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Article Open Access

Analysis of salivary microRNA expression profiles and identification of novel biomarkers in esophageal cancer

  • Authors:
    • Jiang Du
    • Lin Zhang
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    Affiliations: Department of Thoracic Surgery, Chinese Medical University Affiliated No. 1 Hospital, Shenyang, Liaoning 110001, P.R. China
    Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1387-1394
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    Published online on: June 7, 2017
       https://doi.org/10.3892/ol.2017.6328
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Abstract

MicroRNAs (miRNAs/miRs) regulate the expression of target genes and are considered to be associated with human cancer. The aim of the present study was to screen novel miRNA biomarkers in esophageal cancer (EC). The miRNA expression profile GSE41268 was extracted from Gene Expression Omnibus database, and differentially expressed miRNAs between whole saliva samples from patients with EC and healthy controls were identified using the Linear Models for Microarray Data package. Then, the targets of these miRNAs were screened using the miRecords database and used to construct the regulatory network. Gene ontology and pathway enrichment analyses were performed for the target genes of differentially expressed miRNAs to predict their potential functions. A total of 18 differentially expressed miRNAs were identified in saliva samples from patients with EC, and 43 validated target genes corresponding to 7 upregulated miRNAs were identified. Then, 6 miRNAs (miR‑144, miR‑451, miR‑98, miR‑10b, miR‑486‑5p and miR‑363) and their target genes were used to construct a regulatory network. Within the network, miR‑144 may target Notch homolog 1, fibrinogen α chain and fibrinogen β chain; miR‑451 may regulate murine thymoma viral oncogene homolog 1, matrix metalloproteinase (MMP)9 and MMP2; miR‑98 may directly target E2F transcription factor (E2F) 1, E2F2 and v‑myc avian myelocytomatosis viral oncogene homolog (MYC); miR‑10b may modulate peroxisome proliferator‑activated receptor α and Kruppel‑like factor 4; miR‑485‑5p and miR‑363 may regulate TNF receptor superfamily member 5 and cyclin‑dependent kinase inhibitor 1A. In addition, E2F1, E2F2 and MYC were associated with the cell cycle, which was the most significantly enriched function and pathway in EC. The results of the present study suggested that miR‑144, miR‑451, miR‑98, miR‑10b and miR‑363 may be involved in EC by regulating their target genes, and may be used as biomarkers for EC.
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Copy and paste a formatted citation
Spandidos Publications style
Du J and Zhang L: Analysis of salivary microRNA expression profiles and identification of novel biomarkers in esophageal cancer. Oncol Lett 14: 1387-1394, 2017.
APA
Du, J., & Zhang, L. (2017). Analysis of salivary microRNA expression profiles and identification of novel biomarkers in esophageal cancer. Oncology Letters, 14, 1387-1394. https://doi.org/10.3892/ol.2017.6328
MLA
Du, J., Zhang, L."Analysis of salivary microRNA expression profiles and identification of novel biomarkers in esophageal cancer". Oncology Letters 14.2 (2017): 1387-1394.
Chicago
Du, J., Zhang, L."Analysis of salivary microRNA expression profiles and identification of novel biomarkers in esophageal cancer". Oncology Letters 14, no. 2 (2017): 1387-1394. https://doi.org/10.3892/ol.2017.6328
Copy and paste a formatted citation
x
Spandidos Publications style
Du J and Zhang L: Analysis of salivary microRNA expression profiles and identification of novel biomarkers in esophageal cancer. Oncol Lett 14: 1387-1394, 2017.
APA
Du, J., & Zhang, L. (2017). Analysis of salivary microRNA expression profiles and identification of novel biomarkers in esophageal cancer. Oncology Letters, 14, 1387-1394. https://doi.org/10.3892/ol.2017.6328
MLA
Du, J., Zhang, L."Analysis of salivary microRNA expression profiles and identification of novel biomarkers in esophageal cancer". Oncology Letters 14.2 (2017): 1387-1394.
Chicago
Du, J., Zhang, L."Analysis of salivary microRNA expression profiles and identification of novel biomarkers in esophageal cancer". Oncology Letters 14, no. 2 (2017): 1387-1394. https://doi.org/10.3892/ol.2017.6328
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