Relationship between miR-21 and miR-182 levels in peripheral blood and gastric cancer tissue

Wang,Xiaodong; Wang,Ran; Li,Fenghuan; Wu,Yuan; Liu,Yu; Zhang,Wenfang

doi:10.3892/ol.2017.6280

August-2017 Volume 14 Issue 2

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August-2017 Volume 14 Issue 2

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Relationship between miR-21 and miR-182 levels in peripheral blood and gastric cancer tissue

Authors:
- Xiaodong Wang
- Ran Wang
- Fenghuan Li
- Yuan Wu
- Yu Liu
- Wenfang Zhang
View Affiliations / Copyright

Affiliations: Department of Clinical Laboratory, 254th Hospital of PLA, Tianjin 300142, P.R. China, Department of Intensive Care Unit, Nankai Hospital Affiliated to Tianjin Medical University, Tianjin 300142, P.R. China, Department of Clinical Laboratory, Tumor Hospital Affiliated to Tianjin Medical University, Tianjin 300142, P.R. China

Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 1427-1432
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Published online on: May 30, 2017

https://doi.org/10.3892/ol.2017.6280
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Abstract

The relationship between miR-21 and miR-182 gene expression in peripheral blood and gastric cancer tissue was investigated, exploring the relationship between the levels of miR-21 and miR-182 and prognosis of gastric cancer patients, and determining the effects of these two genes on the growth and migration of gastric cancer cells. Fifty gastric cancer patients who were treated in the 254th Hospital of PLA, from July 2012 to July 2014 were selected. Peripheral blood samples were drawn from patients, and 50 healthy subjects were studied as controls. The levels of the miR-21 and miR-182 genes were detected by semi-quantitative PCR, and the correlation between miR-21 and miR-182 expression and clinicopathological features was explored. Moreover, the effects of miR-21 and miR-182 expression on the survival time and prognosis of patients were investigated. siRNA was used to downregulate miR-21 and miR-182 gene expression in MGC-803 gastric cancer cells, and MTT and Transwell assays were conducted. As a result, the relative expression levels of miR-21 and miR-182 in peripheral blood of gastric cancer patients were significantly higher than in healthy subjects (p<0.01) and the relative expression of miR-182 was closely related to the clinicopathological features of gastric cancer patients (p<0.05); high expression of miR-21 and miR-182 was associated with reduced survival time of patients (p<0.05); MGC-803 cells with low expression of miR-21 and miR-182 were analyzed, showing that miR-182 promoted cell proliferation and migration (p<0.01). In conclusion, the relative levels of miR-21 and miR-182 in peripheral blood of patients with gastric cancer are significantly increased; low expression of miR-182 can significantly reduce the proliferation and migration of gastric cancer cells. Moreover, miR-182 expression, which is closely related to the clinicopathological features of gastric cancer, can serve as a target for the clinical treatment of gastric cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Relationship between miR-21 and miR-182 levels in peripheral blood and gastric cancer tissue

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