Open Access

miR-185-3p regulates the invasion and metastasis of nasopharyngeal carcinoma by targeting WNT2B in vitro

  • Authors:
    • Chao Liu
    • Guo Li
    • Shuling Ren
    • Zhongwu Su
    • Yunyun Wang
    • Yongquan Tian
    • Yong Liu
    • Yuanzheng Qiu
  • View Affiliations

  • Published online on: February 24, 2017     https://doi.org/10.3892/ol.2017.5778
  • Pages: 2631-2636
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRs) have been recognised as important regulators of malignant behaviour in different types of human cancer, including nasopharyngeal carcinoma (NPC). A previous study by our group revealed that miR‑185‑3p regulates the radioresistance of NPC cells. The present study aimed to investigate the effect of miR‑185‑3p on NPC invasion and metastasis. Human NPC CNE‑2 and 5‑8F cell lines were transfected with a miR‑185‑3p mimic and miR‑185‑3p inhibitor, respectively, and their effects on the invasion and metastasis of these cells was assessed using a wound healing assay and Matrigel invasion assay. The target gene of miR‑185‑3p, Wnt family member 2B (WNT2B) was silenced in 5-8F cells using siRNA in order to investigate its function in NPC. Data from the present study demonstrated that the expression of miR‑185‑3p was the highest in 5‑8F and lowest in CNE‑2 cells out of a range of NPC cell lines. Following the transfection of miR‑185‑3p mimic into CNE‑2 cells, the wound healing and Matrigel invasion assays indicated that the migration and invasion ability of CNE‑2 cells was significantly reduced compared with the negative control group. In addition, the inhibition of miR‑185‑3p in 5‑8F cells significantly increased the capacity for migration and invasion. Furthermore, silencing WNT2B expression resulted in a significant reduction in the invasion and metastasis in 5‑8F cells. The inhibition of miR‑185‑3p, which promotes invasion and metastasis, could be reversed through the silencing of WNT2B in 5‑8F cells. The results of the present study indicate that miR‑185‑3p mediates the invasion and metastasis of NPC by targeting WNT2B in vitro.

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April 2017
Volume 13 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
APA
Liu, C., Li, G., Ren, S., Su, Z., Wang, Y., Tian, Y. ... Qiu, Y. (2017). miR-185-3p regulates the invasion and metastasis of nasopharyngeal carcinoma by targeting WNT2B in vitro. Oncology Letters, 13, 2631-2636. https://doi.org/10.3892/ol.2017.5778
MLA
Liu, C., Li, G., Ren, S., Su, Z., Wang, Y., Tian, Y., Liu, Y., Qiu, Y."miR-185-3p regulates the invasion and metastasis of nasopharyngeal carcinoma by targeting WNT2B in vitro". Oncology Letters 13.4 (2017): 2631-2636.
Chicago
Liu, C., Li, G., Ren, S., Su, Z., Wang, Y., Tian, Y., Liu, Y., Qiu, Y."miR-185-3p regulates the invasion and metastasis of nasopharyngeal carcinoma by targeting WNT2B in vitro". Oncology Letters 13, no. 4 (2017): 2631-2636. https://doi.org/10.3892/ol.2017.5778