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Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer

  • Authors:
    • Xinfeng Chen
    • Liping Wang
    • Jinyan Liu
    • Lan Huang
    • Li Yang
    • Qun Gao
    • Xiaojuan Shi
    • Jieyao Li
    • Feng Li
    • Zhen Zhang
    • Song Zhao
    • Bin Zhang
    • Pierre Van der Bruggen
    • Yi Zhang
  • View Affiliations / Copyright

    Affiliations: Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Cerebral Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Division of Hematology‑Oncology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA, Ludwig Institute for Cancer Research Brussels Branch, de Duve Institute, Université Catholique de Louvain, 1200 Brussels, Belgium
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1609-1618
    |
    Published online on: February 1, 2017
       https://doi.org/10.3892/ol.2017.5665
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Abstract

Melanoma-associated antigen (MAGE)-A3 and MAGE-C2 are antigens encoded by cancer-germline genes, and have been recognized as potential prognostic biomarkers and attractive targets for immunotherapy in multiple types of cancer. The present study aimed to analyze the clinicopathological significance of MAGE‑A3/C2 expression in non‑small cell lung cancer (NSCLC). The association between MAGE‑A3/C2 mRNA and protein expression, and the pathological characteristics and overall survival of patients with NSCLC was analyzed. In addition, the functional role of MAGE‑A3 in human NSCLC cell line A549 was examined in vitro. MAGE‑A3/C2 mRNA expression was identified in 73% (151/206) and 53% (109/206) of patients with NSCLC, respectively. MAGE‑A3/C2 protein expression was identified in 58% (44/76) and 53% (40/76) of NSCLC cases, respectively. MAGE‑A3 mRNA expression was observed to be associated with smoking history, disease stage and lymph node metastasis. However, no association was identified between MAGE‑C2 mRNA expression and the clinicopathological characteristics of patients with NSCLC. MAGE‑A3/C2‑positive patients had a poorer survival rate compared with MAGE‑A3/C2‑negative patients. Multivariate analysis identified that MAGE‑A3 expression may serve as an independent marker of poor prognosis in patients with NSCLC. Downregulation of MAGE‑A3 mRNA expression in A549 cells resulted in lower migration and colony formation rates, and a higher amount of epithelial marker and lower amount of mesenchymal marker expression compared with the control group. These results indicate that MAGE‑A3 serves a role in NSCLC cell metastasis through the induction of epithelial‑mesenchymal transition. In conclusion, MAGE‑A3 may serve as a diagnostic and prognostic biomarker for patients with NSCLC, due to its association with tumor progression and poor clinical outcome.
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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Wang L, Liu J, Huang L, Yang L, Gao Q, Shi X, Li J, Li F, Zhang Z, Zhang Z, et al: Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer. Oncol Lett 13: 1609-1618, 2017.
APA
Chen, X., Wang, L., Liu, J., Huang, L., Yang, L., Gao, Q. ... Zhang, Y. (2017). Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer. Oncology Letters, 13, 1609-1618. https://doi.org/10.3892/ol.2017.5665
MLA
Chen, X., Wang, L., Liu, J., Huang, L., Yang, L., Gao, Q., Shi, X., Li, J., Li, F., Zhang, Z., Zhao, S., Zhang, B., Van der Bruggen, P., Zhang, Y."Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer". Oncology Letters 13.3 (2017): 1609-1618.
Chicago
Chen, X., Wang, L., Liu, J., Huang, L., Yang, L., Gao, Q., Shi, X., Li, J., Li, F., Zhang, Z., Zhao, S., Zhang, B., Van der Bruggen, P., Zhang, Y."Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer". Oncology Letters 13, no. 3 (2017): 1609-1618. https://doi.org/10.3892/ol.2017.5665
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x
Spandidos Publications style
Chen X, Wang L, Liu J, Huang L, Yang L, Gao Q, Shi X, Li J, Li F, Zhang Z, Zhang Z, et al: Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer. Oncol Lett 13: 1609-1618, 2017.
APA
Chen, X., Wang, L., Liu, J., Huang, L., Yang, L., Gao, Q. ... Zhang, Y. (2017). Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer. Oncology Letters, 13, 1609-1618. https://doi.org/10.3892/ol.2017.5665
MLA
Chen, X., Wang, L., Liu, J., Huang, L., Yang, L., Gao, Q., Shi, X., Li, J., Li, F., Zhang, Z., Zhao, S., Zhang, B., Van der Bruggen, P., Zhang, Y."Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer". Oncology Letters 13.3 (2017): 1609-1618.
Chicago
Chen, X., Wang, L., Liu, J., Huang, L., Yang, L., Gao, Q., Shi, X., Li, J., Li, F., Zhang, Z., Zhao, S., Zhang, B., Van der Bruggen, P., Zhang, Y."Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer". Oncology Letters 13, no. 3 (2017): 1609-1618. https://doi.org/10.3892/ol.2017.5665
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