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Article

Characterization of topoisomerase II α and minichromosome maintenance protein 2 expression in anal carcinoma

  • Authors:
    • Cristovam Scapulatempo‑Neto
    • Carlos Veo
    • José Humberto T.G. Fregnani
    • Adriana Lorenzi
    • Allini Mafra
    • Armando G.F. Melani
    • Edgar Antonio Alemán Loaiza
    • Luciana Albina Reis Rosa
    • Cristina Mendes de Oliveira
    • José Eduardo Levi
    • Adhemar Longatto‑Filho
  • View Affiliations / Copyright

    Affiliations: Department of Pathology (CSN), Barretos Cancer Hospital, Pio XII Foundation, Barretos, São Paulo, SP 14784‑400, Brazil, Department of Surgery, Barretos Cancer Hospital, Pio XII Foundation, Barretos, São Paulo, SP 14784‑400, Brazil, Molecular Oncology Research Center, Barretos Cancer Hospital, Pio XII Foundation, Barretos, São Paulo, SP 14784‑400, Brazil, Laboratory of Virology, Institute of Tropical Medicine São Paulo, University of São Paulo, São Paulo, SP 05403‑000, Brazil
  • Pages: 1891-1898
    |
    Published online on: January 25, 2017
       https://doi.org/10.3892/ol.2017.5650
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Abstract

The present study aimed to ascertain the significance of topoisomerase II α (TOP2A) and minichromosome maintenance protein (MCM) 2 expression in anal carcinoma. A total of 75 anal lesions were retrieved from the files of the Department of Pathology of Barretos Cancer Hospital (Barretos, Brazil) in order to verify the human papillomavirus (HPV) statuses of these lesions and characterize the immunohistochemical expression levels of TOP2A and MCM2 in anal carcinoma, as these are important markers for cervical HPV‑induced lesions; their expression was also compared with respect to p16 and Ki‑67. The vast majority of the cases tested positive for HPV16 (84%); 1 case tested positive for both HPV16 and HPV18. Positive HPV16 status was more frequent in early stages than in advanced stages (P=0.008). Positive immunohistochemical reactivity for MCM2 and TOP2A protein was observed in 71.6 and 100% of cases, respectively. Positive reactivity for p16 was significantly associated (P=0.001) with histological grade, and was more commonly expressed in squamous cell carcinoma than adenocarcinomas. HPV16 was strongly associated with positive p16 protein expression (76.6%). However, the high expression of Ki‑67 combined with the high expression of p16 was predominantly observed in Stage III‑IV cases. MCM2, TOP2A, p16 and Ki‑67 exhibited intense positive staining in the anal lesions, indicating that these markers were significantly and constantly expressed in anal carcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Scapulatempo‑Neto C, Veo C, Fregnani JH, Lorenzi A, Mafra A, Melani AG, Loaiza EA, Rosa LA, de Oliveira CM, Levi JE, Levi JE, et al: Characterization of topoisomerase II α and minichromosome maintenance protein 2 expression in anal carcinoma. Oncol Lett 13: 1891-1898, 2017.
APA
Scapulatempo‑Neto, C., Veo, C., Fregnani, J.H., Lorenzi, A., Mafra, A., Melani, A.G. ... Longatto‑Filho, A. (2017). Characterization of topoisomerase II α and minichromosome maintenance protein 2 expression in anal carcinoma. Oncology Letters, 13, 1891-1898. https://doi.org/10.3892/ol.2017.5650
MLA
Scapulatempo‑Neto, C., Veo, C., Fregnani, J. H., Lorenzi, A., Mafra, A., Melani, A. G., Loaiza, E. A., Rosa, L. A., de Oliveira, C. M., Levi, J. E., Longatto‑Filho, A."Characterization of topoisomerase II α and minichromosome maintenance protein 2 expression in anal carcinoma". Oncology Letters 13.3 (2017): 1891-1898.
Chicago
Scapulatempo‑Neto, C., Veo, C., Fregnani, J. H., Lorenzi, A., Mafra, A., Melani, A. G., Loaiza, E. A., Rosa, L. A., de Oliveira, C. M., Levi, J. E., Longatto‑Filho, A."Characterization of topoisomerase II α and minichromosome maintenance protein 2 expression in anal carcinoma". Oncology Letters 13, no. 3 (2017): 1891-1898. https://doi.org/10.3892/ol.2017.5650
Copy and paste a formatted citation
x
Spandidos Publications style
Scapulatempo‑Neto C, Veo C, Fregnani JH, Lorenzi A, Mafra A, Melani AG, Loaiza EA, Rosa LA, de Oliveira CM, Levi JE, Levi JE, et al: Characterization of topoisomerase II α and minichromosome maintenance protein 2 expression in anal carcinoma. Oncol Lett 13: 1891-1898, 2017.
APA
Scapulatempo‑Neto, C., Veo, C., Fregnani, J.H., Lorenzi, A., Mafra, A., Melani, A.G. ... Longatto‑Filho, A. (2017). Characterization of topoisomerase II α and minichromosome maintenance protein 2 expression in anal carcinoma. Oncology Letters, 13, 1891-1898. https://doi.org/10.3892/ol.2017.5650
MLA
Scapulatempo‑Neto, C., Veo, C., Fregnani, J. H., Lorenzi, A., Mafra, A., Melani, A. G., Loaiza, E. A., Rosa, L. A., de Oliveira, C. M., Levi, J. E., Longatto‑Filho, A."Characterization of topoisomerase II α and minichromosome maintenance protein 2 expression in anal carcinoma". Oncology Letters 13.3 (2017): 1891-1898.
Chicago
Scapulatempo‑Neto, C., Veo, C., Fregnani, J. H., Lorenzi, A., Mafra, A., Melani, A. G., Loaiza, E. A., Rosa, L. A., de Oliveira, C. M., Levi, J. E., Longatto‑Filho, A."Characterization of topoisomerase II α and minichromosome maintenance protein 2 expression in anal carcinoma". Oncology Letters 13, no. 3 (2017): 1891-1898. https://doi.org/10.3892/ol.2017.5650
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