Clinical significance of ZNF750 gene expression, a novel tumor suppressor gene, in esophageal squamous cell carcinoma

  • Authors:
    • Sho Nambara
    • Takaaki Masuda
    • Taro Tobo
    • Shinya Kidogami
    • Hisateru Komatsu
    • Keishi Sugimachi
    • Hiroshi Saeki
    • Eiji Oki
    • Yoshihiko Maehara
    • Koshi Mimori
  • View Affiliations

  • Published online on: June 8, 2017     https://doi.org/10.3892/ol.2017.6341
  • Pages: 1795-1801
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present authors previously identified a novel candidate tumor suppressor gene, zinc finger protein 750 (ZNF750), in esophageal squamous cell carcinoma (ESCC) (1). The present study aimed to clarify the clinical significance of ZNF750 expression in ESCC. The association between ZNF750 DNA mutation status and the mRNA expression was examined by whole exome sequence analysis and quantitative reverse transcription polymerase chain reaction (RT‑qPCR). The expression of ZNF750 in 76 patients with ESCC (Kyushu University Beppu Hospital) was measured using immunohistochemistry and RT‑qPCR. Using this dataset, the association between ZNF750 mRNA expression and clinicopathological factors was examined. Additionally, survival analysis was performed using datasets from the Kyushu University Beppu Hospital and The Cancer Genome Atlas (TCGA). The biological effects of ZNF750 expression were explored using gene set enrichment analysis (GSEA) and were validated using datasets from the Cancer Cell Line Encyclopedia (CCLE) and the Kyushu University Beppu Hospital. ZNF750 expression analyses demonstrated that ZNF750 mRNA expression was lower in patients with the DNA mutations compared with those without the mutations (P<0.05), and ZNF750 expression was downregulated in tumor tissues compared with normal tissues (P<0.00005). In the clinicopathological analysis, the low ZNF750 expression group exhibited a higher incidence of undifferentiated histology (P<0.05) compared with the high expression group. The low ZNF750 expression group exhibited a poorer prognosis in the Kyushu and TCGA datasets (P<0.0005 and P<0.05, respectively). GSEA indicated that ZNF750 expression was significantly correlated with epithelial differentiation in ESCC. This was confirmed using the datasets from CCLE and the Kyushu University Beppu Hospital by analyzing the levels of small proline rich protein 1A mRNA, an epithelial differentiation‑associated gene. In conclusion, the results of the present study suggested that ZNF750 serves a role as a tumor suppressor; potentially via regulating epithelial differentiation and that it may be a promising biomarker of poor outcomes in ESCC.
View Figures
View References

Related Articles

Journal Cover

August-2017
Volume 14 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Nambara S, Masuda T, Tobo T, Kidogami S, Komatsu H, Sugimachi K, Saeki H, Oki E, Maehara Y, Mimori K, Mimori K, et al: Clinical significance of ZNF750 gene expression, a novel tumor suppressor gene, in esophageal squamous cell carcinoma. Oncol Lett 14: 1795-1801, 2017
APA
Nambara, S., Masuda, T., Tobo, T., Kidogami, S., Komatsu, H., Sugimachi, K. ... Mimori, K. (2017). Clinical significance of ZNF750 gene expression, a novel tumor suppressor gene, in esophageal squamous cell carcinoma. Oncology Letters, 14, 1795-1801. https://doi.org/10.3892/ol.2017.6341
MLA
Nambara, S., Masuda, T., Tobo, T., Kidogami, S., Komatsu, H., Sugimachi, K., Saeki, H., Oki, E., Maehara, Y., Mimori, K."Clinical significance of ZNF750 gene expression, a novel tumor suppressor gene, in esophageal squamous cell carcinoma". Oncology Letters 14.2 (2017): 1795-1801.
Chicago
Nambara, S., Masuda, T., Tobo, T., Kidogami, S., Komatsu, H., Sugimachi, K., Saeki, H., Oki, E., Maehara, Y., Mimori, K."Clinical significance of ZNF750 gene expression, a novel tumor suppressor gene, in esophageal squamous cell carcinoma". Oncology Letters 14, no. 2 (2017): 1795-1801. https://doi.org/10.3892/ol.2017.6341