xiaomei Su
    • Ling Zhang
    • Hua Li
    • Peng Cheng
    • Yajie Zhu
    • Zhen Liu
    • Yu Zhao
    • Hongyu Xu
    • Dong Li
    • Hui Gao
    • Tao Zhang
  • View Affiliations

  • Published online on: January 25, 2017     https://doi.org/10.3892/ol.2017.5644
  • Pages: 1932-1938
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Abstract

The expression patterns and functions of microRNA-134 (miR-134) have been previously studied in numerous types of cancer. To the best of our knowledge, this is the first study of miR-134 in human breast cancer. In the present study, the expression patterns, biological functions and underlying molecular mechanisms of miR‑134 in human breast cancer were investigated. Reverse transcription‑quantitative polymerase chain reaction evaluated the expression of miR‑134 in human breast cancer tissues, matched normal adjacent tissues, breast cancer cell lines and a normal mammary epithelial cell line. Following transfection with miR‑134, an MTT assay, cell migration assay, cell invasion assay, western blot analysis and a luciferase assay were performed on the MCF‑7 and MDA‑MB‑231 human breast cancer cell lines. The findings revealed that miR‑134 expression levels were significantly downregulated in breast cancer cells. Statistical analysis demonstrated that low expression of miR‑134 was significantly associated with lymph node metastasis, TNM stage and reduced cell differentiation. It was observed that miR‑134 inhibited the growth, migration and invasion of breast cancer cells. Additionally, the present study indicated that miR‑134 may directly target the Kirsten rat sarcoma viral oncogene homolog in breast cancer tissues. These results suggest that miR‑134 may be used as a potential therapeutic biomarker in breast cancers.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Su X, Zhang L, Li H, Cheng P, Zhu Y, Liu Z, Zhao Y, Xu H, Li D, Gao H, Gao H, et al: MicroRNA-134 targets KRAS to suppress breast cancer cell proliferation, migration and invasion. Oncol Lett 13: 1932-1938, 2017
APA
Su, X., Zhang, L., Li, H., Cheng, P., Zhu, Y., Liu, Z. ... Zhang, T. (2017). MicroRNA-134 targets KRAS to suppress breast cancer cell proliferation, migration and invasion. Oncology Letters, 13, 1932-1938. https://doi.org/10.3892/ol.2017.5644
MLA
Su, X., Zhang, L., Li, H., Cheng, P., Zhu, Y., Liu, Z., Zhao, Y., Xu, H., Li, D., Gao, H., Zhang, T."MicroRNA-134 targets KRAS to suppress breast cancer cell proliferation, migration and invasion". Oncology Letters 13.3 (2017): 1932-1938.
Chicago
Su, X., Zhang, L., Li, H., Cheng, P., Zhu, Y., Liu, Z., Zhao, Y., Xu, H., Li, D., Gao, H., Zhang, T."MicroRNA-134 targets KRAS to suppress breast cancer cell proliferation, migration and invasion". Oncology Letters 13, no. 3 (2017): 1932-1938. https://doi.org/10.3892/ol.2017.5644