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Article

MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line

  • Authors:
    • Yankun Liu
    • Sugui Han
    • Yuhui Li
    • Yan Liu
    • Di Zhang
    • Yufeng Li
    • Jinghua Zhang
  • View Affiliations / Copyright

    Affiliations: The Cancer Institute, Tangshan People's Hospital, Tangshan, Hebei 063001, P.R. China, Department of Nuclear Medicine Clinical Laboratory, Tangshan People's Hospital, Tangshan, Hebei 063001, P.R. China, Department of Neurosurgery, Tangshan People's Hospital, Tangshan, Hebei 063001, P.R. China, College of Life Sciences, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China
  • Pages: 1780-1786
    |
    Published online on: June 8, 2017
       https://doi.org/10.3892/ol.2017.6348
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Abstract

MicroRNAs (miRs) have been reported to be associated with the development of numerous types of cancer. However, the function of miRs in human ovarian carcinoma chemoresistance remains largely undefined. In the present study, cell chemotherapy combined with a Cell Counting Kit‑8 assay demonstrated that miR‑20a performed important roles in ovarian cancer cells chemoresistance. Flow cytometry, cellular proliferation assays and Transwell assays results revealed that the proliferation and migration rates of OVCAR3/DDP cells were increased in comparison with parental cells. Western blot analysis results suggested that epithelial‑mesenchymal transition (EMT) activated by miR‑20a contributed to OVCAR3/DDP cell migration. The present study highlighted the importance of miR‑20a in regulating the chemoresistant properties of OVCAR3 cells and promoting cisplatin‑resistant cell migration by activating EMT. The results of present study may therefore provide novel insights into reversing the chemoresistance of ovarian cancer and improving its treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Han S, Li Y, Liu Y, Zhang D, Li Y and Zhang J: MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line. Oncol Lett 14: 1780-1786, 2017.
APA
Liu, Y., Han, S., Li, Y., Liu, Y., Zhang, D., Li, Y., & Zhang, J. (2017). MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line. Oncology Letters, 14, 1780-1786. https://doi.org/10.3892/ol.2017.6348
MLA
Liu, Y., Han, S., Li, Y., Liu, Y., Zhang, D., Li, Y., Zhang, J."MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line". Oncology Letters 14.2 (2017): 1780-1786.
Chicago
Liu, Y., Han, S., Li, Y., Liu, Y., Zhang, D., Li, Y., Zhang, J."MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line". Oncology Letters 14, no. 2 (2017): 1780-1786. https://doi.org/10.3892/ol.2017.6348
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Han S, Li Y, Liu Y, Zhang D, Li Y and Zhang J: MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line. Oncol Lett 14: 1780-1786, 2017.
APA
Liu, Y., Han, S., Li, Y., Liu, Y., Zhang, D., Li, Y., & Zhang, J. (2017). MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line. Oncology Letters, 14, 1780-1786. https://doi.org/10.3892/ol.2017.6348
MLA
Liu, Y., Han, S., Li, Y., Liu, Y., Zhang, D., Li, Y., Zhang, J."MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line". Oncology Letters 14.2 (2017): 1780-1786.
Chicago
Liu, Y., Han, S., Li, Y., Liu, Y., Zhang, D., Li, Y., Zhang, J."MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line". Oncology Letters 14, no. 2 (2017): 1780-1786. https://doi.org/10.3892/ol.2017.6348
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