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Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells

  • Authors:
    • Jun-Bao Gu
    • Xue-Bin Bao
    • Zhao Ma
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China
    Copyright: © Gu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 618-622
    |
    Published online on: May 15, 2017
       https://doi.org/10.3892/ol.2017.6171
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Abstract

The present study investigated the expression of miR-21 in MGC803 gastric cancer cells and its effects on Bcl-2 expression and cell proliferation, apoptosis, and invasion. In total 50 patients were recruited with gastric cancer who were admitted to the Henan Province People's Hospital. The samples of gastric cancer and the adjacent normal tissues were collected after surgery. We found that mRNA levels of miR-21 and Bcl-2 were significantly elevated in tumor tissues compared to control tissue. The expression of Bcl-2 protein was also elevated in cancerous tissue. This high expression of Bcl-2 was associated with clinical stage, lymph node metastasis, and tumor differentiation degree. Inhibition of miR-21 reduced the levels of miR-21 and Bcl-2 in MGC803 cells, and lowered cell proliferation and invasiveness. These results indicate that miR-21 and Bcl-2 may participate in the occurrence and development of gastric adenocarcinoma, suggesting their potential role as biomarkers and therapeutic targets.
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1 

Lee JH, Kim JG, Jung HK, Kim JH, Jeong WK, Jeon TJ, Kim JM, Kim YI, Ryu KW, Kong SH, et al: Clinical practice guidelines for gastric cancer in Korea: An evidence-based approach. J Gastric Cancer. 14:87–104. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Long ZW, Yu HM, Wang YN, Liu D, Chen YZ, Zhao YX and Bai L: Association of IL-17 polymorphisms with gastric cancer risk in Asian populations. World J Gastroenterol. 21:5707–5718. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Chen XZ, Chen H, Castro FA, Hu JK and Brenner H: Epstein-Barr virus infection and gastric cancer: a systematic review. Medicine (Baltimore). 94:e7922015. View Article : Google Scholar : PubMed/NCBI

4 

Kanaan Z, Rai SN, Eichenberger MR, Roberts H, Keskey B, Pan J and Galandiuk S: Plasma miR-21: a potential diagnostic marker of colorectal cancer. Ann Surg. 256:544–551. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Li T, Li RS, Li YH, Zhong S, Chen YY, Zhang CM, Hu MM and Shen ZJ: miR-21 as an independent biochemical recurrence predictor and potential therapeutic target for prostate cancer. J Urol. 187:1466–1472. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Liu XG, Zhu WY, Huang YY, Ma LN, Zhou SQ, Wang YK, Zeng F, Zhou JH and Zhang YK: High expression of serum miR-21 and tumor miR-200c associated with poor prognosis in patients with lung cancer. Med Oncol. 29:618–626. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Han M, Wang Y, Liu M, Bi X, Bao J, Zeng N, Zhu Z, Mo Z, Wu C and Chen X: MiR-21 regulates epithelial-mesenchymal transition phenotype and hypoxia-inducible factor-1α expression in third-sphere forming breast cancer stem cell-like cells. Cancer Sci. 103:1058–1064. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Lakomy R, Sana J, Hankeova S, Fadrus P, Kren L, Lzicarova E, Svoboda M, Dolezelova H, Smrcka M, Vyzula R, et al: MiR-195, miR-196b, miR-181c, miR-21 expression levels and O-6-methylguanine-DNA methyltransferase methylation status are associated with clinical outcome in glioblastoma patients. Cancer Sci. 102:2186–2190. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Lu QL, Abel P, Foster CS and Lalani EN: bcl-2: role in epithelial differentiation and oncogenesis. Hum Pathol. 27:102–110. 1996. View Article : Google Scholar : PubMed/NCBI

10 

Luanpitpong S, Chanvorachote P, Stehlik C, Tse W, Callery PS, Wang L and Rojanasakul Y: Regulation of apoptosis by Bcl-2 cysteine oxidation in human lung epithelial cells. Mol Biol Cell. 24:858–869. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Flangea C, Potencz E, Mihăescu R, Gîju S and Anghel A: Bcl-2 expression in Hodgkin's lymphoma progression. Rom J Morphol Embryol. 49:357–363. 2008.PubMed/NCBI

12 

Manne U, Weiss HL and Grizzle WE: Bcl-2 expression is associated with improved prognosis in patients with distal colorectal adenocarcinomas. Int J Cancer. 89:423–430. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Zhou XL and Wang M: Expression levels of survivin, Bcl-2, and KAI1 proteins in cervical cancer and their correlation with metastasis. Genet Mol Res. 14:17059–17067. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Hajnóczky G, Csordás G, Das S, Garcia-Perez C, Saotome M, Sinha Roy S and Yi M: Mitochondrial calcium signalling and cell death: approaches for assessing the role of mitochondrial Ca2+ uptake in apoptosis. Cell Calcium. 40:553–560. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Shimamoto S, Tsuchiya M, Yamaguchi F, Kubota Y, Tokumitsu H and Kobayashi R: Ca2+/S100 proteins inhibit the interaction of FKBP38 with Bcl-2 and Hsp90. Biochem J. 458:141–152. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Li Y, Yimamu M, Wang X, Zhang X, Mao M, Fu L, Aisimitula A, Nie Y and Huang Q: Addition of rituximab to a CEOP regimen improved the outcome in the treatment of non-germinal center immunophenotype diffuse large B cell lymphoma cells with high Bcl-2 expression. Int J Hematol. 99:79–86. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Simsek EN and Uysal T: In vitro investigation of cytotoxic and apoptotic effects of Cynara L. species in colorectal cancer cells. Asian Pac J Cancer Prev. 14:6791–6795. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Luo KW, Ko CH, Yue GGL, Lee JK, Li KK, Lee M, Li G, Fung KP, Leung PC and Lau CB: Green tea (Camellia sinensis) extract inhibits both the metastasis and osteolytic components of mammary cancer 4T1 lesions in mice. J Nutr Biochem. 25:395–403. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Hu CJ, Zhou L and Cai Y: Dihydroartemisinin induces apoptosis of cervical cancer cells via upregulation of RKIP and downregulation of bcl-2. Cancer Biol Ther. 15:279–288. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Pushkarev VM, Kovzun OI, Pushkarev VV and Tronko M: Biochemical effects of combined action of γ-irradiation and paclitaxel on anaplastic thyroid cancer cells. Ukr Biokhim Zh (1999). 85:51–61. 2013.PubMed/NCBI

21 

Banzhaf-Strathmann J and Edbauer D: Good guy or bad guy: the opposing roles of microRNA 125b in cancer. Cell Commun Signal. 12:302014. View Article : Google Scholar : PubMed/NCBI

22 

Lim L, Balakrishnan A, Huskey N, Jones KD, Jodari M, Ng R, Song G, Riordan J, Anderton B, Cheung ST, et al: MicroRNA-494 within an oncogenic microRNA megacluster regulates G1/S transition in liver tumorigenesis through suppression of mutated in colorectal cancer. Hepatology. 59:202–215. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Melo SA, Sugimoto H, O'Connell JT, Kato N, Villanueva A, Vidal A, Qiu L, Vitkin E, Perelman LT, Melo CA, et al: Cancer exosomes perform cell-independent microRNA biogenesis and promote tumorigenesis. Cancer Cell. 26:707–721. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Kasinski AL, Kelnar K, Stahlhut C, Orellana E, Zhao J, Shimer E, Dysart S, Chen X, Bader AG and Slack FJ: A combinatorial microRNA therapeutics approach to suppressing non-small cell lung cancer. Oncogene. 34:3547–3555. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Chan JA, Krichevsky AM and Kosik KS: MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res. 65:6029–6033. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Meng F, Henson R, Lang M, Wehbe H, Maheshwari S, Mendell JT, Jiang J, Schmittgen TD and Patel T: Involvement of human micro-RNA in growth and response to chemotherapy in human cholangiocarcinoma cell lines. Gastroenterology. 130:2113–2129. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Lund E, Güttinger S, Calado A, Dahlberg JE and Kutay U: Nuclear export of microRNA precursors. Science. 303:95–98. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Asangani IA, Rasheed SAK, Nikolova DA, Leupold JH, Colburn NH, Post S and Allgayer H: MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer. Oncogene. 27:2128–2136. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Iorio MV, Ferracin M, Liu CG, Veronese A, Spizzo R, Sabbioni S, Magri E, Pedriali M, Fabbri M, Campiglio M, et al: MicroRNA gene expression deregulation in human breast cancer. Cancer Res. 65:7065–7070. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Frankel LB, Christoffersen NR, Jacobsen A, Lindow M, Krogh A and Lund AH: Programmed cell death 4 (PDCD4) is an important functional target of the microRNA miR-21 in breast cancer cells. J Biol Chem. 283:1026–1033. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Gu J, Bao X and Ma Z: Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells. Oncol Lett 15: 618-622, 2018.
APA
Gu, J., Bao, X., & Ma, Z. (2018). Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells. Oncology Letters, 15, 618-622. https://doi.org/10.3892/ol.2017.6171
MLA
Gu, J., Bao, X., Ma, Z."Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells". Oncology Letters 15.1 (2018): 618-622.
Chicago
Gu, J., Bao, X., Ma, Z."Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells". Oncology Letters 15, no. 1 (2018): 618-622. https://doi.org/10.3892/ol.2017.6171
Copy and paste a formatted citation
x
Spandidos Publications style
Gu J, Bao X and Ma Z: Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells. Oncol Lett 15: 618-622, 2018.
APA
Gu, J., Bao, X., & Ma, Z. (2018). Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells. Oncology Letters, 15, 618-622. https://doi.org/10.3892/ol.2017.6171
MLA
Gu, J., Bao, X., Ma, Z."Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells". Oncology Letters 15.1 (2018): 618-622.
Chicago
Gu, J., Bao, X., Ma, Z."Effects of miR-21 on proliferation and apoptosis in human gastric adenocarcinoma cells". Oncology Letters 15, no. 1 (2018): 618-622. https://doi.org/10.3892/ol.2017.6171
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