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Article

Association between protocadherin 8 promoter hypermethylation and the pathological status of prostate cancer

  • Authors:
    • Peng Zhang
    • Hui Wang
    • Jianming Wang
    • Qingzuo Liu
    • Yongqiang Wang
    • Fan Feng
    • Lei Shi
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China
  • Pages: 1657-1664
    |
    Published online on: May 31, 2017
       https://doi.org/10.3892/ol.2017.6282
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Abstract

Promoter hypermethylation of tumor suppressor genes has been confirmed to serve a pivotal role in tumorigenesis. Protocadherin 8 (PCDH8), a novel tumor suppressor gene, has been reported to be inactivated by promoter hypermethylation a number of cancer types, including bladder cancer and renal cell carcinoma. The aim of the present study was to investigate the occurrence of PCDH8 hypermethylation in prostate cancer and its potential as a novel biomarker of prostate cancer. The transcriptional levels of PCDH8 were examined by quantitative polymerase chain reaction (PCR) in 82 prostate cancer tissues as well as 30 prostate hyperplasia tissues, and verified the protein level by western blot analysis of representative samples. PCDH8 expression levels were found to be reduced to 0.30±0.10 in 70.7% (58/82) of prostate cancer tissues. To identify the possible reason for mRNA downregulation, the methylation status of the PCDH8 promoter was assessed in prostate cancer tissues and prostate hyperplasia tissues by methylation‑specific PCR (MSP). A total of 47 prostate cancer patients who exhibited reduced PCDH8 expression (57.3%; 47/82) also showed promoter hypermethylation (47/58). None of the samples (0/30) in the benign prostate hyperplasia group were positive on MSP. Furthermore, the associations between the methylation status of the PCDH8 promoter and various clinicopathological features of prostate cancer were analyzed, revealing that the methylation status of PCDH8 was closely associated with tumor size, tumor shape (papillary/non-papillary), tumor stage and tumor grade (all P<0.05), while there were no correlations with the age of the patients or the number of tumors (P>0.05). Additionally, patients with hypermethylation of the PCDH8 gene promoter had a relapse rate of 36.17% and a mortality rate of 29.79%, which were significantly higher than the hypermethylation-negative patients (P<0.05), indicating a poorer prognosis. Therefore, the methylation status of the PCDH8 gene in prostate cancer may be an important marker for use in the early diagnosis and prediction of prognosis in prostate cancer.
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1 

Javanmard B, Haddad A Hassanzadeh, Yaghoobi M and Lotfi B: Diode laser ablation of prostate and channel transurethral resection of prostate in patients with prostate cancer and bladder outlet obstruction symptoms. Urol J. 11:1788–1792. 2014.PubMed/NCBI

2 

Park JY: Promoter hypermethylation in prostate cancer. Cancer Control. 17:245–255. 2010.PubMed/NCBI

3 

Deaton AM and Bird A: CpG islands and the regulation of transcription. Genes Dev. 25:1010–1022. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Morris MR, Ricketts CJ, Gentle D, McRonald F, Carli N, Khalili H, Brown M, Kishida T, Yao M, Banks RE, et al: Genome-wide methylation analysis identifies epigenetically inactivated candidate tumour suppressor genes in renal cell carcinoma. Oncogene. 30:1390–1401. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Yu JS, Koujak S, Nagase S, Li CM, Su T, Wang X, Keniry M, Memeo L, Rojtman A, Mansukhani M, et al: PCDH8, the human homolog of PAPC, is a candidate tumor suppressor of breast cancer. Oncogene. 27:4657–4665. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Zhang D, Zhao W, Liao X, Bi T, Li H and Che X: !, !Frequent silencing of protocadherin 8 by promoter methylation, a candidate tumor suppressor for human gastric cancer. Oncol Rep. 28:1785–1791. 2012.PubMed/NCBI

7 

Lin YL, Ma JH, Luo XL, Guan TY and Li ZG: Clinical significance of protocadherin-8 (PCDH8) promoter methylation in bladder cancer. J Int Med Res. 41:48–54. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Welch HG and Albertsen PC: Prostate cancer diagnosis and treatment after the introduction of prostate-specific antigen screening: 1986–2005. J Ntl Cancer Inst. 101:1325–1329. 2009. View Article : Google Scholar

9 

Partin AW, Mangold LA, Lamm DM, Walsh PC, Epstein JI and Pearson JD: Contemporary update of prostate cancer staging nomograms (Partin Tables) for the new millennium. Urology. 58:843–848. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Ugolkov AV, Eisengart LJ, Luan C and Yang XJ: Expression analysis of putative stem cell markers in human benign and malignant prostate. Prostate. 71:18–25. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Barfeld SJ, East P, Zuber V and Mills IG: Meta-analysis of prostate cancer gene expression data identifies a novel discriminatory signature enriched for glycosylating enzymes. BMC Med Genomics. 7:5132014. View Article : Google Scholar : PubMed/NCBI

12 

Tong SJ, Liu J, Wang X and Qu LX: microRNA-181 promotes prostate cancer cell proliferation by regulating DAX-1 expression. Exp Ther Med. 8:1296–1300. 2014.PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Frank SJ, Levy LB, van Vulpen M, Crook J, Sylvester J, Grimm P, Pugh TJ and Swanson DA: Outcomes after prostate brachytherapy are even better than predicted. Cancer. 118:839–847. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Monma F, Hozumi Y, Ikematsu S, Kawaguchi M, Kadomatsu K and Suzuki T: Expression of midkine in normal human skin, dermatitis and neoplasms: Association with differentiation of keratinocytes. J Dermatol. 40:980–986. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Tangen CM, Faulkner JR, Crawford ED, Thompson IM, Hirano D, Eisenberger M and Hussain M: Ten-year survival in patients with metastatic prostate cancer. Clin Prostate Cancer. 2:41–45. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Jennbacken K, Vallbo C, Wang W and Damber JE: Expression of vascular endothelial growth factor C (VEGF-C) and VEGF receptor-3 in human prostate cancer is associated with regional lymph node metastasis. Prostate. 65:110–116. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Sheyhidin I, Hasim A, Zheng F and Ma H: Epigenetic changes within the promoter regions of antigen processing machinery family genes in Kazakh primary esophageal squamous cell carcinoma. Asian Pac J Cancer Prev. 15:10299–10306. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Yu JS, Koujak S, Nagase S, Li CM, Su T, Wang X, Keniry M, Memeo L, Rojtman A, Mansukhani M, et al: PCDH8, the human homolog of PAPC, is a candidate tumor suppressor of breast cancer. Oncogene. 27:4657–4665. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Bray NJ, Kirov G, Owen RJ, Jacobsen NJ, Georgieva L, Williams HJ, Norton N, Spurlock G, Jones S, Zammit S, et al: Screening the human protocadherin 8 (PCDH8) gene in schizophrenia. Genes Brain Behav. 1:187–191. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Létoquart J, Huvelle E, Wacheul L, Bourgeois G, Zorbas C, Graille M, Heurgué-Hamard V and Lafontaine DL: Structural and functional studies of Bud23-Trm112 reveal 18S rRNA N7-G1575 methylation occurs on late 40S precursor ribosomes. Proc Natl Acad Sci USA. 111:pp. E5518–E5526. 2014; View Article : Google Scholar : PubMed/NCBI

22 

Heichman KA and Warren JD: DNA methylation biomarkers and their utility for solid cancer diagnostics. Clin Chem Lab Med. 50:1707–1721. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Redshaw N, Huggett JF, Taylor MS, Foy CA and Devonshire AS: Quantification of epigenetic biomarkers: An evaluation of established and emerging methods for DNA methylation analysis. BMC Genomics. 15:11742014. View Article : Google Scholar : PubMed/NCBI

24 

LePage DP, Jernigan KK and Bordenstein SR: The relative importance of DNA methylation and Dnmt2-mediated epigenetic regulation on Wolbachia densities and cytoplasmic incompatibility. PeerJ. 2:e6782014. View Article : Google Scholar : PubMed/NCBI

25 

Peng L, Wei H and Liren L: Promoter methylation assay of SASH1 gene in hepatocellular carcinoma. J BUON. 19:1041–1047. 2014.PubMed/NCBI

26 

Jeffries MA and Sawalha AH: Autoimmune disease in the epigenetic era: How has epigenetics changed our understanding of disease and how can we expect the field to evolve? Expert Rev Clin Immunol. 11:45–58. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Sircar K, Huang H, Hu L, Cogdell D, Dhillon J, Tzelepi V, Efstathiou E, Koumakpayi IH, Saad F, Luo D, et al: Integrative molecular profiling reveals asparagine synthetase is a target in castration-resistant prostate cancer. Am J Pathol. 180:895–903. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Yi JM, Dhir M, Guzzetta AA, Iacobuzio-Donahue CA, Heo K, Yang KM, Suzuki H, Toyota M, Kim HM and Ahuja N: DNA methylation biomarker candidates for early detection of colon cancer. Tumour Biol. 33:363–372. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang P, Wang H, Wang J, Liu Q, Wang Y, Feng F and Shi L: Association between protocadherin 8 promoter hypermethylation and the pathological status of prostate cancer. Oncol Lett 14: 1657-1664, 2017.
APA
Zhang, P., Wang, H., Wang, J., Liu, Q., Wang, Y., Feng, F., & Shi, L. (2017). Association between protocadherin 8 promoter hypermethylation and the pathological status of prostate cancer. Oncology Letters, 14, 1657-1664. https://doi.org/10.3892/ol.2017.6282
MLA
Zhang, P., Wang, H., Wang, J., Liu, Q., Wang, Y., Feng, F., Shi, L."Association between protocadherin 8 promoter hypermethylation and the pathological status of prostate cancer". Oncology Letters 14.2 (2017): 1657-1664.
Chicago
Zhang, P., Wang, H., Wang, J., Liu, Q., Wang, Y., Feng, F., Shi, L."Association between protocadherin 8 promoter hypermethylation and the pathological status of prostate cancer". Oncology Letters 14, no. 2 (2017): 1657-1664. https://doi.org/10.3892/ol.2017.6282
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang P, Wang H, Wang J, Liu Q, Wang Y, Feng F and Shi L: Association between protocadherin 8 promoter hypermethylation and the pathological status of prostate cancer. Oncol Lett 14: 1657-1664, 2017.
APA
Zhang, P., Wang, H., Wang, J., Liu, Q., Wang, Y., Feng, F., & Shi, L. (2017). Association between protocadherin 8 promoter hypermethylation and the pathological status of prostate cancer. Oncology Letters, 14, 1657-1664. https://doi.org/10.3892/ol.2017.6282
MLA
Zhang, P., Wang, H., Wang, J., Liu, Q., Wang, Y., Feng, F., Shi, L."Association between protocadherin 8 promoter hypermethylation and the pathological status of prostate cancer". Oncology Letters 14.2 (2017): 1657-1664.
Chicago
Zhang, P., Wang, H., Wang, J., Liu, Q., Wang, Y., Feng, F., Shi, L."Association between protocadherin 8 promoter hypermethylation and the pathological status of prostate cancer". Oncology Letters 14, no. 2 (2017): 1657-1664. https://doi.org/10.3892/ol.2017.6282
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