Prognostic value of intratumoral carbonic anhydrase IX expression in testicular germ cell tumors
Affiliations: Translational Research Unit, Faculty of Medicine, Comenius University, 833 10 Bratislava, Slovak Republic, Department of Pathology, Faculty of Medicine, Comenius University, 811 08 Bratislava, Slovak Republic, Department of Oncology, National Cancer Institute, 833 10 Bratislava, Slovak Republic, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, 845 05 Bratislava, Slovak Republic, First Department of Oncology, Faculty of Medicine, Comenius University and St. Elisabeth Cancer Institute, 812 50 Bratislava, Slovak Republic, Second Department of Oncology, Faculty of Medicine, Comenius University and National Cancer Institute, 833 10 Bratislava, Slovak Republic, Department of Pathology, Slovak Medical University, 833 03 Bratislava, Slovak Republic
- Published online on: February 14, 2017 https://doi.org/10.3892/ol.2017.5745
- Pages: 2177-2185
Copyright: © Kalavska et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...
This article is mentioned in:
Testicular germ cell tumors (TGCTs) represent a highly curable malignancy, however a small proportion of patients fails to be cured with cisplatin‑based chemotherapy. Carbonic anhydrase IX (CA IX) is upregulated by hypoxia in several cancer types and correlates with a poor prognosis. The present translational study evaluated expression and prognostic value of CA IX in TGCTs. Surgical specimens from 228 patients with TGCTs were processed by the tissue microarray method and subjected to immunohistochemistry with the M75 monoclonal antibody. CA IX expression was evaluated in tumors vs. adjacent normal testicular tissues and correlated with clinicopathological characteristics and clinical outcome. CA IX expression was detected in 62 (30.2%) of TGCTs compared to 0 (0%) of normal tissue adjacent to testicular tumor (P<0.001). The highest frequency of the CA IX expression was detected in teratoma (39.0%), followed by seminoma (22.7%), yolk sac tumor (22.2%), embryonal carcinoma (11.9%) and choriocarcinoma (7.7%). None of germ cell neoplasias in situ (GCNIS) exhibited CA IX expression. Patients without the CA IX tumor expression showed significantly better progression‑free survival, but not overall survival, compared to patients with the CA IX expression [hazard ratio (HR), 0.57; 95% CI, 0.32‑1.02; P=0.037 and HR, 0.58; 95% CI, 0.29‑1.16; P=0.088, respectively]. There was no significant correlation between the CA IX expression and clinicopathological variables. The intratumoral CA IX expression can serve as a prognostic marker in the TGCT patients. These results suggest that activation of the hypoxia‑induced pathways may be important in the treatment failure in TGCTs patients.