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Article Open Access

Protoporphyrin IX-mediated sonodynamic therapy promotes autophagy in vascular smooth muscle cells

  • Authors:
    • Qingsong Li
    • Jian Kang
    • Xiaojing Xiong
    • Yan Liu
    • Wenwu Cao
    • Ying Liu
    • Yongning Li
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 160011, P.R. China, Department of Emergency, The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 160011, P.R. China, Materials Research Institute, Pennsylvania State University, University Park, PA 16802, USA
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2097-2102
    |
    Published online on: June 16, 2017
       https://doi.org/10.3892/ol.2017.6394
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Abstract

Sonodynamic therapy (SDT) is effective in treating intimal hyperplasia and promoting plaque stability in animal models. The present study aimed to evaluate the effects of SDT with the sonosensitizer protoporphyrin IX (PpIX) on vascular smooth muscle cell (VSMC) viability and autophagy. Cultured VSMCs cells were divided into the following groups: i) Control, ii) ultrasound, iii) PpIX and iv) SDT. Flow cytometry and laser confocal detection were used to measure Annexin V stained VSMCs following different treatments. Alterations in mitochondrial membrane potential (MMP) were evaluated via JC‑1 staining. Autophagosome formation was observed using electron and fluorescence microscopy. Western blotting was used to analyze the expression levels of the autophagy markers light chain 3 (LC3‑I) and LC3‑II. The results demonstrated that SDT did not trigger apoptosis nor induce a significant decline in MMP of VSMCs. However, SDT significantly increased autophagasome formation and increased the LC3‑II/LC3‑I ratio. The findings demonstrated that PpIX‑SDT increased autophagy without inducing mitochondrial‑dependent apoptosis in VSMCs.
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View References

1 

Perelman A, Wachtel C, Cohen M, Haupt S, Shapiro H and Tzur A: JC-1: Alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry. Cell Death Dis. 3:e4302012. View Article : Google Scholar : PubMed/NCBI

2 

Chen H, Gao W, Yang Y, Guo S, Wang H, Wang W, Zhang S, Zhou Q, Xu H, Yao J, et al: Inhibition of VDAC1 prevents Ca2+-mediated oxidative stress and apoptosis induced by 5-aminolevulinic acid mediated sonodynamic therapy in THP-1 macrophages. Apoptosis. 19:1712–1726. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Guo S, Sun X, Cheng J, Xu H, Dan J, Shen J, Zhou Q, Zhang Y, Meng L, Cao W and Tian Y: Apoptosis of THP-1 macrophages induced by protoporphyrin IX-mediated sonodynamic therapy. Int J Nanomedicine. 8:2239–2246. 2013.PubMed/NCBI

4 

Li X, Gao L, Zheng L, Kou J, Zhu X, Jiang Y, Zhong Z, Dan J, Xu H, Yang Y, et al: The efficacy and mechanism of apoptosis induction by hypericin-mediated sonodynamic therapy in THP-1 macrophages. Int J nanomedicine. 10:821–838. 2015.PubMed/NCBI

5 

Li Z, Sun X, Guo S, Wang L, Wang T, Peng C, Wang W, Tian Z, Zhao R, Cao W and Tian Y: Rapid stabilisation of atherosclerotic plaque with 5-aminolevulinic acid-mediated sonodynamic therapy. Thromb Haemost. 4:793–803. 2015. View Article : Google Scholar

6 

Sun X, Xu H, Shen J, Guo S, Shi S, Dan J, Tian F and Tian Y and Tian Y: Real-time detection of intracellular reactive oxygen species and mitochondrial membrane potential in THP-1 macrophages during ultrasonic irradiation for optimal sonodynamic therapy. Ultrason Sonochem. 22:7–14. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Krammer B and Plaetzer K: ALA and its clinical impact, from bench to beside. Photochem Photobiol Sci. 7:283–289. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Peng C, Li Y, Liang H, Cheng J, Li Q, Sun X, Li Z, Wang F, Guo Y, Tian Z, et al: Detection and photodynamic therapy of inflamed atherosclerotic plaques in the carotid artery of rabbits. J Photochem Photobiol B. 102:26–31. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Mizushima N, Yoshimori T and Levine B: Methods in mammalian autophagy research. Cell. 140:313–326. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Martinet W and De Meyer GR: Autophagy in atherosclerosis. Curr Atheroscler Rep. 10:216–223. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Lavandero S, Chiong M, Rothermel BA and Hill JA: Autophagy in cardiovascular biology. J Clin Invest. 125:55–64. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Ouimet M, Franklin V, Mak E, Liao X, Tabas I and Marcel YL: Autophagy regulates cholesterol efflux from macrophage foam cells via lysosomal acid lipase. Cell Metab. 13:655–667. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Li BH, Yin YW, Liu Y, Pi Y, Guo L, Cao XJ, Gao CY, Zhang LL and Li JC: TRPV1 activation impedes foam cell formation by inducing autophagy in oxLDL-treated vascular smooth muscle cells. Cell Death Dis. 5:e11822014. View Article : Google Scholar : PubMed/NCBI

14 

Michiels CF, Fransen P, De Munck DG, De Meyer GR and Martinet W: Defective autophagy in vascular smooth muscle cells alters contractility and Ca2+ homeostasis in mice. Am J Physiol Heart Circ Physiol. 308:H557–H567. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Dan J, Sun X, Li W, Zhang Y, Li X, Xu H, Li Z, Tian Z, Guo S, Yao J, et al: 5-Aminolevulinic acid-mediated sonodynamic therapy promotes phenotypic switching from dedifferentiated to differentiated phenotype via reactive oxygen species and p38 mitogen-activated protein kinase in vascular smooth muscle cells. Ultrasound Med Biol. 41:1681–1689. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Hagiya Y, Fukuhara H, Matsumoto K, Endo Y, Nakajima M, Tanaka T, Okura I, Kurabayashi A, Furihata M, Inoue K, et al: Expression levels of PEPT1 and ABCG2 play key roles in 5-aminolevulinic acid (ALA)-induced tumor-specific protoporphyrin IX (PpIX) accumulation in bladder cancer. Photodiagnosis Photodyn Ther. 10:288–295. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Uto Y, Tamatani D, Mizuki Y, Endo Y, Nakanishi I, Ohkubo K, Fukuzumi S, Ishizuka M, Tanaka T, Kuchiike D, et al: Evaluation of the sonosensitizing activities of 5-aminolevulinic acid and Sn(IV) chlorin e6 in tumor-bearing chick embryos. Anticancer Res. 34:4583–4587. 2014.PubMed/NCBI

18 

Cheng J, Sun X, Guo S, Cao W, Chen H, Jin Y, Li B, Li Q, Wang H, Wang Z, et al: Effects of 5-aminolevulinic acid-mediated sonodynamic therapy on macrophages. Int J Nanomedicine. 8:669–676. 2013.PubMed/NCBI

19 

Li Y, Zhou Q, Hu Z, Yang B, Li Q, Wang J, Zheng J and Cao W: 5-Aminolevulinic acid-based sonodynamic therapy induces the apoptosis of osteosarcoma in mice. PLoS One. 10:e01320742015. View Article : Google Scholar : PubMed/NCBI

20 

Kundu M and Thompson CB: Autophagy: Basic principles and relevance to disease. Annu Rev Pathol. 3:427–455. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Luo Y, Lu S, Zhou P, Ai QD, Sun GB and Sun XB: Autophagy: An exposing therapeutic target in atherosclerosis. J Cardiovasc Pharmacol. 67:266–274. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Perrotta I and Aquila S: The role of oxidative stress and autophagy in atherosclerosis. Oxid Med Cell Longev. 2015:1303152015. View Article : Google Scholar : PubMed/NCBI

23 

Liao X, Sluimer JC, Wang Y, Subramanian M, Brown K, Pattison JS, Robbins J, Martinez J and Tabas I: Macrophage autophagy plays a protective role in advanced atherosclerosis. Cell Metab. 15:545–553. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Mehta JL, Chen J, Hermonat PL, Romeo F and Novelli G: Lectin-like, oxidized low-density lipoprotein receptor-1 (Lox-1): A critical player in the development of atherosclerosis and related disorders. Cardiovasc Res. 69:36–45. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Xu K, Yang Y, Yan M, Zhan J, Fu X and Zheng X: Autophagy plays a protective role in free cholesterol overload-induced death of smooth muscle cells. J Lipid Res. 51:2581–2590. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Salabei JK, Cummins TD, Singh M, Jones SP, Bhatnagar A and Hill BG: PDGF-mediated autophagy regulates vascular smooth muscle cell phenotype and resistance to oxidative stress. Biochem J. 451:375–388. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Mahmoudi MJ, Mahmoudi M, Siassi F, Shokri F, Eshraghian MR, Zarnani AH, Chahardoli R, Hedayat M, Khoshnoodi J, Nayeri H, et al: Lymphocyte cytotoxicity of oxLDL in patients with atherosclerosis. Iran J Immunol. 8:27–33. 2011.PubMed/NCBI

28 

Mehta JL, Chen J, Hermonat PL, Romeo F and Novelli G: Lectin-like, oxidized low-density lipoprotein receptor-1 (LOX-1): A critical player in the development of atherosclerosis and related disorders. Cardiovasc Res. 69:36–45. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Martinet W and De Meyer GR: Autophagy in atherosclerosis: A cell survival and death phenomenon with therapeutic potential. Circ Res. 104:304–317. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, Kominami E, Ohsumi Y and Yoshimori T: LC3, a mammalian homogue of yeast Apg8p, is localizing in autophagosome membranes after processing. EMBO J. 19:5720–5728. 2000. View Article : Google Scholar : PubMed/NCBI

31 

Grootaert MO, da Costa Martins PA, Bitsch N, Pintelon I, De Meyer GR, Martinet W and Schrijvers DM: Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis. Autophagy. 11:2014–2032. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Klionsky DJ, Abeliovich H, Agostinis P, Agrawal DK, Aliev G, Askew DS, Baba M, Baehrecke EH, Bahr BA, Ballabio A, et al: Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy. 4:151–175. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Salabei JK, Cummins TD, Singh M, Jones SP, Bhatnagar A and Hill BG: PDGF-mediated autophagy regulates vascular smooth muscle cell phenotype and resistance to oxidative stress. Biochem J. 451:375–388. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li Q, Kang J, Xiong X, Liu Y, Cao W, Liu Y and Li Y: Protoporphyrin IX-mediated sonodynamic therapy promotes autophagy in vascular smooth muscle cells. Oncol Lett 14: 2097-2102, 2017.
APA
Li, Q., Kang, J., Xiong, X., Liu, Y., Cao, W., Liu, Y., & Li, Y. (2017). Protoporphyrin IX-mediated sonodynamic therapy promotes autophagy in vascular smooth muscle cells. Oncology Letters, 14, 2097-2102. https://doi.org/10.3892/ol.2017.6394
MLA
Li, Q., Kang, J., Xiong, X., Liu, Y., Cao, W., Liu, Y., Li, Y."Protoporphyrin IX-mediated sonodynamic therapy promotes autophagy in vascular smooth muscle cells". Oncology Letters 14.2 (2017): 2097-2102.
Chicago
Li, Q., Kang, J., Xiong, X., Liu, Y., Cao, W., Liu, Y., Li, Y."Protoporphyrin IX-mediated sonodynamic therapy promotes autophagy in vascular smooth muscle cells". Oncology Letters 14, no. 2 (2017): 2097-2102. https://doi.org/10.3892/ol.2017.6394
Copy and paste a formatted citation
x
Spandidos Publications style
Li Q, Kang J, Xiong X, Liu Y, Cao W, Liu Y and Li Y: Protoporphyrin IX-mediated sonodynamic therapy promotes autophagy in vascular smooth muscle cells. Oncol Lett 14: 2097-2102, 2017.
APA
Li, Q., Kang, J., Xiong, X., Liu, Y., Cao, W., Liu, Y., & Li, Y. (2017). Protoporphyrin IX-mediated sonodynamic therapy promotes autophagy in vascular smooth muscle cells. Oncology Letters, 14, 2097-2102. https://doi.org/10.3892/ol.2017.6394
MLA
Li, Q., Kang, J., Xiong, X., Liu, Y., Cao, W., Liu, Y., Li, Y."Protoporphyrin IX-mediated sonodynamic therapy promotes autophagy in vascular smooth muscle cells". Oncology Letters 14.2 (2017): 2097-2102.
Chicago
Li, Q., Kang, J., Xiong, X., Liu, Y., Cao, W., Liu, Y., Li, Y."Protoporphyrin IX-mediated sonodynamic therapy promotes autophagy in vascular smooth muscle cells". Oncology Letters 14, no. 2 (2017): 2097-2102. https://doi.org/10.3892/ol.2017.6394
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