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Article Open Access

Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors

  • Authors:
    • Ann Kakabadze
    • Konstantine Mardaleishvili
    • George Loladze
    • Lia Karalashvili
    • Gocha Chutkerashvili
    • David Chakhunashvili
    • Zurab Kakabadze
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Anatomy, Tbilisi State Medical University, 0177 Tbilisi, Georgia, Department of Surgery, Cancer Research Center, 0177 Tbilisi, Georgia, Department of Molecular and Translational Medicine, Institute of Medical Research, Ilia State University, 0177 Tbilisi, Georgia
    Copyright: © Kakabadze et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1811-1818
    |
    Published online on: January 25, 2017
       https://doi.org/10.3892/ol.2017.5647
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Abstract

The gold standard following segmental mandibulectomy is vascularized autologous bone graft in the form of the fibula flap. However, in bone reconstruction the use of autogenous bone does not always guarantee a successful outcome. The aim of the present investigation was to develop a novel biologically active bone (BAB) graft, and to use it for the reconstruction of large size defects of the mandible bone following tumor resection. In the first part of the present study, biologically active bone graft was developed by using human freeze‑dried bone marrow stem cells (BMSCs) paracrine factors and three‑dimensional bone scaffold derived from cancellous bovine bone following decellularization. In the second part of the research, one male and three female patients with primary tumors of the mandible underwent hemimandibulectomy. The mandibular bone defects following tumor resection were reconstructed with autogenous rib grafts in three patients and BAB graft was used in one patient. The graft‑host interfaces were covered with decellularized human amnion/chorion membrane graft. All patients were followed‑up every five months following the reconstruction of the mandible, with no complications observed. Preliminary clinical investigations demonstrated that a BAB graft containing freeze‑dried BMSC paracrine factors may be used for the reconstruction of large mandibular bone defects following tumor resection.
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Copy and paste a formatted citation
Spandidos Publications style
Kakabadze A, Mardaleishvili K, Loladze G, Karalashvili L, Chutkerashvili G, Chakhunashvili D and Kakabadze Z: Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors. Oncol Lett 13: 1811-1818, 2017.
APA
Kakabadze, A., Mardaleishvili, K., Loladze, G., Karalashvili, L., Chutkerashvili, G., Chakhunashvili, D., & Kakabadze, Z. (2017). Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors. Oncology Letters, 13, 1811-1818. https://doi.org/10.3892/ol.2017.5647
MLA
Kakabadze, A., Mardaleishvili, K., Loladze, G., Karalashvili, L., Chutkerashvili, G., Chakhunashvili, D., Kakabadze, Z."Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors". Oncology Letters 13.3 (2017): 1811-1818.
Chicago
Kakabadze, A., Mardaleishvili, K., Loladze, G., Karalashvili, L., Chutkerashvili, G., Chakhunashvili, D., Kakabadze, Z."Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors". Oncology Letters 13, no. 3 (2017): 1811-1818. https://doi.org/10.3892/ol.2017.5647
Copy and paste a formatted citation
x
Spandidos Publications style
Kakabadze A, Mardaleishvili K, Loladze G, Karalashvili L, Chutkerashvili G, Chakhunashvili D and Kakabadze Z: Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors. Oncol Lett 13: 1811-1818, 2017.
APA
Kakabadze, A., Mardaleishvili, K., Loladze, G., Karalashvili, L., Chutkerashvili, G., Chakhunashvili, D., & Kakabadze, Z. (2017). Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors. Oncology Letters, 13, 1811-1818. https://doi.org/10.3892/ol.2017.5647
MLA
Kakabadze, A., Mardaleishvili, K., Loladze, G., Karalashvili, L., Chutkerashvili, G., Chakhunashvili, D., Kakabadze, Z."Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors". Oncology Letters 13.3 (2017): 1811-1818.
Chicago
Kakabadze, A., Mardaleishvili, K., Loladze, G., Karalashvili, L., Chutkerashvili, G., Chakhunashvili, D., Kakabadze, Z."Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors". Oncology Letters 13, no. 3 (2017): 1811-1818. https://doi.org/10.3892/ol.2017.5647
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