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Role and mechanism of decitabine combined with tyrosine kinase inhibitors in advanced chronic myeloid leukemia cells

  • Authors:
    • Li‑Cai Jiang
    • Jian‑Min Luo
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1295-1302
    |
    Published online on: June 6, 2017
       https://doi.org/10.3892/ol.2017.6318
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Abstract

Patients with advanced chronic myeloid leukemia (CML) have a poor prognosis, with the use of tyrosine kinase inhibitors (TKIs) to treat CML demonstrating poor results. The results of the present study revealed that, following Cell Counting Kit-8 analysis, treatment of K562 cells with decitabine (DAC) combined with TKIs exhibits synergic effects. Co‑immunoprecipitation indicated that tyrosine‑protein phosphatase non‑receptor type 6 (SHP‑1) and BCR‑ABL fusion protein (BCR‑ABL) (p210) form a complex in the K562 cell line, and in the primary cells derived from patients with CML. These results suggested that SHP‑1 serves a role in regulating the tyrosine kinase activity of BCR‑ABL (p210). In addition, SHP‑1 expression increased, while BCR‑ABL expression decreased in the group treated with DAC and TKIs combined group compared with the TKI monotherapy group. Treatment with imatinib (IM) demonstrated no effect on SHP‑1 methylation in the K562 cell line; however, the methylation of SHP‑1 was not determined in the combined IM and DAC therapy group. Treatment with DAC demonstrated the ability to activate the expression of silenced SHP‑1 through demethylation, thus decreasing BCR-ABL tyrosine kinase activity, resulting in an improved therapeutic effect on CML.
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1 

National Comprehensive Cancer Network, . NCCN Clinical Practice Guidelines in Oncology: Chronic Myelogenous Leukemia, V.2.[DB/OL].(2007-10-25). 2008.

2 

Druker BJ and Lee SJ: Chronic myelogenous leukemia. Cancer: Principles and Practice of Oncology. 2007.

3 

Incidence survey of leukemia in China, . Chinese Epidemiologic Study Group of Leukemia and Aplastic Anemia. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 14:12–19. 1992.(In Chinese). PubMed/NCBI

4 

Goldman JM and Melo JV: Chronic myeloid leukemia-advances in biology and new approaches to treatment. N Engl J Med. 349:1451–1464. 2003. View Article : Google Scholar : PubMed/NCBI

5 

McWhirter JR and Wang JY: An actin-binding function contributes to transformation by the Bcr-Abl oncoprotein of Philadelphia chromosome-positive human leukemias. EMBO J. 12:1533–1546. 1993.PubMed/NCBI

6 

van Etten RA, Jackson PK, Baltimore D, Sanders MC, Matsudaira PT and Janmey PA: The COOH terminus of the c-Abl tyrosine kinase contains distinct F- and G-actin binding domains with bundling activity. J Cell Biol. 124:325–340. 1994. View Article : Google Scholar : PubMed/NCBI

7 

Foroni L, Gerrard G, Nna E, Khorashad JS, Stevens D, Swale B, Milojkovic D, Reid A, Goldman J and Marin D: Technical aspects and clinical applications of measuring BCR-ABL1 transcripts number in chronic myeloid leukemia. Am J Hematol. 84:517–522. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Hackanson B and Daskalakis M: Decitabine. Recent Results Cancer Res. 201:269–297. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Kantarjian HM, O'Brien SM, Keating M, Beran M, Estey E, Giralt S, Kornblau S, Rios MB, de Vos D and Talpaz M: Results of decitabine therapy in the accelerated and blastic phases of chronic myelogenous leukemia. Leukemia. 11:1617–1620. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Sacchi S, Kantarjian HM, O'Brien S, Cortes J, Rios MB, Giles FJ, Beran M, Koller CA and Keating MJ: Chronic myelogenous leukemia in nonlymphoidblastic phase: Analysis of the results of first salvage therapy with three different treatment approaches for 162 patients. Cancer. 86:2632–2641. 1999. View Article : Google Scholar : PubMed/NCBI

11 

Kantarjian HM, O'Brien S, Cortes J, Giles FJ, Faderl S, Issa JP, Garcia-Manero G, Rios MB, Shan J, Andreeff M, et al: Results of decitabine (5-aza-2-deoxycytidine) therapy in 130 patients with chronic myelogenousleukemia. Cancer. 98:522–528. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Issa JP, Garcia-Manero G, Giles FJ, Mannari R, Thomas D, Faderl S, Bayar E, Lyons J, Rosenfeld CS, Cortes J and Kantarjian HM: Phase 1 study of low-dose prolonged exposure schedules of the hypomethylating agent 5-aza-2-deoxycytidine (decitabine) in hematopoietic malignancies. Blood. 103:1635–1640. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Issa JP, Gharibyan V, Cortes J, Jelinek J, Morris G, Verstovsek S, Talpaz M, Garcia-Manero G and Kantarjian HM: Phase II study of low-dose decitabine in patients with chronic myelogenous leukemia resistant to imatinib mesylate. J Clin Oncol. 23:3948–3956. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Wu C, Sun M, Liu L and Zhou GW: The function of the protein tyrosine phosphatase SHP-1 in cancer. Gene. 306:1–12. 2003. View Article : Google Scholar : PubMed/NCBI

15 

Oka T, Yoshino T, Hayashi K, Ohara N, Nakanishi T, Yamaa Y, Hiraki A, Sogawa CA, Kondo E, Teramoto N, et al: Reduction of hematopoietic cell specific tyrosine phosphatase SHP-1 gene expression in natural killer cell lymphoma and various types of lymphom as/leukemia. Combination analysic with cDNA expression array and tissue micrearray. Am J Patho. 159:1495–1505. 2001. View Article : Google Scholar

16 

Amin HM, Hoshino K, Yang H, Lin Q, Lai R and Garcia-Manero G: Decreased expression level of SH2 domain-containing protein tyrosine phosphatase-1 (Shp1) is associated with progression of chronic myeloid leukaemia. J Pathol. 212:402–410. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Zhang Q, Wang HY, Marzec M, Raghunath PN, Nagasawa T and Wasik MA: STAT3- and DNA methyltransferase 1-mediated epigenetic silencing of SHP-1 tyrosine phosphatase tumor suppressor gene in malignant T lymphocyte. Proc Natl Acad Sci USA. 102:pp. 6948–6953. 2005; View Article : Google Scholar : PubMed/NCBI

18 

Uhm KO, Lee ES, Lee YM, Park JS, Kim SJ, Kim BS, Kim HS and Park SH: Differential methylation pattern of ID4, SFRP1, and SHP1 between acute myeloid leukemia and chronic myeloid leukemia. J Korean Med Sci. 24:493–497. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Chim CS, Fung TK, Cheung WC, Liang R and Kwong YL: SOCS1 and SHP1 hypermethylation in multiple myeloma: Implications for epigenetic activation of the Jak/STAT pathway. Blood. 103:4630–4635. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Kim JH, Choi YD, Lee JS, Lee JH, Nam JH and Choi C: Assessment of DNA methylation for the detection of cervical neoplasiain liquid-based cytology specimens. Gynecol Oncol. 116:99–104. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Xu SB, Liu XH, Li BH, Zhang Y, Yuan J, Yuan Q, Li PD, Yang XZ, Li F and Zhang WJ: DNA methylation regulates constitutive expression of Stat6 regulatory genes SOCS-1 and SHP-1 in colon cancer cells. J Cancer Res Clin Oncol. 135:1791–1798. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Liedtke M, Pandey P, Kumar S, Kharbanda S and Kufe D: Regulation of Bcr-Abl-induced SAP kinase activity and transformation by the SHPTP1 protein tyrosine phosphatase. Oncogene. 17:1889–1892. 1998. View Article : Google Scholar : PubMed/NCBI

23 

Cramer K, Nieborowska-Skorska M, Koptyra M, Slupianek A, Penserga ET, Eaves CJ, Aulitzky W and Skorski T: BCR/ABL and other kinases from chronic myeloproliferative disorders stimulate single-strand annealing, an unfaithful DNA double-strand break repair. Cancer Res. 68:6884–6888. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Baccarani M, Deininger MW, Rosti G, Hochhaus A, Soverini S, Apperley JF, Cervantes F, Clark RE, Cortes JE, Guilhot F, et al: European LeukemiaNet recommendations for the management of chronic myeloid leukemia: 2013. Blood. 122:872–884. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Oka T, Ouchida M, Koyama M, Ogama Y, Takada S, Nakatani Y, Tanaka T, Yoshino T, Hayashi K, Ohara N, et al: Gene silencing of the tyrosine phosphatase SHP1 gene by aberrant methylation in leukemias/lymphomas. Cancer Res. 62:6390–6394. 2002.PubMed/NCBI

27 

Soriano AF, Helfrich B, Chan DC, Heasley LE, Bunn PA Jr and Chou TC: Synergistic effects of new chemopreventive agents and conventional cytotoxic agents against human lung cancer cell lines. Cancer Res. 59:6178–6184. 1999.PubMed/NCBI

28 

Lozzio CB and Lozzio BB: Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood. 45:321–334. 1975.PubMed/NCBI

29 

Momparler RL, Côté S and Eliopoulos N: Pharmacological approach for optimization of the dose schedule of 5-Aza-2-deoxycytidine (Decitabine) for the therapy of leukemia. Leukemia. 11 Suppl 1:S1–S6. 1997.PubMed/NCBI

30 

Savage DG and Antman KH: Imatinib mesylate-a new oral targeted therapy. N Eng J Med. 346:683–693. 2002. View Article : Google Scholar

31 

Tonks NK and Neel BG: From form to function: Signaling by protein tyrosine phosphatases. Cell. 87:365–368. 1996. View Article : Google Scholar : PubMed/NCBI

32 

Bruecher-Encke B, Griffin JD, Neel BG and Lorenz U: Role of the tyrosine phosphatase SHP-1 in K562 cell differentiation. Leukemia. 15:1424–1432. 2001. View Article : Google Scholar : PubMed/NCBI

33 

Esposito N, Colavita I, Quintarelli C, Sica AR, Peluso AL, Luciano L, Picardi M, Del Vecchio L, Buonomo T, Hughes TP, et al: SHP-1 expression accounts for resistance to imatinib treatment in Philadelphia chromosome-positive cells derived from patients with chronic myeloid leukemia. Blood. 118:3634–3644. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jiang LC and Luo JM: Role and mechanism of decitabine combined with tyrosine kinase inhibitors in advanced chronic myeloid leukemia cells. Oncol Lett 14: 1295-1302, 2017.
APA
Jiang, L., & Luo, J. (2017). Role and mechanism of decitabine combined with tyrosine kinase inhibitors in advanced chronic myeloid leukemia cells. Oncology Letters, 14, 1295-1302. https://doi.org/10.3892/ol.2017.6318
MLA
Jiang, L., Luo, J."Role and mechanism of decitabine combined with tyrosine kinase inhibitors in advanced chronic myeloid leukemia cells". Oncology Letters 14.2 (2017): 1295-1302.
Chicago
Jiang, L., Luo, J."Role and mechanism of decitabine combined with tyrosine kinase inhibitors in advanced chronic myeloid leukemia cells". Oncology Letters 14, no. 2 (2017): 1295-1302. https://doi.org/10.3892/ol.2017.6318
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang LC and Luo JM: Role and mechanism of decitabine combined with tyrosine kinase inhibitors in advanced chronic myeloid leukemia cells. Oncol Lett 14: 1295-1302, 2017.
APA
Jiang, L., & Luo, J. (2017). Role and mechanism of decitabine combined with tyrosine kinase inhibitors in advanced chronic myeloid leukemia cells. Oncology Letters, 14, 1295-1302. https://doi.org/10.3892/ol.2017.6318
MLA
Jiang, L., Luo, J."Role and mechanism of decitabine combined with tyrosine kinase inhibitors in advanced chronic myeloid leukemia cells". Oncology Letters 14.2 (2017): 1295-1302.
Chicago
Jiang, L., Luo, J."Role and mechanism of decitabine combined with tyrosine kinase inhibitors in advanced chronic myeloid leukemia cells". Oncology Letters 14, no. 2 (2017): 1295-1302. https://doi.org/10.3892/ol.2017.6318
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