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Decrease in stathmin expression by arsenic trioxide inhibits the proliferation and invasion of osteosarcoma cells via the MAPK signal pathway

  • Authors:
    • Tao Feng
    • Jun Xu
    • Ping He
    • Yuanyuan Chen
    • Ruiying Fang
    • Xuejun Shao
  • View Affiliations / Copyright

    Affiliations: Clinical Laboratory, The Children's Hospital of Suzhou University, Suzhou, Jiangsu 215025, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1333-1340
    |
    Published online on: June 8, 2017
       https://doi.org/10.3892/ol.2017.6347
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Abstract

Osteosarcoma (OS) is the most common type of malignant bone tumor in children and adolescents. In total, 40‑50% of patients with OS experience metastasis, and thus have a poor prognosis. Our previous study demonstrated that arsenic trioxide (As2O3) combined with doxorubicin [also known as Adriamycin (ADM)] significantly inhibited OS cell proliferation by downregulating stathmin expression. The present study investigated the effect and mechanism of stathmin expression on OS cell invasion. It was identified that the expression of stathmin was increased in human ADM-resistant OS MG63 (MG63/dox) cells compared with the level in the normal osteoblast hFoB1.19cell line using western blot analysis. Lentiviral‑mediated small hairpin RNA (shRNA) was constructed to silence stathmin expression of MG63/dox cells. In transwell assay, stathmin‑knockdown significantly suppressed migration and invasion in MG63/dox cells. As2O3 combined with ADM inhibited the migration and invasion of MG63/dox cells, and was associated with the downregulation of phosphorylated-mitogen-activated protein kinase (MAPK) 1 and β‑catenin, and upregulation of phosphorylated‑MAPK8 and E‑cadherin. In addition, stathmin‑knockdown significantly suppressed tumor growth and increased E‑cadherin expression in a xenograft nude mouse model. Taken together, these data suggested that As2O3 combined with ADM inhibited stathmin-mediated invasion via the MAPK pathway. Elucidation of the mechanism for stathmin downregulation by As2O3 may provide novel insights into the mechanism of OS metastasis.
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View References

1 

Man TK, Chintagumpala M, Visvanathan J, Shen J, Perlaky L, Hicks J, Johnson M, Davino N, Murray J, Helman L, et al: Expression profiles of osteosarcoma that can predict response to chemotherapy. Cancer Res. 65:8142–8150. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Kim HJ, Chalmers PN and Morris CD: Pediatric osteogenic sarcoma. Curr Opin Pediatr. 22:61–66. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Hongtao L, Hui Z, Bingshun W, Xiaojin W, Zhiyu W, Shuier Z, Aina H, Yuanjue S, Daliu M, Zan S and Yang Y: 18F-FDG positron emission tomography for the assessment of histological response to neoadjuvant chemotherapy in osteosarcomas: A meta-analysis. Surg Oncol. 21:e165–e170. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Bachleitner-Hofmann T, Kees M and Gisslinger H: Arsenic trioxide: Acute promyelocytic leukemia and beyond. Leuk Lymphoma. 43:1535–1540. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Miller WH Jr, Schipper HM, Lee JS, Singer J and Waxman S: Mechanisms of action of arsenic trioxide. Cancer Res. 62:3893–3903. 2002.PubMed/NCBI

6 

Feng T, Qiao G, Feng L, Qi W, Huang Y, Yao Y and Shen Z: Stathmin is key in reversion of doxorubicin resistance by arsenic trioxide in osteosarcoma cells. Mol Med Rep. 10:2985–2992. 2014.PubMed/NCBI

7 

Niethammer P, Bastiaens P and Karsenti E: Stathmin-tubulin interaction gradients in motile and mitotic cells. Science. 303:1862–1866. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Hu JY, Chu ZG, Han J, Dang YM, Yan H, Zhang Q, Liang GP and Huang YS: The p38/MAPK pathway regulates microtubule polymerization through phosphorylation of MAP4 and Op18 in hypoxic cells. Cell Mol Life Sci. 67:321–333. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Schmalhofer O, Brabletz S and Brabletz T: E-cadherin, beta-catenin, and ZEB1 in malignant progression of cancer. Cancer Metastasis Rev. 28:151–166. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Chen HN, Yuan K, Xie N, Wang K, Huang Z, Chen Y, Dou Q, Wu M, Nice EC, Zhou ZG and Huang C: PDLIM1 stabilizes the E-cadherin/β-catenin complex to prevent epithelial-mesenchymal transition and metastatic potential of colorectal cancer cells. Cancer Res. 76:1122–1134. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Baldassarre G, Belletti B, Nicoloso MS, Schiappacassi M, Vecchione A, Spessotto P, Morrione A, Canzonieri V and Colombatti A: p27 (Kip1)-stathmin interaction influences sarcoma cell migration and invasion. Cancer Cell. 7:51–63. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Li N, Jiang P, Du W, Wu Z, Li C, Qiao M, Yang X and Wu M: Siva1 suppresses epithelial-mesenchymal transition and metastasis of tumor cells by inhibiting stathmin and stabilizing microtubules. Proc Natl Acad Sci USA. 108:pp. 12851–12856. 2011; View Article : Google Scholar : PubMed/NCBI

13 

Liu F, Sun YL, Xu Y, Liu F, Wang LS and Zhao XH: Expression and phosphorylation of stathmin correlate with cell migration in esophageal squamous cell carcinoma. Oncol Rep. 29:419–424. 2013.PubMed/NCBI

14 

Chandhanayingyong C, Kim Y, Staples JR, Hahn C and Lee FY: MAPK/ERK signaling in osteosarcomas, Ewing sarcomas and chondrosarcomas: Therapeutic implications and future directions. Sarcoma. 2012:4048102012. View Article : Google Scholar : PubMed/NCBI

15 

Miao JH, Wang SQ, Zhang MH, Yu FB, Zhang L, Yu ZX and Kuang Y: Knockdown of galectin-1 suppresses the growth and invasion of osteosarcoma cells through inhibition of the MAPK/ERK pathway. Oncol Rep. 32:1497–1504. 2014.PubMed/NCBI

16 

Howard S, Deroo T, Fujita Y and Itasaki N: A positive role of cadherin in Wnt/β-catenin signalling during epithelial-mesenchymal transition. PLoS One. 6:e238992011. View Article : Google Scholar : PubMed/NCBI

17 

Faux MC, Coates JL, Kershaw NJ, Layton MJ and Burgess AW: Independent interactions of phosphorylated β-catenin with E-cadherin at cell-cell contacts and APC at cell protrusions. PLoS One. 5:e141272010. View Article : Google Scholar : PubMed/NCBI

18 

Howard S, Deroo T, Fujita Y and Itasaki N: A positive role of cadherin in Wnt/β-catenin signalling during epithelial-mesenchymal transition. PLoS One. 6:e238992011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Feng T, Xu J, He P, Chen Y, Fang R and Shao X: Decrease in stathmin expression by arsenic trioxide inhibits the proliferation and invasion of osteosarcoma cells via the MAPK signal pathway. Oncol Lett 14: 1333-1340, 2017.
APA
Feng, T., Xu, J., He, P., Chen, Y., Fang, R., & Shao, X. (2017). Decrease in stathmin expression by arsenic trioxide inhibits the proliferation and invasion of osteosarcoma cells via the MAPK signal pathway. Oncology Letters, 14, 1333-1340. https://doi.org/10.3892/ol.2017.6347
MLA
Feng, T., Xu, J., He, P., Chen, Y., Fang, R., Shao, X."Decrease in stathmin expression by arsenic trioxide inhibits the proliferation and invasion of osteosarcoma cells via the MAPK signal pathway". Oncology Letters 14.2 (2017): 1333-1340.
Chicago
Feng, T., Xu, J., He, P., Chen, Y., Fang, R., Shao, X."Decrease in stathmin expression by arsenic trioxide inhibits the proliferation and invasion of osteosarcoma cells via the MAPK signal pathway". Oncology Letters 14, no. 2 (2017): 1333-1340. https://doi.org/10.3892/ol.2017.6347
Copy and paste a formatted citation
x
Spandidos Publications style
Feng T, Xu J, He P, Chen Y, Fang R and Shao X: Decrease in stathmin expression by arsenic trioxide inhibits the proliferation and invasion of osteosarcoma cells via the MAPK signal pathway. Oncol Lett 14: 1333-1340, 2017.
APA
Feng, T., Xu, J., He, P., Chen, Y., Fang, R., & Shao, X. (2017). Decrease in stathmin expression by arsenic trioxide inhibits the proliferation and invasion of osteosarcoma cells via the MAPK signal pathway. Oncology Letters, 14, 1333-1340. https://doi.org/10.3892/ol.2017.6347
MLA
Feng, T., Xu, J., He, P., Chen, Y., Fang, R., Shao, X."Decrease in stathmin expression by arsenic trioxide inhibits the proliferation and invasion of osteosarcoma cells via the MAPK signal pathway". Oncology Letters 14.2 (2017): 1333-1340.
Chicago
Feng, T., Xu, J., He, P., Chen, Y., Fang, R., Shao, X."Decrease in stathmin expression by arsenic trioxide inhibits the proliferation and invasion of osteosarcoma cells via the MAPK signal pathway". Oncology Letters 14, no. 2 (2017): 1333-1340. https://doi.org/10.3892/ol.2017.6347
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