Suppression of the Wnt signaling pathway may contribute to the inhibition of proliferation of human hepatocellular carcinoma SMMC-7721 cells by esculetin

  • Authors:
    • Xinfeng Fan
    • Haiyuan Du
    • Yaogui Sun
    • Junbing Jiang
    • Zhirui Wang
    • Wei Yin
    • Kuohai Fan
    • Hongquan Li
  • View Affiliations

  • Published online on: June 9, 2017     https://doi.org/10.3892/ol.2017.6364
  • Pages: 1731-1736
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Abstract

The aims of the present study were to investigate the effects of esculetin on the proliferation of human hepatocellular carcinoma SMMC-7721 cells and to determine the underlying mechanism behind this activity. An MTT assay was used to assess cell proliferation, reverse transcription‑quantitative polymerase chain reaction was used to determine the relative mRNA expression levels of β‑catenin, c‑Myc and cyclin D1, and western blot analysis was utilized to determine the levels of the associated proteins. Compared with the dimethyl sulfoxide control, esculetin reduced the cell viability of SMMC‑7721 and HL‑7702 cells in a dose‑ and time‑dependent manner. Treatment of SMMC‑7721 cells with esculetin resulted in downregulation of the mRNA and protein levels of β‑catenin, c‑Myc and cyclin D1. Esculetin increased the phosphorylation of β‑catenin at Ser33/Ser37/Thr41 and inhibited the proliferation of human hepatoma SMMC‑7721 cells by suppressing the Wnt signaling pathway. The results of the present study suggest that esculetin inhibited the Wnt/β‑catenin signaling pathway in SMMC-7721 cells and may have potential as an effective anti‑cancer drug, acting to inhibit the Wnt/β‑catenin signaling pathway.
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August-2017
Volume 14 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Fan X, Du H, Sun Y, Jiang J, Wang Z, Yin W, Fan K and Li H: Suppression of the Wnt signaling pathway may contribute to the inhibition of proliferation of human hepatocellular carcinoma SMMC-7721 cells by esculetin. Oncol Lett 14: 1731-1736, 2017
APA
Fan, X., Du, H., Sun, Y., Jiang, J., Wang, Z., Yin, W. ... Li, H. (2017). Suppression of the Wnt signaling pathway may contribute to the inhibition of proliferation of human hepatocellular carcinoma SMMC-7721 cells by esculetin. Oncology Letters, 14, 1731-1736. https://doi.org/10.3892/ol.2017.6364
MLA
Fan, X., Du, H., Sun, Y., Jiang, J., Wang, Z., Yin, W., Fan, K., Li, H."Suppression of the Wnt signaling pathway may contribute to the inhibition of proliferation of human hepatocellular carcinoma SMMC-7721 cells by esculetin". Oncology Letters 14.2 (2017): 1731-1736.
Chicago
Fan, X., Du, H., Sun, Y., Jiang, J., Wang, Z., Yin, W., Fan, K., Li, H."Suppression of the Wnt signaling pathway may contribute to the inhibition of proliferation of human hepatocellular carcinoma SMMC-7721 cells by esculetin". Oncology Letters 14, no. 2 (2017): 1731-1736. https://doi.org/10.3892/ol.2017.6364